Rapid Identification of Calotropagenin Glycosides Using High‐Performance Liquid Chromatography Electrospray Ionisation Tandem Mass Spectrometry

Abstract

High performance liquid chromatography in combination with electrospray ionization tandem mass spectrometry involving metal cationisation by post column addition of alkali salts was successfully utilised for the rapid identification of calotropagenin glycosides/derivatives in Calotropis procera extract.

Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation

“…The tandem mass spectrum showed daughter ions at m/z 387, 359, 351, 334 and 323 (Fig. 7), which was accordant with the specific MS/MS spectra of calotropagenin (Kanojiya and Madhusudanan 2011). The result suggested that some of cardenolides in C. gigantea hairy root cultures are derivatives of calotropagenin.…”

Pilot Study with regard to the Wound Healing Activity of Protein fromCalotropis procera(Ait.) R. Br.

“…Previously, it was reported that the apical twigs and latex of C. procera produced greatest inhibition zones against S. aureus [32]. Furthermore, we demonstrated the high antimicrobial potency of the C. procera extract against all the tested bacteria may be due to the high content of glycosides and various proteins present in the aqueous extracts [23, 24]. The differences in the antimicrobial activity of various extracts may be directly related to the diversity of compounds accumulated in C. procera : proteins [21], calotropagenin glycosides [23], cardenolides, flavonoids, and saponins [24] thus corroborating our results.…”

“…Furthermore, we demonstrated the high antimicrobial potency of the C. procera extract against all the tested bacteria may be due to the high content of glycosides and various proteins present in the aqueous extracts [23, 24]. The differences in the antimicrobial activity of various extracts may be directly related to the diversity of compounds accumulated in C. procera : proteins [21], calotropagenin glycosides [23], cardenolides, flavonoids, and saponins [24] thus corroborating our results. Such compounds can bind the Gram-negative bacteria to form a heavy soluble complex on the cell surface that subsequently disturbs the interaction between bacteria and cell receptors.…”

“…C. procera latex administered to rats revealed toxic, wound healing, and pain-killing effects [22]. Chemical compounds in the latex are calotropagenin glycosides/derivatives [23], cardenolides, flavonoids, and saponins [24]. However, the C. procera stem bark has hitherto not been well studied completely.…”

Untitled

“…The mobile phase consisted of (A) 1:1 (v/v) acetonitrile: methanol and (B) 0.1mM ammonium acetate in water at a flow rate of 1ml/min and injection volume 20µl. The column effluent was detected at 280 nm with an UV detector 9 .…”