Rapid Identification and Differentiation of<i>Bartonella</i>Species Using a Single-Step PCR Assay

Jensen, Wayne A.; Fall, Majilinde Z.; Rooney, Jane; Kordick, Dorsey L.; Breitschwerdt, Edward B.

doi:10.1128/jcm.38.5.1717-1722.2000

Cited by 190 publications

(124 citation statements)

References 36 publications

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“…Samples were screened to determine exposure to pathogens using assays performed and interpreted following standardized protocols, as previously described , 2002, Jensen et al 2000, Troyer et al 2005, Franklin et al 2007b, Veir et al 2008, Ruch-Gallie et al 2011. In the majority of cases, pathogen exposure was determined by serological analysis.…”

Section: Pathogen Screeningmentioning

confidence: 99%

Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States

Carver

Bevins

Lappin

et al. 2016

Ecological Applications

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Abstract. Understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. The relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. We screened >1000 samples from sympatric populations of puma (Puma concolor), bobcat (Lynx rufus), and domestic cat (Felis catus) across urban gradients in six sites, representing three regions, in North America for exposure to a representative suite of bacterial, protozoal, and viral pathogens (Bartonella sp., Toxoplasma gondii, feline herpesvirus-1, feline panleukopenea virus, feline calicivirus, and feline immunodeficiency virus). We evaluated prevalence within each species, and examined host trait and land cover determinants of exposure; providing an unprecedented analysis of factors relating to potential for infections in domesticated and wild felids. Prevalence differed among host species (highest for puma and lowest for domestic cat) and was greater for indirectly transmitted pathogens. Sex was inconsistently predictive of exposure to directly transmitted pathogens only, and age infrequently predictive of both direct and indirectly transmitted pathogens. Determinants of pathogen exposure were widely divergent between the wild felid species. For puma, suburban land use predicted increased exposure to Bartonella sp. in southern California, and FHV-1 exposure increased near urban edges in Florida. This may suggest interspecific transmission with domestic cats via flea vectors (California) and direct contact (Florida) around urban boundaries. Bobcats captured near urban areas had increased exposure to T. gondii in Florida, suggesting an urban source of prey. Bobcats captured near urban areas in Colorado and Florida had higher FIV exposure, possibly suggesting increased intraspecific interactions through pile-up of home ranges. Beyond these regional and pathogen specific relationships, proximity to the wildland-urban interface did not generally increase the probability of disease exposure in wild or domestic felids, emphasizing the importance of local ecological determinants. Indeed, pathogen exposure was often negatively associated with the wildland-urban interface for all felids. Our analyses suggest cross-species pathogen transmission events around this interface may be infrequent, but followed by self-sustaining propagation within the new host species.

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Section: Pathogen Screeningmentioning

confidence: 99%

Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States

Carver

Bevins

Lappin

et al. 2016

Ecological Applications

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“…The primers BhCS.781p and BhCS.1137n were used for amplifying the partial fragment (approximately 390 bp) of the gltA gene of Bartonella species (Norman et al, 1995). The PCR-positive samples for the gltA gene were further analyzed by PCR of the 16S/23S rRNA intergenic spacer region (ITS) (Jensen et al, 2000). For further phylogenetic analysis, confirmed Bartonella isolates were analyzed by partial sequences of the ftsZ, rpoB and ribC genes.…”

Section: Dna Extraction and Polymerase Chain Reaction (Pcr) For Confimentioning

confidence: 99%

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Lin

Hsu

Chomel

et al. 2012

Veterinary Microbiology

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Many studies indicated that small mammals are important reservoirs for Bartonella species. Using molecular methods, several studies have documented that bats could harbor Bartonella. This study was conducted to investigate the relationship of Bartonella spp. identified in bats and small mammals living in the same ecological environment. During May 2009 and March 2010, a total of 102 blood specimens were collected. By whole blood culture and molecular identification, a total of 6 bats, 1 rodent and 9 shrews were shown to be infected by Bartonella species. After sequencing and phylogenetic analyses of the sequences of gltA, ftsZ, rpoB and ribC genes, these specific isolates from bats were not similar to the known Bartonella species (the similarity values were less than 91.2%, 90.5%, 88.8%, and 82.2%, respectively); these isolates formed an independent clade away from other known Bartonella type strains. The Bartonella spp. isolated from small mammals, which were closely related to Bartonella tribocorum, Bartonella elizabethae, Bartonella grahamii, Bartonella rattimassiliensis and Bartonella queenslandensis, were similar to the findings in previous studies worldwide. Therefore, the results implied that the species of Bartonella strains isolated from small mammals were different from those identified in bats. Our results strongly suggested that the bat isolate could be a new Bartonella species. This study is also the first one to isolate Bartonella organisms from Asian gray shrews, Crocidura attenuata tanakae.

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“…The samples had been stored at À20(C or À80(C until assayed. Previously published PCR assays for amplification of DNA of Bartonella species (Jensen et al 2000), Ehrlichia species and A phagocytophilum (…”

Section: Assaysmentioning

confidence: 99%

Prevalence of Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’, Bartonella species, Ehrlichia species, and Anaplasma phagocytophilum DNA in the blood of cats with anemia

Ishak

Radecki²,

Lappin

2007

Journal of Feline Medicine and Surgery

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Hemoplasmas are known causes of anemia in some cats and some Bartonella species have been associated with anemia in people and in dogs. In this retrospective study, we used polymerase chain reaction (PCR) assays to determine the prevalence rates of Mycoplasma haemofelis, 'Candidatus M haemominutum', A phagocytophilum, Ehrlichia species, and Bartonella species DNA in the blood of cats with anemia and a control group of healthy cats. DNA of the organisms was amplified from 22 of 89 cats with anemia (24.7%) and 20 of 87 healthy cats (23.0%). DNA of a hemoplasma was amplified from 18 of 89 cats with anemia (20.2%) and 13 of 87 healthy cats (14.9%); DNA of a Bartonella species was amplified from five of 89 cats with anemia (5.6%) and seven of 87 healthy cats (8.0%). There were no statistically significant differences detected between groups.

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Rapid Identification and Differentiation ofBartonellaSpecies Using a Single-Step PCR Assay

Cited by 190 publications

References 36 publications

Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States

Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Prevalence of Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’, Bartonella species, Ehrlichia species, and Anaplasma phagocytophilum DNA in the blood of cats with anemia

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