Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages

Larché, Jérôme; Pouillot, Flavie; Essoh, Christiane; Libisch, Balázs; Străuţ, Monica; Lee, Je Chul; Soler, C.; Lamarca, R.; Gleize, E.; Gabard, Jérôme; Vergnaud, Gilles; Pourcel, Christine

doi:10.1128/aac.01233-12

Cited by 23 publications

(20 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With these increased research efforts, a large number of novel phages have been isolated and characterised to target broad-spectrum antimicrobial resistance of Pseudomonas aeruginosa , Staphylocccus aureus and Burkholderia cepacia complex ( BCC ) (Ackermann, 2007; Larche et al, 2012; Essoh et al, 2013), which are major pathogens in chronically infected cystic fibrosis (CF) patients. In addition, the potential of phages to treat respiratory infections caused by MDR bacteria has recently been demonstrated in animal models (Chhibber et al, 2008; Carmody et al, 2010; Debarbieux et al, 2010; Morello et al, 2011; Alemayehu et al, 2012; Semler et al, 2014; Singla et al, 2015; Cao et al, 2015; Pabary et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders

Leung

Parumasivam

Gao

et al. 2017

International Journal of Pharmaceutics

View full text Add to dashboard Cite

This study aimed to develop inhalable powders containing phages active against antibiotic-resistant Pseudomonas aeruginosa for pulmonary delivery. A Pseudomonas phage, PEV2, was spray dried into powder matrices comprising of trehalose (0–80%), mannitol (0–80%) and L-leucine (20%). The resulting powders were stored at various relative humidity (RH) conditions (0, 22 and 60% RH) at 4 °C. The phage stability and in vitro aerosol performance of the phage powders were examined at the time of production and after 1, 3 and 12 months storage. After spray drying, a total of 1.3 log titer reduction in phage was observed in the formulations containing 40%, 60% and 80% trehalose, whereas 2.4 and 5.1 log reductions were noted in the formulations containing 20% and no trehalose, respectively. No further reduction in titer occurred for powders stored at 0 and 22% RH even after 12 months, except the formulation containing no trehalose. The 60% RH storage condition had a destructive effect such that no viable phages were detected after 3 and 12 months. When aerosolised, the total lung doses for formulations containing 40%, 60% and 80% trehalose were similar (in the order of 105 pfu). The results demonstrated that spray drying is a suitable method to produce stable phage powders for pulmonary delivery. A powder matrix containing ≥ 40% trehalose provided good phage preservation and aerosol performances after storage at 0 and 22 % RH at 4 °C for 12 months.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders

Leung

Parumasivam

Gao

et al. 2017

International Journal of Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…To help combat the rapid dissemination of MDR bacteria, the concept of phages for therapeutic use has been slowly re-introduced to the Western world. Recently, a number of in vitro infection models [14, 15], animal models [16–18] and clinical evidence [11, 12, 19–22] have shown the potential of phage therapy in the clearance of MDR bacteria, suggesting it as a highly effective alternative to traditional antibiotics. In addition to using phages as an independent means to treat antibiotic-resistant bacterial infections, combining phage with traditional antibiotic therapies to enhance treatment outcomes has also been proposed [23, 24].…”

Section: Introductionmentioning

confidence: 99%

Production of Inhalation Phage Powders Using Spray Freeze Drying and Spray Drying Techniques for Treatment of Respiratory Infections

