Rapid identification of chemical constituents in Hugan tablets by ultra‐performance liquid chromatography‐quadrupole‐exactive orbitrap mass spectrometry

Wang, Xiaowen; Liu, Huiru; Shu, Lexin; Yao, Yaqi; Xu, Yujuan; Wei, Jinxia; Li, Yubo

doi:10.1002/jssc.202300302

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…In recent years, liquid chromatography-mass spectrometry (LC-MS) has been increasingly used in the analysis of components in traditional Chinese medicine [9]. Moreover, an increasing number of scholars were adopting ultra-high-performance-liquid chromatography quadrupole-orbitrap-mass spectrometry (UHPLC-Q-Orbitrap-MS) to identify and analyze unknown herbal medicines in compound prescriptions [10][11][12]. Jang et al [13] used UHPLC-Q-Orbitrap-MS to qualitatively analyze the main components of Jin Mu Tang, and 23 compounds were identified and characterized.…”

Section: Introductionmentioning

confidence: 99%

Rapid classification and identification of chemical components in three different Zanthoxylum species by ultra‐high‐performance‐liquid chromatography quadrupole‐orbitrap‐mass spectrometry

Shu,

Zhang,

Qiu

et al. 2024

J of Separation Science

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Zanthoxylum, as a medicinal and edible herbal medicine, has a long history and complex chemical composition. There are many varieties of Zanthoxylum, and there are differences in composition between varieties. In this study, a rapid classification and identification method for the main components of Zanthoxylum was established using ultra‐high‐performance‐liquid chromatography quadrupole‐orbitrap‐mass spectrometry. The components of Shandong Zanthoxylum bungeanum, Wudu Zanthoxylum bungeanum, and Zanthoxylum schinifolium were identified by studying the characteristic fragmentations and neutral losses of characteristic components. A total of 48 common components and 24 different components were identified and the fragmentation patterns of the main components, such as flavonoids, alkaloids, and organic acids were summarized. These findings provided a reference for the study of pharmacodynamic substance basis and quality control of different varieties of Zanthoxylum.

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Section: Introductionmentioning

confidence: 99%

Rapid classification and identification of chemical components in three different Zanthoxylum species by ultra‐high‐performance‐liquid chromatography quadrupole‐orbitrap‐mass spectrometry

Shu,

Zhang,

Qiu

et al. 2024

J of Separation Science

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“…This method offers superior resolving power, mass accuracy, and sensitivity, resulting in dependable structural identification [14, 15]. Furthermore, ultra‐high‐performance liquid chromatography‐Q Exactive hybrid quadrupole‐orbitrap high‐resolution mass spectrometry (UHPLC‐Q‐Exactive Orbitrap MS) has been utilized to enhance efficiency, as it is a potent method for detecting and identifying chemical constituents within plant extracts or biological specimens [16–19]. Parent ions and subsequent fragments of constituents are generated via the full‐scan MS approaches.…”

Section: Introductionmentioning

confidence: 99%

In vivo comprehensive metabolite profiling of esculetin and esculin derived from chicory in hyperuricemia rats using ultra‐high‐performance liquid chromatography coupled with quadrupole‐orbitrap high‐resolution mass spectrometry

Lv,

Wang,

Wang

et al. 2023

J of Separation Science

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Chicory, renowned for its multifaceted benefits, houses two vital coumarins, esculetin and esculin, both instrumental in reducing uric acid. This study emphasizes the metabolic pathways of esculetin and esculin under both standard and hyperuricemia conditions. Hyperuricemia was induced in Sprague–Dawley rats using oxonic acid potassium salt (300 mg·kg−1) and a 10% fructose water regimen over 21 days. Leveraging the ultra‐high‐performance liquid chromatography‐Q Exactive hybrid quadrupole‐orbitrap high resolution mass spectrometry, we analyzed the fragmentation behaviors of esculetin and esculin in rat bio‐samples. Post oral‐intake of esculetin or esculin, a notable dip in serum uric acid levels was observed in hyperuricemia rats. The investigation unveiled 24 esculetin metabolites and 14 for esculin. The metabolic pathways of both compounds were hydrolysis, hydroxylation, hydrogenation, dehydroxylation, glucuronidation, sulfation, and methylation. Interestingly, certain metabolites presented variations between standard and hyperuricemia rats, indicating that elevated levels of uric acid may affect enzyme activity linked to these metabolic reactions. This is the first systematic study on comparison of metabolic profiles of esculetin and esculin in both normal and hyperuricemia states, which was helpful to enrich our understanding of the complicated structure–activity relationships between esculin and esculetin and shed light to their action mechanism.

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Quality Comparison Between Two Species of Artemisiae Scopariae Herba Through Qualitative Analysis of HPLC Fingerprint and Quantitative Analysis of 12 Representative Constituents

Wang,

Geng,

et al. 2024

Separation Science Plus

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Artemisiae Scopariae Herba (Chinese name Yinchen), the dried aerial part of Artemisia capillaris Thunb. (A. capillaris) or Artemisia scoparia Waldst. et Kit. (A. scoparia), is one of the most commonly used herbal drugs; the one harvested in the spring is habitually named “Mian Yinchen,” and the one harvested in the autumn is habitually named “Hua Yinchen,” but only the former is used in clinical practice in China. There are significant differences in quality between two species of A. Scopariae Herba and among A. Scopariae Herba from different harvest seasons and different producing areas, but the current comparative studies on the differences in quality of A. Scopariae Herba mainly focus on the latter; there are few comparative studies on the difference in quality between A. capillaris and A. scoparia. In this paper, the HPLC fingerprints of 13 batches of A. capillaris and 7 batches of A. scoparia harvested in spring were established, 21 common peaks in the fingerprints were demarcated, the constituents of these common peaks were identified by quadrupole‐TOF‐MS/MS, the chemical pattern recognition analysis, including hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis, were conducted with the quantified peak areas of common peaks as variables, and the contents of 12 constituents unambiguously confirmed by reference substances in 20 batches of A. Scopariae Herba were determined. The results showed that there exist obvious differences in quality between two species of A. Scopariae Herba, and 3,4‐dicaffeoylquinic acid, 4,5‐dicaffeoylquinic acid, 3,5‐dicaffeoylquinic acid, and chlorogenic acid can be selectively used as markers to distinguish the two species of A. Scopariae Herba.

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Rapid identification of chemical constituents in Hugan tablets by ultra‐performance liquid chromatography‐quadrupole‐exactive orbitrap mass spectrometry

Cited by 6 publications

References 54 publications

Rapid classification and identification of chemical components in three different Zanthoxylum species by ultra‐high‐performance‐liquid chromatography quadrupole‐orbitrap‐mass spectrometry

Rapid classification and identification of chemical components in three different Zanthoxylum species by ultra‐high‐performance‐liquid chromatography quadrupole‐orbitrap‐mass spectrometry

In vivo comprehensive metabolite profiling of esculetin and esculin derived from chicory in hyperuricemia rats using ultra‐high‐performance liquid chromatography coupled with quadrupole‐orbitrap high‐resolution mass spectrometry

Quality Comparison Between Two Species of Artemisiae Scopariae Herba Through Qualitative Analysis of HPLC Fingerprint and Quantitative Analysis of 12 Representative Constituents

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