Rapid hepatic perfusion decellularization: technique and critique

Fathi, Ibrahim; Elhammady, Habashi A; Sakr, Mahmoud; Nabawi, Ayman; Marei, Mona K.

doi:10.1111/xen.12212

Cited by 4 publications

(5 citation statements)

References 25 publications

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“…Constant flow pressure rather than constant flow rate was infrequently used [7,8]. Gravity-based perfusion was also reported [9]. An approach of lower SDS concentration with longer perfusion duration or gradually increasing concentration was advocated [10,11], aiming at minimizing the damage to ECM and remnant detergent contents in the decellularized scaffold.…”

Section: Xenotransplantation -New Insightsmentioning

confidence: 99%

“…Studies demonstrated the use of livers from various animal species for decellularization including rats [10,[25][26][27], mice [13], ferrets [28,29], rabbits [9,30,31], sheep [14,21], and swine [8,11,[32][33][34]. Decellularization of whole porcine liver was first reported by Matthiesen et al [2].…”

Section: Xenotransplantation -New Insightsmentioning

confidence: 99%

“…Most decellularization studies include tests for vascular integrity as a part of their characterization panel, including corrosion casting [8,10,25,27], CT angiography [21], fluoroscopy [9,28,29], dye injection, MRI [21], ultrasonography modalities, and confocal microscopy [29], or a combination of them [8,29]. SEM on the other hand is frequently used to demonstrate the vascular architecture and the inter-vascular relations.…”

Section: Vasculaturementioning

confidence: 99%

“…Several studies described a relatively fast decellularization protocols for porcine [18], rabbit [9], and rat livers [25]. The utilization of automated perfusion systems, however, renders the duration of decellularization process a less important parameter, as opposed to the duration required for recellularization and ex vivo prepping.…”

Section: Large-scale Productionmentioning

confidence: 99%

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Whole-Liver Decellularization: Advances and Insights into Current Understanding

Fathi¹,

Eltawila²

2017

Xenotransplantation - New Insights

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Whole-liver decellularization comprises the removal of the antigenic cellular content, while maintaining intact 3D extracellular matrix architecture and the complex native vascular cues. As a result, it challenges the classical hurdles of xenotransplantation and hypothetically allows the production of bioengineered human-size liver constructs. The associated technique and understanding of the determinants of successful application evolved rapidly during the last decade. In this chapter, the authors offer a comprehensive walk-through, starting from the simplicity of the concept to the complexity of clinical application. Avoiding repetition, the chapter covers the fundamentals and advances of decellularization, recellularization, ex vivo perfusion culture, and sterilization techniques. The interplay between the main pivots of whole-liver decellularization, namely intrinsic matrix potentials, immune response, and vasculature is described. An effort was made to dissect the hurdles facing the whole-liver decellularization approach and to highlight the gaps in current literature. The authors also offer insights about some critical concepts including intra-scaffold flow dynamics, gradient zonation, critical cell mass/density, mechano-sensitivity, substrate modifications, nondestructive analysis, and the surgeon's perspective, together with the discussion of published in vivo trials and large-scale production parameters.

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Section: Xenotransplantation -New Insightsmentioning

confidence: 99%

Section: Xenotransplantation -New Insightsmentioning

confidence: 99%

Section: Vasculaturementioning

confidence: 99%

Section: Large-scale Productionmentioning

confidence: 99%

See 2 more Smart Citations

Whole-Liver Decellularization: Advances and Insights into Current Understanding

Fathi¹,

Eltawila²

2017

Xenotransplantation - New Insights

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fathi et al. propose a technique for decellularizing the caudate haptic lobes of rabbits to scaffold to create a transplantable liver. The detergent‐based technique is low‐cost, efficient, and preserved vascular integrity.…”

Section: Basic Sciencementioning

confidence: 99%