et al. 2016

View full text Add to dashboard Cite

Purpose The potential of aerosol phage therapy for treating lung infections has been demonstrated in animal models and clinical studies. This work compared the performance of two dry powder formation techniques, spray freeze drying (SFD) and spray drying (SD), in producing inhalable phage powders. Method A Pseudomonas podoviridae phage, PEV2, was incorporated into multi-component formulation systems consisting of trehalose, mannitol and L-leucine (F1 = 60:20:20 and F2 = 40:40:20). The phage titer loss after the SFD and SD processes and in vitro aerosol performance of the produced powders were assessed. Results A significant titer loss (~ 2 log) was noted for droplet generation using an ultrasonic nozzle employed in the SFD method, but the conventional two-fluid nozzle used in the SD method was less destructive for the phage (~0.75 log loss). The phage were more vulnerable during the evaporative drying process (~0.75 log further loss) compared with the freeze drying step, which caused negligible phage loss. In vitro aerosol performance showed that the SFD powders (~80% phage recovery) provided better phage protection than the SD powders (~20% phage recovery) during the aerosolization process. Despite this, higher total lung doses were obtained for the SD formulations (SD-F1 = 13.1 ± 1.7 × 104 pfu and SD-F2 = 11.0 ± 1.4 × 104 pfu) than from their counterpart SFD formulations (SFD-F1 = 8.3 ± 1.8 × 104 pfu and SFD-F2 = 2.1 ± 0.3 × 104 pfu). Conclusion Overall, the SD method caused less phage reduction during the powder formation process and the resulted powders achieved better aerosol performance for PEV2.

show abstract

“…php (data not shown). However, isolates EML91, EML138 and EML145 from snakes belonging to MC16 showed MTs having only two alleles different from an MLVA clonal complex that includes isolates from urine, pus and pleural exudate studied in [24]. Thus, the present work led to highlighting new MTs as well as new MCs that only include environmental isolates.…”

Section: Diversity Of Soil and Manure Isolatesmentioning

confidence: 66%

“…No isolates from soil and manure clustered with strains from international collections whatever their origin, nor with snake-related isolates. None of the MTs obtained for the 62 isolates from soil and manure and the 17 isolates from the international collection matched with well-known lineage from a database of 454 clinical isolates of P. aeruginosa MLVA types previously described [24,39,46,48] and publicly available at http://mlva.u-psud.fr/mlvav4/genotyping/index. php (data not shown).…”

Section: Diversity Of Soil and Manure Isolatesmentioning

confidence: 99%

Diversity among strains of Pseudomonas aeruginosa from manure and soil, evaluated by multiple locus variable number tandem repeat analysis and antibiotic resistance profiles

Youenou

Brothier

Nazaret

2014

Research in Microbiology

View full text Add to dashboard Cite

The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. Fifteen described VNTR markers were first selected, and 62 isolates recovered from agricultural and industrial soils in France and Burkina Faso, and from cattle and horse manure, along with 26 snake-related isolates and 17 environmental and clinical isolates from international collections, were genotyped. Following a comparison with previously published 9-marker MLVA schemes, an optimal 13-marker MLVA scheme (MLVA13-Lyon) was identified that was found to be the most efficient, as it showed high typability (90%) and high discriminatory power (0.987). A comparison of MLVA with PFGE for typing of the snake-related isolates confirmed the MLVA13-Lyon scheme to be a robust method for quickly discriminating and inferring genetic relatedness among environmental isolates. The 62 isolates displayed wide diversity, since 41 MLVA types (i.e. MTs) were observed, with 26 MTs clustered in 10 MLVA clonal complexes (MCs). Three and eight MCs were found among soil and manure isolates, respectively. Only one MC contained both soil and manure-borne isolates. No common MC was observed between soil and manure-borne isolates and the snake-related or environmental and clinical isolates. Antibiotic resistance profiles were performed to determine a potential link between resistance properties and the selective pressure that might be present in the various habitats. Except for four soil and manure isolates resistant to ticarcillin and ticarcillin/clavulanic acid and one isolate from a hydrocarbon-contaminated soil resistant to imipenem, all environmental isolates showed wild-type antibiotic profiles.

show abstract

Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages

Cited by 23 publications

References 33 publications

Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders

Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders

Production of Inhalation Phage Powders Using Spray Freeze Drying and Spray Drying Techniques for Treatment of Respiratory Infections

Diversity among strains of Pseudomonas aeruginosa from manure and soil, evaluated by multiple locus variable number tandem repeat analysis and antibiotic resistance profiles

Contact Info

Product

Resources

About