Rapid generation of three‐dimensional microchannels for vascularization using a subtractive printing technique

Burtch, Stephanie Renée; Sameti, Mahyar; Olmstead, Richard; Bashur, Chris A.

doi:10.1002/jbio.201700226

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…The meshes were prepared by electrospinning solutions of pure PCL and collagen type I as well as 10%, 25%, and 50% blends of collagen/PCL (w/w) dissolved in HFIP as described in the previous studies. [ 45 ] Corresponding gelatin type A and gelatin/PCL control conditions were also electrospun. For testing platelet response, these meshes were electrospun directly on the drum as sheets, and for characterization of the biomaterial mechanism, they were electrospun onto coverslips.…”

Section: Methodsmentioning

confidence: 99%

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Sameti

Clarke

Dewan

et al. 2021

Macromolecular Bioscience

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A clinically approved, tissue engineered graft is needed as an alternative for small‐diameter artery replacement. Collagen type I is commonly investigated for naturally derived grafts. However, collagen promotes thrombosis, currently requiring a graft pre‐seeding step. This study investigates unique impacts of blending low collagen amounts with synthetic polymers on scaffold platelet response, which would allow for viable acellular grafts that can endothelialize in vivo. While platelet adhesion and activation are confirmed to be high with 50% collagen samples, low collagen ratios surprisingly exhibit the opposite, anti‐thrombogenic effect. Different platelet interactions in these blended materials can be related to collagen structure. Low collagen ratios show homogenous distribution of the components within individual fibers. Importantly, blended collagen scaffolds exhibit significant differences from gelatin scaffolds, including retaining percentage of collagen after incubation. These findings correlate with functional benefits including better endothelial cell spreading on collagen versus gelatin blended materials. This appears to differ from the current paradigm that processing with harsh solvents will irreversibly denature collagen into less desirable gelatin, but an important distinction is the interaction between collagen and synthetic materials during processing. Overall, excellent anti‐thrombogenic properties of low collagen blends and benefits after grafting show promise for this vascular graft strategy.

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Section: Methodsmentioning

confidence: 99%

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Sameti

Clarke

Dewan

et al. 2021

Macromolecular Bioscience

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“…printing process using a photodisruption technique. 4 However, there is no template channel approach that is currently in clinical trials.…”

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confidence: 99%

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Somasekhar

Huynh

Vecheck

et al. 2021

J Biomedical Materials Res

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Hydrogels such as alginate and gelatin have shown potential as biomaterials in various three-dimensional (3D) bioprinting applications. However, parameters such as viscosity, porosity, and printability influence the performance of hydrogel-based biomaterials, and there are limited characterization studies conducted on the behavior of these constructs. In this work, a syringe-based extrusion bioprinter was used to print 3D constructs with bioink composed of various concentrations of alginate and gelatin along with fibrinogen and human umbilical vein endothelial cells. Instead of crosslinking the gelatin, the gelatin was left uncrosslinked to provide microporosity within the system that can impact the cellular response. Mechanical and biochemical characterization was performed to evaluate the structural stability and integrity of the printed constructs along with viability of embedded cells. Bioprinted constructs of a higher total concentration of alginate and gelatin yielded better stability and structural integrity after culture. More importantly, higher amounts of gelatin (i.e., 1:9 instead of 2:3 alginate:gelatin) were shown to improve printability, which is different than most studies that instead use alginate to improve printability. In addition, higher amounts of gelatin impacted the changes in surface morphological features of the constructs after incubation, and ultimately improved biocompatibility with our system. Overall, this study demonstrated that an uncrosslinked gelatin system can provide flexible printing parameters and surface morphologies, but careful control over the printing parameters may be required. The bioink concentration of 10% (w/v) with minimum alginate and higher gelatin concentration exhibited the best printability, cell survival, and viability.

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“…The process of angiogenesis in vivo is typically divided into three stage, i.e. proliferation, invasion, and maturation [46,47]. Accordingly, after injection of the microrods, local irritations such as inflammations can be induced in the tissues, which accumulate at the site of action and induce angiogenesis.…”

Section: In Vivo Investigationsmentioning

confidence: 99%

Endothelialized microrods for minimally invasive in situ neovascularization

Wang

Kankala

et al. 2019

Biofabrication

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Despite the significant advancements in fabricating various scaffolding systems over the past decades, generation of functional tissues towards vascularization remains challenging for the currently available biofabrication approaches. On the other hand, the applicability of traditional surgical transplantation of vascularized tissue constructs is sometimes limited due to the sophisticated surgical procedures, which are invasive, leading to increased risks of scar formation and infection. Considering these facts, we present an innovative platform, the angiogenic microrods composed of sodium alginate/gelatin harboring proliferating endothelial cells using a specially designed double T-junction microfluidic device with an expansion chamber, for achieving minimally invasive neovascularization in situ. Such vessel-like microarchitectures could be derived through controlled penetration of the crosslinker genepin for the gelatin phase, ensuing differential degrees in crosslinking of peripheral and central portions of the microstructures, thus resulting in the formation of vascular lumen-like hollow cavities via endothelial cell migration and proliferation during culture in vitro. Furthermore, in vivo performance of the microrods was explored. We believe that the development of these modular microrods for minimally invasive delivery is of great interest and offers a convenient approach for vascularization in situ.

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Rapid generation of three‐dimensional microchannels for vascularization using a subtractive printing technique

Cited by 6 publications

References 47 publications

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Endothelialized microrods for minimally invasive in situ neovascularization

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