Rapid Fabrication of Bio‐inspired 3D Microfluidic Vascular Networks

Kim, Jeongyun; Agrawal, Nitin; Sudarsan, Arjun P.; Maxim, Joseph E.; Jayaraman, Arul; Ugaz, Victor M.

doi:10.1002/adma.200900584

Cited by 103 publications

(60 citation statements)

References 37 publications

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“…Inspired by the natural fractal tree-like electrostatic discharge path, as seen in the lightening from the sky, Huang and colleagues exploited the hierarchy of electric discharge phenomenon to rapidly create 3D vascular network within plastic-like materials such as PMMA and PLA (poly(lactic acid)) (Figure 14e&f). [298] Upon electron beam irradiation, a large amount of charge was accumulated within the material such that the energy released during the discharge was high enough to vaporize and fracture the surrounding materials along the discharge path. The subsequent electrostatic discharge was initiated either exogenously using a sharp tip of a grounded electrode or spontaneously by a nucleation defect introduced into the material before the irradiation.…”

Section: Toolbox Of Micro/nanoengineered Functional Biomaterialsmentioning

confidence: 99%

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Integrated Micro/Nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective

2013

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The rapid development of micro/nanoengineered functional biomaterials in the last two decades has empowered materials scientists and bioengineers to precisely control different aspects of the in vitro cell microenvironment. Following a philosophy of reductionism, many studies using synthetic functional biomaterials have revealed instructive roles of individual extracellular biophysical and biochemical cues in regulating cellular behaviors. Development of integrated micro/nanoengineered functional biomaterials to study complex and emergent biological phenomena has also thrived rapidly in recent years, revealing adaptive and integrated cellular behaviors closely relevant to human physiological and pathological conditions. Working at the interface between materials science and engineering, biology, and medicine, we are now at the beginning of a great exploration using micro/nanoengineered functional biomaterials for both fundamental biology study and clinical and biomedical applications such as regenerative medicine and drug screening. In this review, we present an overview of state of the art micro/nanoengineered functional biomaterials that can control precisely individual aspects of cell-microenvironment interactions and highlight them as well-controlled platforms for mechanistic studies of mechano-sensitive and -responsive cellular behaviors and integrative biology research. We also discuss the recent exciting trend where micro/nanoengineered biomaterials are integrated into miniaturized biological and biomimetic systems for dynamic multiparametric microenvironmental control of emergent and integrated cellular behaviors. The impact of integrated micro/nanoengineered functional biomaterials for future in vitro studies of regenerative medicine, cell biology, as well as human development and disease models are discussed.

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Section: Toolbox Of Micro/nanoengineered Functional Biomaterialsmentioning

confidence: 99%

“…(e) Schematic of a rapid fabrication process of 3D hierarchical vascular network via electrostatic discharge in plastic-like materials (e.g., PMMA and acrylic). [298] (f) Photograph of a hierarchical vascular network fabricated within an acrylic block. [298] Reproduced with permission from [298].…”

Section: Figurementioning

confidence: 99%

Integrated Micro/Nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective

2013

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“…The etching rate is influenced by parameters including temperature, local velocity, and enzyme concentration, suggesting a high degree of flexibility to produce locally tailored cross-sectional topologies via imposition of gradients and selection of the initial pre-etching geometry. These capabilities may be particularly useful in construction of bio-inspired 3D branched networks, 17,18 enabling a variety of cell-favored interior environments to be produced using only a single template.…”

Section: Discussionmentioning

confidence: 99%

Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies

et al. 2016

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The ability to harness enzymatic activity as an etchant to precisely machine biodegradable substrates introduces new possibilities for microfabrication. This flow-based etching is straightforward to implement, enabling patterning of microchannels with topologies that incorporate variable depth along the cross-sectional dimension. Additionally, unlike conventional small-molecule formulations, the macromolecular nature of enzymatic etchants enables features to be precisely positioned. Here, we introduce a kinetic model to characterize the enzymatic machining process and its localization by co-injection of a macromolecular inhibitor species. Our model captures the interaction between enzyme, inhibitor, and substrate under laminar flow, enabling rational prediction of etched microchannel profiles so that cross-sectional topologies incorporating complex lateral variations in depth can be constructed. We also apply this approach to achieve simultaneous widening of an entire network of microchannels produced in the biodegradable polymeric substrate poly(lactic acid), laying a foundation to construct systems incorporating a broad range of internal cross-sectional dimensions by manipulating the process conditions. Published by AIP Publishing. [http://dx

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“…Murray's law). Huang et al (2009) irradiated poly (methyl methacrylate) (PMMA) with an electron beam, causing electrical charges to accumulate inside the material. The specimen was then connected to the ground and a process of rapid discharge similar to lightning occurred, creating a tree-shape branched microvascular network inside the specimen block, as shown in Figure 8.…”

Section: Electrostatic Dischargementioning

confidence: 99%

Self-healing composites: A review

2015

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Self-healing composites are composite materials capable of automatic recovery when damaged. They are inspired by biological systems such as the human skin which are naturally able to heal themselves. This paper reviews work on selfhealing composites with a focus on capsule-based and vascular healing systems. Complementing previous survey articles, the paper provides an updated overview of the various self-healing concepts proposed over the past 15 years, and a comparative analysis of healing mechanisms and fabrication techniques for building capsules and vascular networks. Based on the analysis, factors that influence healing performance are presented to reveal key barriers and potential research directions.

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Rapid Fabrication of Bio‐inspired 3D Microfluidic Vascular Networks

Cited by 103 publications

References 37 publications

Integrated Micro/Nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective

Integrated Micro/Nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective

Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies

Self-healing composites: A review

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