Rapid fabrication of poly(DL-lactide) nanofiber scaffolds with tunable degradation for tissue engineering applications by air-brushing

Solution blow spinning (SBS) is a technique that can be used to deposit fibers in situ at low cost for a variety of applications, which include biomedical materials and flexible electronics. This review is intended to provide an overview of the basic principles and applications of SBS. We first describe a method for creating a spinnable polymer solution and stable polymer solution jet by manipulating parameters such as polymer concentration and gas pressure. This method is based on fundamental insights, theoretical models, and empirical studies. We then discuss the unique bundled morphology and mechanical properties of fiber mats produced by SBS, and how they compare with electrospun fiber mats. Applications of SBS in biomedical engineering are highlighted, showing enhanced cell infiltration and proliferation versus electrospun fiber scaffolds and in situ deposition of biodegradable polymers. We also discuss the impact of SBS in applications involving textiles and electronics, including ceramic fibers and conductive composite materials. Strategies for future research are presented that take advantage of direct and rapid polymer deposition via cost-effective methods.

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“…19 Porosity and fiber branching can be controlled using process variables such as polymer concentration and polymer blending. 26,37 …”

Section: Structure and Properties Of Fibersmentioning

confidence: 99%

A Review of the Fundamental Principles and Applications of Solution Blow Spinning

Daristotle

Behrens

Sandler

et al. 2016

ACS Appl. Mater. Interfaces

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323

242

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“…Since the 1990s there has been an increasing interest in methods for polymeric fibres production, such as electrospinning [3]. Recently, nanometric fibres from a variety of polymers, including polyvinyl chloride (PVC), nylon-6, poly(lactic acid) (PLA) and poly(D, L-lactic acid) (PDLLA) have been produced by another method known as solution blow spinning (SBS) [4][5][6][7][8][9][10][11]. This innovative technique is conceptually similar to electrospinning but does not require a high voltage and, therefore, it is much safer and simpler.…”

Section: Introductionmentioning

confidence: 99%

Solution blow spinning fibres: New immunologically inert substrates for the analysis of cell adhesion and motility

et al. 2017

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The control of cell behaviour through material geometry is appealing as it avoids the requirement for complex chemical surface modifications. Significant advances in new technologies have been made to the development of polymeric biomaterials with controlled geometry and physico-chemical properties. Solution blow spinning technique has the advantage of ease of use allowing the production of nano or microfibres and the direct fibre deposition on any surface in situ. Yet, in spite of these advantages, very little is known about the influence of such fibres on biological functions such as immune response and cell migration. In this work, we engineered polymeric fibres composed of either pure poly(lactic acid) (PLA) or blends of PLA and polyethylene glycol (PEG) by solution blow spinning and determined their impact on dendritic cells, highly specialised cells essential for immunity and tolerance. We also determined the influence of fibres on cell adhesion and motility. Cells readily interacted with fibres resulting in an intimate contact characterised by accumulation of actin filaments and focal adhesion components at sites of cell-fibre interactions. Moreover, cells were guided along the fibres and actin and focal adhesion components showed a highly dynamic behaviour at cell-fibre interface. Remarkably, fibres did not elicit any substantial increase of activation markers and inflammatory cytokines in dendritic cells, which remained in their immature (inactive) state. Taken together, these findings will be useful for developing new biomaterials for applications in tissue engineering and regenerative medicine.

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“…Decellularised tissue brings favourable physical and chemical characteristics to help support cells and control physiology [47], but as cell produce their own ECM a synthetic scaffold can provide an excellent foundation to build upon [48,49]. Polymer scaffolds offer many benefits over decellularised tissue as their manufacture can be highly controlled, as well as providing superior mechanical properties with degradation over a tuneable time frame [31,33,50].…”

Section: Discussionmentioning

confidence: 99%

A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

Burton

Callanan

2018

Tissue Eng Regen Med

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Chronic kidney disease is a major global health problem affecting millions of people; kidney tissue engineering provides an opportunity to better understand this disease, and has the capacity to provide a cure. Two-dimensional cell culture and decellularised tissue have been the main focus of this research thus far, but despite promising results these methods are not without their shortcomings. Polymer fabrication techniques such as electrospinning have the potential to provide a non-woven path for kidney tissue engineering. In this experiment we isolated rat primary kidney cells which were seeded on electrospun poly(lactic acid) scaffolds. Our results showed that the scaffolds were capable of sustaining a multipopulation of kidney cells, determined by the presence of: aquaporin-1 (proximal tubules), aquaporin-2 (collecting ducts), synaptopodin (glomerular epithelia) and von Willebrand factor (glomerular endothelia cells), viability of cells appeared to be unaffected by fibre diameter. The ability of electrospun polymer scaffold to act as a conveyor for kidney cells makes them an ideal candidate within kidney tissue engineering; the non-woven path provides benefits over decellularised tissue by offering a high morphological control as well as providing superior mechanical properties with degradation over a tuneable time frame.

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Rapid fabrication of poly(DL-lactide) nanofiber scaffolds with tunable degradation for tissue engineering applications by air-brushing

Cited by 24 publications

References 35 publications

A Review of the Fundamental Principles and Applications of Solution Blow Spinning

A Review of the Fundamental Principles and Applications of Solution Blow Spinning

Solution blow spinning fibres: New immunologically inert substrates for the analysis of cell adhesion and motility

A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

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