Rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: Reliability as a guide to patient cohorting

D, Jacobsen; Ackerman, Patricia; Payne, Nathaniel R.

doi:10.1016/0196-6553(91)90042-b

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Direct fluorescent-antigen detection (DFA) has become a common rapid method of detection, comparing favorably with tissue culturing (6,7,9,13). Processing and reading of samples by DFA still requires specialized equipment and staff.…”

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Comparison of the RSV Respi-Strip with Direct Fluorescent-Antigen Detection for Diagnosis of Respiratory Syncytial Virus Infection in Pediatric Patients

et al. 2005

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The RSV Respi-Strip was compared to the SimulFluor respiratory screen for detecting respiratory syncytial virus in nasopharyngeal aspirates from pediatric patients. Of samples tested, 115/239 (49%) were positive by direct fluorescent-antigen detection. The sensitivity and specificity of the RSV Respi-Strip were 92% (95% confidence interval [CI], 86% to 96%) and 98% (95% CI, 94% to 100%), respectively, with a diagnostic efficiency of 95%.Respiratory syncytial virus (RSV) is the most common virus causing respiratory infections in young children, occurring as a winter epidemic annually in temperate climates (11). Direct fluorescent-antigen detection (DFA) has become a common rapid method of detection, comparing favorably with tissue culturing (6,7,9,13). Processing and reading of samples by DFA still requires specialized equipment and staff. As such, it is difficult to provide 24 h per day. Several immunoassay antigen detection kits are available, but most require multiple steps in processing (5,6). Lateral-flow immunochromatographic assays have been developed with fewer steps and reliable results (8,10,12). Coris-BioConcept manufactures an RSV detection kit (RSV Respi-Strip [RSV-RS]) which requires only two reagents (extraction buffer and the immunostrips) and two incubations and makes results available within 25 min. We wished to evaluate whether this assay could be implemented in our regional diagnostic laboratory for the detection of RSV in samples from pediatric patients.All specimens in this study were from pediatric patients (mean age, 16 months; age range, 1 week to 17 years) and were submitted from 26 January to 19 February 2004. Samples were collected by nasopharyngeal aspiration done in emergency departments or pediatric wards. Direct fluorescent-antigen testing for RSV (RSV-DFA) was performed using a SimulFluor respiratory screen DFA test (Chemicon International). The result of this test was selected as the a priori gold standard. All samples were processed according to the manufacturer's instructions with centrifugation and spotting of 30 l of resuspended cells on glass slides prior to inoculation into transport media. The cytospin method was not used. Stained slides were read with a fluorescent microscope and a fluorescein isothiocyanate filter. Epithelial cells with intracellular yellow fluorescence were confirmed as positive with pink fluorescence using a tetramethylrhodamine isothiocyanate filter. Two or more distinctly positive cells/slide were required for a positive test result.RSV-RS testing was performed on the same specimens submitted for DFA testing. The testing technologist was blinded to the DFA testing result. Testing was performed in accordance with the manufacturer's package insert. A 0.25-ml aliquot of nasopharyngeal aspirate was mixed with 0.25 ml of extraction buffer in a 3-ml tube and incubated at room temperature for 10 min. Lateral flow strips were then inserted into the tube and incubated for 15 min prior to reading. The presence of a positive control line with a positive test li...

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Comparison of the RSV Respi-Strip with Direct Fluorescent-Antigen Detection for Diagnosis of Respiratory Syncytial Virus Infection in Pediatric Patients

et al. 2005

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“…(26) 880 örneği içeren çalışmalarında DFA yöntemini hücre kültürü ile karşılaştırmışlar ve sırası ile testin duyarlılık, özgüllük, PPD ve NPD'sini %0.95, %0.91 %0.82 ve %0.98 olarak bulmuş-lardır. Ülkemizde ise Gökalp ve ark.…”

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“…Ancak bu testlerin maliyeti yüksektir ve her merkezde uygun laboratuvar koşulları sağlanamamaktadır. DFA duyarlılığı ve özgüllüğü yüksek, hızlı ve nispeten kolay bir yöntemdir ve RSV hızlı tanısında yerini korumaktadır (10,17,22,26) .…”

Section: Gereç Ve Yöntemunclassified

Çocuklarda Respiratuvar Sinsityal Virüs (RSV) Enfeksiyonlarının Tanısında Hücre Kültürü ve Direkt Floresan Antikor Testi Yöntemlerinin Karşılaştırılması

Sağlık¹,

Mutlu²,

Öngüt³

et al. 2015

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Araştırma ÖZET Amaç: Çocukluk çağında sıklıkla görülen enfeksiyon hastalıklarından biri olan alt solunum yolu enfeksiyonlarının (ASYE) en sık etkeni respiratuvar sinsityal virüs (RSV)'tür. Bu çalışmanın amacı ASYE tanısı alan 0-5 yaş grubundaki çocuklarda RSV'nin hücre kültürü ve direkt floresan antikor (DFA) yöntemleri ile araştırılması ve sonuçların hastaların klinik bulguları ile birlikte değerlendirilmesidir. Gereç ve Yöntem: ASYE tanısı alan 0-5 yaş arasındaki 107 ayaktan (poli-klinik Objective: Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infections (LRTI) in children. This study was aimed to investigate the presence of RSV by cell culture and direct fluorescent antibody (DFA) methods in children with LRTI and to evaluate the results in association with the clinical findings of the patients. Materials and Methods: The study included 162 children with LRTI. The age group of the cases was 0-5 years old, of which 107 were outpatients and 55 were inpatients. Nasopharyngeal swab samples were obtained by flocked swabs and transported to the laboratory within the viral transport medium (Copan Diagnostics, Brescia, Italy). Samples were inoculated into the shell-vial cell culture prepared with A549 cells and presence of RSV were investigated in the fixed cells by fluorescence-labeled monoclonal antibodies (Anti-RSV

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“…This would minimize the nosocomial spread of RSV. 4 Jacobsen and associates 4 found the direct fluorescent antibody assay to be sensitive, specific, and with high positive and negative predictive values.…”

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confidence: 99%

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Rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: Reliability as a guide to patient cohorting

Cited by 6 publications

References 20 publications

Comparison of the RSV Respi-Strip with Direct Fluorescent-Antigen Detection for Diagnosis of Respiratory Syncytial Virus Infection in Pediatric Patients

Comparison of the RSV Respi-Strip with Direct Fluorescent-Antigen Detection for Diagnosis of Respiratory Syncytial Virus Infection in Pediatric Patients

Çocuklarda Respiratuvar Sinsityal Virüs (RSV) Enfeksiyonlarının Tanısında Hücre Kültürü ve Direkt Floresan Antikor Testi Yöntemlerinin Karşılaştırılması

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