Rapid HPLC method for monitoring of lactulose production with a high yield

Rapid HPLC Method for Determination of Isomaltulose in the Presence of Glucose, Sucrose, and Maltodextrins in Dietary Supplements

“…On the other hand, dotted lines (right y -axis) in Figure 2 illustrate a dramatic decrease in peak areas as the water content decreases, perhaps caused by a low ionic strength of the mobile phase, which suggests an adsorption of the analytes on the stationary phase, which was previously observed [ 25 , 26 , 27 ] (adsorption mechanism of separation). Hemström and Irgum [ 56 ] have matched arguments supporting partitioning or adsorption mechanism of HILIC from data in the literature.…”

“…Initial experimental conditions for the method optimization were based on recently published papers on study of monosaccharides and disaccharides retention on a polyol stationary phase [ 25 , 26 , 27 , 55 ], where a diol column (Lichrosphere100 DIOL, Merck, Darmstadt, Germany) [ 25 , 26 ] was mostly applied, but in this paper we used a HALO PentaHILIC column that showed a better performance [ 27 ]. Due to the high affinity towards the stationary phase, a flow rate of 2.0 mL/min could be maintained in all experiments.…”

“…Therefore, a compromise between high retention (high resolution) and sufficient peak area must be chosen. To find better experimental conditions we followed our own experience [ 27 , 55 ]; in the following experiments several ammonium buffers (formate, acetate, bicarbonate) in a mixture with acetonitrile were tested.…”

“…With the ELSD, analytes without UV-vis chromophores can be detected, which is especially important for polar compounds. Therefore, the ELSD is quite often connected with Hydrophilic Interaction Liquid Chromatography mode (HILIC) for analytical applications [ 23 , 24 ], including neutral carbohydrates [ 25 , 26 , 27 ]. Márquez-Sillero et al compared the ELSD with an alternative detector (corona-charged aerosol detector, CAD) for carbohydrate analysis in food [ 24 ].…”

An HPLC tool for process monitoring: rare sugar D- psicose and D- fructose contents during the production through an enzymatic path

“…Because of the position of the thermodynamic equilibrium (Wagner et al, 2015), the enzymatic reactions yield an epimeric mixture of a substrate (D-fructose) and product (D-psicose) (Takeshita et al, 2000;Lee et al, 2006). It is important to monitor the conversion of substrate to the product in the reaction in order to control the process to avoid the process impurities and to optimize the reaction time and also to reduce the process cost (Pazourek, 2019;Kuligowski et al, 2012). Therefore, the development of a routine, sensitive, quick and precise method for the determination of D-psicose content in a reaction mixer is required for the mass production of D-psicose.…”