Rapid identification and global characterization of multiple constituents from the essential oil of Cortex Dictamni based on GC–MS

Abstract

The root of Dictamnus dasycarpus Turcz., also known as Cortex Dictamni, is a Chinese herbal medicine that has been commonly used in the treatment of inflammation, microbial infection, cancer, and other diseases in China for thousands of years. Recently, the essential oil of Cortex Dictamni has been widely studied, and a large number of volatile constituents have been discovered. However, the research of the essential oil of Cortex Dictamni in vivo remains unknown, especially the constituents absorbed into blood after oral administration. Hence, a sensitive and rapid method using gas chromatography with mass spectrometry combined with MassHunter software and the National Institute of Standards and Technology 2014 database was used to investigate the absorbed components in rat serum after oral administration of the essential oil of Cortex Dictamni. With the established method, a total of 36 compounds were screened and identified in the essential oil of Cortex Dictamni based on the mass spectrometry data and compound database. Among them, eight compounds, elemol, thymol methyl ether, β-eudesmol, β-cyclocostunolid, guaiazulene, trans-4-hydroxystilbene, ethyl oleate, and monoelaidin, were tentatively characterized in rat serum. This work demonstrated that the established method proved to be a powerful technique for rapid, simple, reliable, and automated identification of bioactive components of herbal medicine.

8-Methoxyflindersine-Induced Apoptosis and Cell Cycle Disorder Involving MAPK Signaling Activation in Human Colorectal Cancer Cells

“…Several studies have reported that the Dictamni cortex has anti-hepatotoxicity [7], anti-inflammatory [8], and anti-tumor activities [9]. Furthermore, it has been suggested that natural products derived from the Dictamni cortex could be exploited as sources of anticancer drugs [10]. However, its biological activities remain largely unexplored and no substantial evidence demonstrates that natural products derived from the Dictamni cortex [6] can be used for CRC therapy.…”

Rapid discovery and global characterization of multiple components in corn silk using a multivariate data processing approach based on UHPLC coupled with electrospray ionization/quadrupole time‐of‐flight mass spectrometry

“…Based on this method, effective constituents in rat serum after oral administration of many TCM formulas have been clarified in past 20 years . In recent years, especially with the development of LC‐MS approach and multiple data processing technology, serum pharmacochemistry research has obtained in‐depth and comprehensive development in TCM.…”

“…Based on this method, effective constituents in rat serum after oral administration of many TCM formulas have been clarified in past 20 years [1][2][3][4][5][6][7][8]. In recent years, especially with the development of LC-MS approach and multiple data processing technology, serum pharmacochemistry research has obtained in-depth and comprehensive development [9][10][11] in TCM. Corn silk (Zea mays L.) (Figure 1), a well-known abundant herb, has been widely used to anti-diabetes [12,13], antioxidant [14,15], anti-tumor [15][16][17], anti-hypertension [18], anti-inflammation [19,20], anti-fatigue [21], anti-fungal [22], etc.…”

Antitumor Effects and Tumor-specificity of Guaiazulene-3-Carboxylate Derivatives Against Oral Squamous Cell CarcinomaIn Vitro

“…In contrast, much reduced numbers of papers of guaiazulene ( Figure 1B), a lipophilic azulene derivative, have been published, approximately half of which have dealt with its chemistry. Guaiazulenes also have antioxidant and anti-inflammatory activities (9-11) and a few papers have dealt with the treatment of oral diseases (12,13).…”