Rapid Identification of Chemical Constituents in Hericium erinaceus Based on LC-MS/MS Metabolomics

Abstract

Hericium erinaceus is a precious edible and medicinal fungus with high nutritional value. It has many functions, such as enhancing immunity, antitumor antioxidation, antihyperglycemic, antihyperlipidemic, and protecting gastric mucosa. However, there are few researches about the H. erinaceus compounds. In this paper, ultraperformance liquid chromatography tandem high-resolution mass spectrometry (UPLC-Q-exactive-MS/MS) was used to isolate and identify the compounds in H. erinaceus. 102 compounds were identified in H. erinaceus by comparing with standard databases such as MZVault, MZCloud, and BGI Library (self-built standard Library by BGI Co., Ltd), including flavonoids, terpenoids, phenolic acids, phenylpropanoids, steroids, organic acids, and amino acids.

Inflammatory Response and Oxidative Stress as Mechanism of Reducing Hyperuricemia of Gardenia jasminoides‐Poria cocos with Network Pharmacology

“…The mobile phase was 0.1% formic acid-water (liquid A) and 0.1% formic acid-acetonitrile (liquid B) with the sequence as 0–2 min 5% B; 2–22 min 5–95% B; 22–27 min 95% B; 27.1–30 min 5% B. The flow rate was 0.3 mL/min, the column temperature was 40°C, and the injection volume was 5 μ L [ 17 ].…”

“…The MS 2 resolution was 35000, AGC is 2e 5 , the maximum injection time was 50 ms, and the fragmentation energy was set as 20, 40, and 60 eV. Ion source (ESI) parameter settings are as follows: sheath gas flow rate was 40 arb, aux gas flow rate was 10 arb, spray voltage of positive ion mode was 3.80 kV, spray voltage of negative ion mode was 3.20 kV, ion capillary temp was 320 °C, and aux gas heater temp was 350 °C [17].…”

Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS

“…e yield was 43.6%. 50 mg extract of F. velutipes was weighed, and then the sample was managed according to Yang et al [19].…”

“…e mass spectrometry parameters were set according to Yang et al [19]. In brief, 150-1500 and 70,000 were used as the mass range and MS resolution, respectively.…”

Olive mill solid waste induces beneficial mushroom-specialized metabolite diversity: a computational metabolomics study

“…Untargeted metabolomics can provide a “snapshot” of the sample, by identifying and quantifying small molecules metabolites. Many studies have made use of untargeted metabolomics to analyze plant samples, and some have investigated mushrooms 25,26 . To facilitate the analysis and interpretation of the complex datasets obtained from HRLC-MS/MS in untargeted metabolomics, computational metabolomics methods are employed.…”