Rapid Genotyping of <italic>MSTN</italic> Gene Polymorphism Using High-resolution Melting for Association Study in Rabbits

Abstract

The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at −125 bp (relative to ATG start codon) of 5’regulatory region of rabbit MSTN was identified by direct sequencing. Two hundred and twenty two rabbits, which were randomly sampled from 3 breeds (Ira rabbits, Champagne rabbits and Tianfu black rabbits), were genotyped by high-resolution melting (HRM). Comparing the genotyping results of 47 samples with direct sequencing, the HRM showed high sensitivity (0.96) and high specificity (0.98). In the three rabbit breeds, the allele C was the predominant allele. The polymorphic site showed high heterozygosity (He = 0.48) and high effective number of alleles (Ne = 1.91). The genetic diversity was reasonably informative (0.25<PIC<0.50). The association analysis showed that the genotype TC had significant effect on the 84-d-weight of rabbits compared with genotype CC (p = 0.047). In contrast, the genotypes had no significant effect on other production traits. These results showed that HRM could be effectively used for genotyping analysis of MSTN gene. The T/C variant in 5’regulatory region of MSTN might be one of the candidate SNP loci affecting the trait of 84-d-weight.

Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

“…Kurkute et al (2011) rescreened all 3 exons of the MSTN gene and found 2 mutations in exon 1 (c.1A>G) and exon 3 (c.799G>A). Peng et al (2013) identified a T/C variant at -125 bp (relative to ATG start codon) in the rabbit MSTN gene, and, more recently, Sternstein et al (2014) identified 2 novel SNPs (C.-125T>C) and (c. 373+234G>A) and confirmed one known SNP (c. 747+34C>T).…”

La génomique du lapin : avancées, applications et perspectives

“…Fontanesi et al (2011Fontanesi et al ( , 2012a, Fontanesi et al (2012bFontanesi et al ( , 2014 et Fontanesi et al (2016) ont étudié le caractère de croissance le plus couramment enregistré et sélectionné dans les populations commerciales de production de viande, le poids à 70 jours. D'autres auteurs (Peng et al, 2013 ;Zhang et al, 2013aZhang et al, , 2013cLiu et al, 2014 ;Wang et al, 2015) ont étudié des caractères de croissance, de carcasse et de qualité de viande mesurés à des âges différents. Le gène de la myostatine (MSTN) a fait l'objet de nombreuses études chez le lapin en raison des effets bien connus des mutations de ce gène sur le développement du tissu musculaire.…”

“…Les effets de l'hypermuscularité liés aux variations de ce gène ont été décrits chez la souris , le bovin (Grobet et al, 1997 ;Kambadur et al, 1997 ; et le mouton (Clop et al, 2006). Cependant, aucun polymorphisme du gène MSTN affectant les caractères n'a été découvert à ce jour chez le lapin dans les races et lignées étudiées (Fontanesi et al, 2011 ;Peng et al, 2013 ;Sternstein et al, 2014).…”

Identification of Rabbit Myostatin Gene Polymorphisms

“…This enzyme recognized and cut at nucleotides of C|TAG sites. The other method for genotyping of MSTN gene in rabbit was studied by Peng et al (2013).…”