Rapid Fermentation Process Development and Optimization

Sun, Jun; Chew, Lawrence

doi:10.1002/9781118433034.ch5

Cited by 1 publication

(1 citation statement)

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“…The issue of controlled cell physiology and process robustness, i.e. limitation of batch-to-batch variation, is well considered in industrial bioprocesses where glucose based mineral salt media are normally used and complex additives are avoided [ 13 , 14 ]. Control of the cell metabolism is obtained by continuous feed of the only carbon source in a way that the feeding rate limits the uptake rate.…”

Section: Introductionmentioning

confidence: 99%

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

Krause

Neubauer²,

Neubauer

2016

Microb Cell Fact

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While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to the difficulty and expenses to apply a controlled continuous glucose feed to shaken cultures. New ready-made growth media, e.g. by biocatalytic release of glucose from a polymer, offer a simple solution for the application of the fed-batch principle in shaken plate and flask cultures. Their wider use has shown that the controlled diet not only provides a solution to obtain significantly higher cell yields, but also in many cases folding of the target protein is improved by the applied lower growth rates; i.e. final volumetric yields for the active protein can be a multiple of what is obtained in complex medium cultures. The combination of the conventional optimization approaches with new and easy applicable growth systems has revolutionized recombinant protein production in Escherichia coli in view of product yield, culture robustness as well as significantly increased cell densities. This technical development establishes the basis for successful miniaturization and parallelization which is now an important tool for synthetic biology and protein engineering approaches. This review provides an overview of the recent developments, results and applications of advanced growth systems which use a controlled glucose release as substrate supply.

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Section: Introductionmentioning

confidence: 99%