Rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy

Menon, Sarala; Bharadwaj, Renu; Chowdhary, AS; Kaundinya, DV; Palande, DA

doi:10.4103/0255-0857.37334

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…In vitro nuclear magnetic resonance (NMR) used for detecting the specific spectral pattern of the amino acids and other volatile substances released by metabolic processes of the bacteria has been applied as a means of differentiating abscesses from other space-occupying lesions in the brain [64–66]. However, the need for NMR with very high Tesla and an expert analysis and interpretation of the spectral patterns are the limiting factors.…”

Section: Alternative and Advanced Techniques To Improve Detection mentioning

confidence: 99%

Microbiological Spectrum of Brain Abscess at a Tertiary Care Hospital in South India: 24-Year Data and Review

Lakshmi

Umabala

Anuradha

et al. 2011

Pathology Research International

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Intracranial abscesses are life-threatening infections that pose a diagnostic challenge not only to the neurosurgeon but also to the microbiologists. Detailed studies documenting the spectrum of infecting agents involved in brain abscesses are limited from India. Materials and Methods. This is a retrospective analysis of 352 samples from 1987 to 2010 analyzed at a tertiary care hospital in South India from 1987 to 2010, to document the changing trends with time. Results. The age of the patients ranged from 2 to 80 years, a larger number of males being affected. Otogenic infections were the most common cause while cryptogenic abscesses were 20%. Gram stain and culture positivity were 78% each. Gram-positive and negative facultative aerobes and obligate anaerobes were also on the rise. Unusual organisms, like Burkholderia pseudomallei, Salmonella typhi, Nocardia species, Cladosporium bantiana, Fonsecaea pedrosoi, Entamoeba histolytica, and Acanthamoeba were also isolated and/or detected from the brain abscesses aspirate or resected tissue. Summary. New and emerging pathogens associated with brain abscess, especially in immunosuppressed individuals, have renewed the necessity of an early detection, and it will be of great value in appropriate management of patients with brain abscess.

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Section: Alternative and Advanced Techniques To Improve Detection mentioning

confidence: 99%

Microbiological Spectrum of Brain Abscess at a Tertiary Care Hospital in South India: 24-Year Data and Review

Lakshmi

Umabala

Anuradha

et al. 2011

Pathology Research International

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“…[3,206,207]. The utility of nuclear magnetic resonance for the identification of anaerobes, including P. melaninogenica and P. intermedia, was also suggested by preliminary studies by Menon et al [208].…”

Section: Diagnosis Of Prevotella Spp Infectionsmentioning

confidence: 99%

New Insights into Prevotella Diversity and Medical Microbiology

Alauzet¹,

Marchandin

Lozniewski³

2010

Future Microbiol.

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In light of recent studies based on cultivation-independent methods, it appears that the diversity of Prevotella in human microbiota is greater than was previously assumed from cultivation-based studies, and that the implication of these bacteria in several human diseases was unrecognized. While some Prevotella taxa were found during opportunistic infections, changes in Prevotella abundance and diversity were discovered during dysbiosis-associated diseases. As member of the microbiota, Prevotella may also be considered as a reservoir for resistance genes. Greater knowledge on Prevotella diversity, as well as new insights into its pathogenic potential and implication in dysbiosis are expected from the use of human microbe identification microarrays, from whole-genome sequence analyse, and from the NIH Human Microbiome Project data. New approaches, including molecular-based methods, could contribute to improve the diagnosis of Prevotella infections.

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“…It is not fully clear whether friendly microbes in human skin possess the fermentation activity and whether ferments including SCFAs of these microbes have probiotic activities to maintain the homeostasis of the skin microbiome. Reports show that SCFAs produced by fermentation of microorganisms have been detected in pus from a deep-seated abscess, an anaerobic microenvironment in the context of human bacterial infection (Demaerel et al 1994; Gorbach et al 1976; Menon et al 2007). Like a ripening fruit, an acne lesion, particularly a closed comedone or deep-seated abscess in an open comedone, creates an anaerobic microenvironment which facilitates overgrowth of Propionibacterium acnes ( P. acnes ).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis in the human skin microbiome mediates fermentation to inhibit the growth of Propionibacterium acnes: implications of probiotics in acne vulgaris

Wang

Kuo

Shu

et al. 2013

Appl Microbiol Biotechnol

231

199

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Increasing evidence demonstrates that commensal microorganisms in the human skin microbiome help fight pathogens and maintain homeostasis of the microbiome. However, it is unclear how these microorganisms maintain biological balance when one of them overgrows. The overgrowth of Propionibacterium acnes (P. acnes), a commensal skin bacterium, has been associated with the progression of acne vulgaris. Our results demonstrate that skin microorganisms can mediate fermentation of glycerol, which is naturally produced in skin, to enhance their inhibitory effects on P. acnes growth. The skin microorganisms, most of which have been identified as Staphylococcus epidermidis (S. epidermidis), in the microbiome of human fingerprints can ferment glycerol and create inhibition zones to repel a colony of overgrown P. acnes. Succinic acid, one of four short-chain fatty acids (SCFAs) detected in fermented media by nuclear magnetic resonance (NMR) analysis, effectively inhibits the growth of P. acnes in vitro and in vivo. Both intralesional injection and topical application of succinic acid to P. acnes-induced lesions markedly suppress the P. acnes-induced inflammation in mice. We demonstrate for the first time that bacterial members in the skin microbiome can undergo fermentation to rein in the overgrowth of P. acnes. The concept of bacterial interference between P. acnes and S. epidermidis via fermentation can be applied to develop probiotics against acne vulgaris and other skin diseases. In addition, it will open up an entirely new area of study for the biological function of the skin microbiome in promoting human health.

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Rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy

Cited by 6 publications

References 16 publications

Microbiological Spectrum of Brain Abscess at a Tertiary Care Hospital in South India: 24-Year Data and Review

Microbiological Spectrum of Brain Abscess at a Tertiary Care Hospital in South India: 24-Year Data and Review

New Insights into Prevotella Diversity and Medical Microbiology

Staphylococcus epidermidis in the human skin microbiome mediates fermentation to inhibit the growth of Propionibacterium acnes: implications of probiotics in acne vulgaris

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