Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor

Dittmer, Wendy U.; Evers, Toon H.; Hardeman, W.; Huijnen, W.; Kamps, Rick; Kievit, Peggy de; Neijzen, Jaap H. M.; Nieuwenhuis, Jeroen H.; Sijbers, Mara J.J.; Dekkers, David W. C.; Hefti, Marco H.; Martens, Mike

doi:10.1016/j.cca.2010.03.001

Cited by 95 publications

(43 citation statements)

References 24 publications

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“…The second method consists on the use of the polysaccharidic region located in the Fc region of the antibody and the use of aminated nanoparticles. To our knowledge, few contributions in this line can be found on literature with magnetic microbeads and still less with magnetic nanoparticles [25][26][27][28][29][30][31][32]. The biofunctionalization of magnetic nanoparticles suffers some limitations when compared to surfaces and microbeads, such as easy aggregation with small changes of pH and/or ionic strength, polymeric shell instability, batch to batch MNPs irreproducibility, or presence of surfactants.…”

Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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There is a growing interest in the use of magnetic nanoparticles (MNPs) for their application in quantitative and highly-sensitive biosensors. The use of them as labels of biological recognition events and their detection by means of some magnetic method constitutes a very promising strategy for quantitative high-sensitive lateral-flow assays.In the present article, we report the importance of nanoparticle functionalization for the improvement of sensitivity for a lateral flow immunoassay. More precisely, we have found that immobilization of IgG anti-hCG through its polysaccharide moieties on magnetic nanoparticles allows more successful recognition of the hCG hormone. Although we used the detection of hCG as a model in this work, the strategy of binding antibodies to MNPs through its sugar chains reported here is applicable to other antibodies. Its potential is huge as it will be very useful for the development of quantitative and high-sensitive lateral-flow assays for its use on human and veterinary, medicine, food and beverage manufacturing, pharmaceutical, medical biologics and personal care product production, environmental remediation, etc.

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Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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“…Compared to other filter fabrication methods for plasma extraction such as photolithography (Tachi et al 2009;Ji et al 2008;VanDelinder and Groisman 2006;Xing et al 2008), in situ photopolymerization (Moorthy et al 2004), packed-bead column (Shim et al 2010), and the incorporation of commercially available membranes (Dittmer et al 2010;Gervais and Delamarche 2009), natural deposition of beads is a simple, rapid, low-cost method for the fabrication of the porous blood filter. In this experiment, silica beads 4 lm in diameter (Sphere scientific corporation, China) were prepared for bead slurry in the 1% bovine serum albumin (BSA) and the end concentration of bead slurry was 100% (w/v).…”

Section: Design and Fabrication Of The Dmbp-based Chipmentioning

confidence: 99%

A power-free deposited microbead plug-based microfluidic chip for whole-blood immunoassay

Liu

et al. 2011

Microfluid Nanofluid

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“…70,71,72,73 An optomagnetic biosensor based on nanoparticles that are magnetically actuated and optically detected in a sample fluid has been proposed for troponin detection. 74,75 There are electromagnets placed above and below the cartridge that generate magnetic forces, and supermagnetic particles are controlled and accelerated by them. 75 In 2009, Bruls and coworkers coated the magnetic nanoparticles and the sensor surface with antibody, and magnetic nanoparticles were mixed into sample fluid in the cartridge.…”

Section: Other Optical-based Biosensors For Cardiac Troponin Detectionmentioning

confidence: 99%