Rapid genotyping of Achromobacter xylosoxidans, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates using melting curve analysis of RAPD-generated DNA fragments (McRAPD)

Deschaght, Pieter; Simaey, Leen Van; Decat, Ellen; Mechelen, Els Van; Brisse, Sylvain; Vaneechoutte, Mario

doi:10.1016/j.resmic.2011.02.002

Cited by 27 publications

(39 citation statements)

References 22 publications

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“…A relatively new method, melting-curve analysis of RAPDgenerated DNA fragments (McRAPD), has shown promise for the analysis of small numbers of S. maltophilia isolates at a time (86). The method demonstrated a sensitivity comparable to that of RAPD analysis followed by agarose gel electrophoresis in its ability to discriminate between S. maltophilia isolates and group them into genotypes.…”

Section: Comparing Clinical and Environmental S Maltophilia Isolatesmentioning

confidence: 99%

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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Section: Comparing Clinical and Environmental S Maltophilia Isolatesmentioning

confidence: 99%

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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“…to our previous experience. Melting analysis of RAPD, termed McRAPD, was first used by our group for purposes of yeast species identification 19 , but later proved to be useful for strain typing in several other settings 17,[20][21][22] . In this study, McRAPD provided excellent results that were in full accordance with the results of MLST, which is considered the gold standard for microbial typing.…”

Section: Maldi-tof Ms Strain Typingmentioning

confidence: 99%

McRAPD unlike MALDI-TOF MS is a suitable candidate for routine discrimination of new Haemophilus influenzae strain acquisition in chronic obstructive pulmonary disease (COPD) and cystic fibrosis

Raclavsky¹,

Stromerova

Šafářová

et al. 2016

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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a Background and Aims. Haemophilus influenzae new strain acquisition has been demonstrated to increase the relative risk of acute exacerbation fourfold in contrast to colonisation or chronic infection by the same strain in chronic obstructive pulmonary disease (COPD). Unfortunately, molecular typing techniques are not suitable for routine use due to cost, labour-intensity and need for special expertise. We tested two techniques potentially useful for routine typing, namely the newly available MALDI-TOF MS and the modified McRAPD compared to MLST as the gold standard. Methods. In 10 patients (10.8%) suffering from COPD or cystic fibrosis, H. influenzae isolates were recovered repeatedly at different timepoints from the same patient during the study period. This allowed for thirteen pairwise comparisons of typing results in isolates recovered consecutively from the same patient to test the ability of the techniques to uncover new strain acquisition. Results. MLST detected 9 cases of new strain acquisition among the 13 pairwise comparisons. However, MALDI-TOF MS reported all 13 pairs as different and thus new. In contrast, McRAPD was able to differentiate all the new strain acquisitions from pre-existing ones, both by visual inspection of melting profiles and by Relative Significant Difference values. Conclusions. Unlike MALDI-TOF MS, McRAPD appears to be a suitable candidate for routine discrimination of new strain acquisitions because of its accuracy and, rapid, easy and economic performance.

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“…Recently, a melting curve analysis was performed for the amplified DNA fragments that were generated during RAPD. This approach does not require electrophoresis and ethidium bromide by realtime PCR, thus greatly reducing workload and overall time [37]. RAPD has many advantages: it requires low-cost primers, only a small quantity of DNA for analysis, and it requires no blotting or hybridization.…”

Section: Rapid Amplification Of Polymorphic Dna (Rapd)mentioning

confidence: 99%

Genotyping methods for monitoring the epidemic evolution of A. baumannii strains

Ahmed

Alp

2015

J Infect Dev Ctries

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Acinetobacter baumannii is clustered with other phenotypically similar species into what has commonly become known as the ACB complex: A. calcoaceticus, A. pittii and, A. nosocomialis. The ecology and pathology of most of these species are not well understood, mainly because current specific phenotypic techniques have, to date, been insufficient. This has inhibited both the precise identification of, as well as the ability to discriminate between, these clinically important and closely related Acinetobacter strains. However, new genotypic methods have greatly enhanced our capacity to identify the ACB complex. This has resulted in the implementation of more rational infection control programs. Several genotypic identification methods are explored in this study, including non-polymerase chain reaction (PCR)-based and PCR-based methods. These methods include ribotyping, pulsed-field gel electrophoresis, 16S rRNA identification, multilocus sequence typing, single locus sequence typing, restriction fragment length polymorphism analysis, restriction analysis of 16S-23S rRNA intergenic spacer sequences, rapid amplification of polymorphic DNA, and repetitive extragenic palindromic PCR; however, there is no current single ideal genotyping method. Each one has its own advantages and disadvantages. With this in mind we reviewed current and new genotyping methods used to characterize the Acinetobacter species.

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Rapid genotyping of Achromobacter xylosoxidans, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates using melting curve analysis of RAPD-generated DNA fragments (McRAPD)

Cited by 27 publications

References 22 publications

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

McRAPD unlike MALDI-TOF MS is a suitable candidate for routine discrimination of new Haemophilus influenzae strain acquisition in chronic obstructive pulmonary disease (COPD) and cystic fibrosis

Genotyping methods for monitoring the epidemic evolution of A. baumannii strains

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