Rapid functionalization of cantilever array sensors by inkjet printing

Bietsch, Alexander; Zhang, Jiayun; Hegner, Martin; Lang, Hans Peter; Gerber, Christoph

doi:10.1088/0957-4484/15/8/002

Cited by 222 publications

(152 citation statements)

References 27 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…The features of the system match major requirements of protein array techniques, such as a miniaturized format, low consumption of analyte, real-time measurements, and the parallelization of the procedure. Implementation of new cantilever-coating technologies, such as inkjet printing (40), and new array formats comprising thousands of cantilevers, like the high-density 2D array reported recently (41), opens up opportunities for scaling up the procedure.…”

Section: Discussionmentioning

confidence: 99%

A label-free immunosensor array using single-chain antibody fragments

Backmann

Zahnd

Huber

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

266

183

View full text Add to dashboard Cite

We report a microcantilever-based immunosensor operated in static deflection mode with a performance comparable with surface plasmon resonance, using single-chain Fv (scFv) antibody fragments as receptor molecules. As a model system scFv fragments with specificity to two different antigens were applied. We introduced a cysteine residue at the C terminus of each scFv construct to allow covalent attachment to gold-coated sensor interfaces in directed orientation. Application of an array enabled simultaneous deflection measurements of sensing and reference cantilevers. The differential deflection signal revealed specific antigen binding and was proportional to the antigen concentration in solution. Using small, oriented scFv fragments as receptor molecules we increased the sensitivity of microcantilevers to Ϸ1 nM.cantilever arrays ͉ nanomechanics ͉ proteomics M icrocantilever-based sensors have attracted much interest as devices for fast and reliable detection of small amounts of molecules in air and solution. Over the last few years the application of the cantilever sensor concept was extended to the measurements of biocompounds in solution, resulting in a versatile biosensor (1, 2). Because of its label-free detection principle and small size, this kind of biosensor is advantageous for diagnostic applications, disease monitoring, and research in genomics or proteomics (3, 4). Multicantilever arrays would enable the detection of several analytes simultaneously.The main principle of the cantilever static mode is the transduction of the molecular interaction between analyte and receptors, immobilized as a layer on one surface of a cantilever, into a nanomechanical motion of the cantilever. Biomolecular interactions taking place on a solid-state interface produce a change in surface stress, because of changes in molecular configuration and intermolecular crowding (5). This process results in bending of the cantilever. Microcantilever-based biosensors operated in static mode have been successfully applied for the detection of various molecular interactions such as ssDNA-ssDNA (5-7) or protein-DNA (8, 9). Interactions between proteins were detected with cantilever-based immunosensors, where an antigen was recognized by its cognate antibody randomly immobilized on the sensor surface (10-12).The most critical step in preparation of any immunosensor is the immobilization of capture molecules on the support, a process where the orientation of the antigen-binding sites toward the analyte in solution plays a key role. Immunoglobulins can be either adsorbed on gold directly (10, 12) or attached covalently to the surface modified with hetero-bifunctional self-assembled monolayers of alkylthiols (11). However, these approaches produce a layer of randomly oriented antibody molecules on the cantilever surface, thereby generating conformational heterogeneity and inactive receptor molecules (13,14).As previously shown (13,(15)(16)(17)(18), the sensitivity of immunosensors can be improved by both maximizing the degree of functional ...

show abstract

Section: Discussionmentioning

confidence: 99%

A label-free immunosensor array using single-chain antibody fragments

Backmann

Zahnd

Huber

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

266

183

View full text Add to dashboard Cite

show abstract

“…The use of wax for the creation of microfluidic devices has been used to create paper and glass-based devices as a simple and inexpensive method using commercially available materials [23][24][25] . This ability to create biosensors has been investigated to an extent with inkjet printing technology [26][27][28][29] . In this paper we have described for first time inkjet printing paraffin wax on tissue culture plastic and on a plain glass substrate and these formed structures were then used to guide cell attachment, spreading and proliferation, without further processing steps.…”

Section: Introductionmentioning

confidence: 99%

Utilising inkjet printed paraffin wax for cell patterning applications

Tse

Stringer

et al. 2024

IJB

View full text Add to dashboard Cite

Abstract:We describe a method to prepare patterned environments for eukaryotic cells by inkjet printing paraffin wax onto a substrate. This technique bypasses the requirement to create a master mould, typically required with the use of polydimethylsiloxane techniques and the printed structure could be immediately used to guide cell proliferation. In a space of 2-3 hours, the desired pattern could be created with computer assisted design, printed and have cells seeded onto the scaffold, which could reduce the cycle time of prototyping micropattern designs. Human dermal fibroblasts and RN22 Schwann cells were seen to proliferate within the fabricated patterns and survive for more than 7 days. Additionally, the wax constructs could be readily removed from the substrate at any stage after cell seeding with the cells continuing to proliferate. Thus, we report on a simple but novel approach for the controlled physical positioning of live cells by wax inkjet printing.

show abstract

“…A microfluidic chamber was assembled with the electrode chip after printing silver (Ag) 3D electrodes for protecting particles and adjusting for fluid control. The DEP performance was verified using the 4 μm polystyrene beads and AC drive voltage control [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a 3 dimensional dielectrophoresis electrode by a metal inkjet printing method

Lee

Yun

Koh

et al. 2013

Micro Nano Syst Lett

View full text Add to dashboard Cite

We proposed a micro electrode fabrication method by a metal inkjet printing technology for the bio-applications of dielectrophoresis (DEP). The electrodes are composed of bottom planar gold (Au) electrodes and three dimensional (3D) silver (Ag) electrodes fabricated locally on the Au electrode through metal inkjet printing. We observed the negative DEP characteristics of the 4 μm polystyrene beads on the both electrodes at the 500 kHz, AC 20 V pp point. The number of beads trapped on the printed Ag electrode is 79 and 25 on the planar Au electrode because of spatially larger electric field in a 3D electrode system.

show abstract

Rapid functionalization of cantilever array sensors by inkjet printing

Cited by 222 publications

References 27 publications

A label-free immunosensor array using single-chain antibody fragments

A label-free immunosensor array using single-chain antibody fragments

Utilising inkjet printed paraffin wax for cell patterning applications

Fabrication of a 3 dimensional dielectrophoresis electrode by a metal inkjet printing method

Contact Info

Product

Resources

About