Rapid fingerprinting of sterols and related compounds in vegetable and animal oils and phytosterol enriched- margarines by transmission mode direct analysis in real time mass spectrometry

Alberici, Rosana M.; Fernandes, Gabriel D.; Porcari, Andréia M.; Eberlin, Marcos N.; Barrera‐Arellano, Daniel; Fernández, Facundo M.

doi:10.1016/j.foodchem.2016.05.057

Cited by 52 publications

(27 citation statements)

References 27 publications

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“…Phytosterols have anti-inflammatory, antibacterial, antifungal, antiulcerogenic and anti-tumoral activity [18]. Mammals do not synthesize phytosterols.…”

Section: Discussionmentioning

confidence: 99%

Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke

Sivrikaya

Öztürk

Ekmekçi

et al. 2018

Revista Romana De Medicina De Laborator

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“…Phytosterols have anti-inflammatory, antibacterial, antifungal, antiulcerogenic and anti-tumoral activity [18]. Mammals do not synthesize phytosterols.…”

Section: Discussionmentioning

confidence: 99%

Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke

Sivrikaya

Öztürk

Ekmekçi

et al. 2018

Revista Romana De Medicina De Laborator

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“…166 By means of TM-DART-MS, vegetable oils, phytosterols enriched margarines, butters and animal oil were satisfactorily differentiated with characteristic ions for each sample type, allowing sample classification based on phytosterol profiles. 167 …”

Section: Foodomicsmentioning

confidence: 99%

Ambient mass spectrometry in metabolomics

Clendinen

Monge

Fernández

2017

Analyst

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Since the introduction of desorption electrospray ionization (DESI) mass spectrometry (MS), ambient MS methods have seen increased use in a variety of fields from health to food science. Increasing its popularity in metabolomics, ambient MS offers limited sample preparation, rapid and direct analysis of liquids, solids, and gases, in situ and in vivo analysis, and imaging. The metabolome consists of a constantly changing collection of small (<1.5KDa) molecules. These include endogenous molecules that are part of primary metabolism pathways, secondary metabolites with specific functions such as signaling, chemicals incorporated in the diet or resulting from environmental exposures, and metabolites associated with the microbiome. Characterization of the responsive changes of this molecule cohort is the principle goal in any metabolomics study. With adjustments to experimental parameters, metabolites across a range of chemical and physical properties can be selectively desorbed and ionized and subsequently analyzed with increased speed and sensitivity. This review covers the broad applications of a variety of ambient MS techniques in four primary fields in which metabolomics is commonly employed.

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“…reproducible nets and/or meshes) to normalize the introduction of samples into the mass spectrometer, which allows for a more repeatable response from the DART‐MS system and facilitates its implementation as an HT technology. Nonetheless, the potential of TM has yet to be fully explored, with only a limited number of research papers having been published to date . In contrast, a number of publications have documented the implementation of DART for HT applications via the use of other sample‐introduction methods.…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, the potential of TM has yet to be fully explored, with only a limited number of research papers having been published to date. [15][16][17] In contrast, a number of publications have documented the implementation of DART for HT applications via the use of other sample-introduction methods. For example, Danhelova et al analyzed caffeine using a simple exposure method wherein dip-it tips® were inserted into a hot water/coffee mixture and then processed using an AUTO/DART-96, which is a robotic system from CTC Analytics.…”

Section: Introductionmentioning

confidence: 99%

High‐throughput quantification of drugs of abuse in biofluids via 96‐solid‐phase microextraction–transmission mode and direct analysis in real time mass spectrometry

Vasiljević

Gómez‐Ríos

Li³

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale The workload of clinical laboratories has been steadily increasing over the last few years. High‐throughput (HT) sample processing allows scientists to spend more time undertaking matters of critical thinking rather than laborious sample processing. Herein we introduce a HT 96‐solid‐phase microextraction (SPME) transmission mode (TM) system coupled to direct analysis in real time (DART) mass spectrometry (MS). Methods Model compounds (opioids) were extracted from urine and plasma samples using a 96‐SPME‐TM device. A standard voltage and pressure (SVP) DART source was used for all experiments. Examination of SPME‐TM performance was done using high‐resolution mass spectrometry (HRMS) in full scan mode (100–500 m/z), whereas quantitation of opioids was performed using triple quadrupole MS in multiple reaction monitoring mode and by using a matrix‐matched internal standard correction method. Results Thirteen points (0.5 to 200 ng mL−1) were used to establish a calibration curve. Low limits of quantitation (LOQ) were obtained (0.5 to 25 ng mL−1) for matrices used. Acceptable accuracy (71.4–129.4%) and repeatability (1.1–24%) were obtained for validation levels tested (0.5, 30 and 90 ng mL−1). In less than 1.5 hours, 96 samples were extracted, desorbed and processed using the 96‐SPME‐TM system coupled to DART‐MS. Conclusions A rapid HT method for detection of opioids in urine and plasma samples was developed. This study demonstrated that ambient ionization mass spectrometry coupled to robust sample preparation methods such as SPME‐TM can rapidly and efficiently screen/quantify target analytes in a HT context.

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Rapid fingerprinting of sterols and related compounds in vegetable and animal oils and phytosterol enriched- margarines by transmission mode direct analysis in real time mass spectrometry

Cited by 52 publications

References 27 publications

Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke

Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke

Ambient mass spectrometry in metabolomics

High‐throughput quantification of drugs of abuse in biofluids via 96‐solid‐phase microextraction–transmission mode and direct analysis in real time mass spectrometry

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