Rapid Identification of Bacteria with a Disposable Colorimetric Sensing Array

Abstract

Rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar have been achieved from the volatiles they produce using a disposable colorimetric sensor array in a Petri dish imaged with an inexpensive scanner. All ten strains of bacteria tested, including E. faecalis and S. aureus and their antibiotic resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important timeframe. Furthermore, the colorimetric sensor arrays also prove useful as a simple research tool for the study of bacterial metabolism and as an easy method for the optimization of bacterial production of fine chemicals or other fermentation processes.

Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

“…Colorimetric methods are extremely attractive because they offer advantages of low-cost portable instruments and simple operation process; moreover, they can be easily read out with naked eyes (Carey et al, 2011;Laurieri et al, 2010;Li et al, 2011;Lou et al, 2011). Noble metal nanomaterials, in particular, gold nanorods (AuNRs), have attracted much attention in colorimetric detection due to their interesting physical and chemical properties (Castellana et al, 2011;Kuo et al, 2010;Liu et al, 2011).…”

Colorimetric Sensor Array Allows Fast Detection and Simultaneous Identification of Sepsis-Causing Bacteria in Spiked Blood Culture

“…Moreover, the CSA includes rapidly reversible indicators that allow the sensor to track temporal fluctuations in headspace VOCs. When placed in bacterial cultures growing on agar, the CSA can distinguish between bacterial species with 98.8% accuracy (14). In this report, we describe the application of the CSA for early detection and the simultaneous identification of blood cultures inoculated with sepsis-causing bacteria.…”

Using Fluorescence Intensity of Enhanced Green Fluorescent Protein to Quantify Pseudomonas aeruginosa

“…Many different modalities have been established to quantify bacteria in planktonic and biofilm cultures, including direct cell enumeration (plate counting, microscopy, Coulter counting, and flow cytometry) [15][16][17][18][19][20][21][22][23][24][25], optical density [26][27][28], dry mass analysis [29][30][31], protein assays [32][33][34][35][36][37][38], and several staining methods [16,22,23]. The current gold standard method to quantify bacteria is cell enumeration by plate counting, offering multiple advantages.…”