Rapid identification of Staphylococcus aureus based on a fluorescence imaging/detection platform that combines loop mediated isothermal amplification assay and the smartphone-based system

Abstract

Food associated diseases pose significant public health threat in the United States. Health risks associated with food-borne pathogens drive the need for constant monitoring of food products. An efficient method that can diagnose food-borne pathogens rapidly will be invaluable and in high demand. In this study, we showed the feasibility of a novel rapid detection platform based on fluorescence imaging/detection that combines a user-friendly, portable loop mediated isothermal amplification (LAMP) reaction device and a smartphone-based detection system. The proposed platform was used to detect Staphylococcus aureus which is one of the most important food-borne pathogen especially dairy products. The complete protocol is quicker; the reaction is performed under isothermal conditions and completed in 1 h or less. Experimental results show that LAMP assays were ten-fold more sensitive than PCR-based detection. The proposed smartphone detection system was able to detect and quantify LAMP assay samples containing three different concentrations of S. aureus from 109 CFU/mL down to 103 CFU/mL. The present proof-of-concept study demonstrated that this platform offers a portable, easy to use method for measuring target pathogens with LAMP amplification.

An electrochemical biosensor for Staphylococcus aureus detection based on a multilevel surface 3D micro/nanostructure

“…The result obtained by this method is reliable, but its strict experimental conditions and complicated procedure make it very time-consuming and non-beneficial to taking subsequent protective or therapeutic measures in time. In recent years, many advances have been involved in overcoming the disadvantages of the traditional method, including polymerase chain reaction (PCR), 4,5 enzyme-linked immunosorbent assay (ELISA), 6,7 loop-mediated isothermal amplification (LAMP), [8][9][10] quartz crystal microbalance (QCM), 11 surface plasmon resonance (SPR), 12 flow cytometry (FC), 13 surface-enhanced Raman spectroscopy (SERS), 14 and photochemistry (PC) [15][16][17] techniques. Although the introduction of the above techniques has significantly benefited S. aureus detection, their cost, automation, and sensitivity are still worthy of further improvement.…”