Rapid identification of the α-glucosidase inhibitory compounds from Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.)

Choi, Se Jin; Kim, Jin Kyu; Jang, Jai Man; Shin, Kuk Hyun; Lim, Soon Sung

doi:10.1007/s10068-012-0129-7

Cited by 15 publications

(6 citation statements)

References 23 publications

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“…Two compounds with m/z 951 were detected at different retention time (Peak 38 and 50 ) which significantly differ in their fragmentation pattern, indicating the presence of isomeric structures which is common with ellagitannins. They were tentatively identified as granatin B and geraniin respectively [33, 34]. …”

Section: Discussionmentioning

confidence: 99%

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr

El-Naa

Zaghloul

et al. 2017

BMC Complement Altern Med

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Background Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders.MethodsThe aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator; tumour necrosis factor (TNF)-α were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performedResultsFifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-α in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression.ConclusionThese results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-α, might be the possible hepatoprotective mechanisms.

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Section: Discussionmentioning

confidence: 99%

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr

El-Naa

Zaghloul

et al. 2017

BMC Complement Altern Med

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“…The α-glucosidase inhibitory activity of the extracts and fractions was determined using a modified procedure reported method with a slight modification [1]. The α-glucosidase activity was measured using the substrate p -nitrophenyl-α- d -glucopyranoside ( p NPG), which is hydrolyzed by α-glucosidase to release the product p -nitrophenol, a colorant that can be monitored at 405 nm.…”

Section: Methodsmentioning

confidence: 99%

“…One of the therapeutic approaches for counteracting hyperglycemia is to block the absorption of glucose by inhibiting the activity of carbohydrate-hydrolyzing enzymes such as α-glucosidase in the digestive organs. Small intestinal α-glucosidases (EC 3.2.1.20) are key enzymes involved in dietary carbohydrate digestion in humans [1]. Inhibitors of these enzymes may be effective in decreasing carbohydrate digestion and glucose absorption to suppress postprandial hyperglycemia [2].…”

Section: Introductionmentioning

confidence: 99%

Xanthium strumarium as an Inhibitor of α-Glucosidase, Protein Tyrosine Phosphatase 1β, Protein Glycation and ABTS+ for Diabetic and Its Complication

et al. 2016

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Phytochemical investigation of the natural products from Xanthium strumarium led to the isolation of fourteen compounds including seven caffeoylquinic acid (CQA) derivatives. The individual compounds were screened for inhibition of α-glucosidase, protein tyrosine phosphatase 1β (PTP1β), advanced glycation end products (AGEs), and ABTS + radical scavenging activity using in vitro assays. Among the isolated compounds, methyl-3,5-di-caffeoyquinic acid exhibited significant inhibitory activity against α-glucosidase (18.42 µM), PTP1β (1.88 µM), AGEs (82.79 µM), and ABTS + (6.03 µM). This effect was marked compared to that of the positive controls (acarbose 584.79 µM, sumarin 5.51 µM, aminoguanidine 1410.00 µM, and trolox 29.72 µM respectively). In addition, 3,5-di-O-CQA (88.14 µM) and protocatechuic acid (32.93 µM) had a considerable inhibitory effect against α-glucosidase and ABTS + . Based on these findings, methyl-3,5-di-caffeoyquinic acid was assumed to be potentially responsible for the anti-diabetic actions of X. strumarium.

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“…α-Glucosidase inhibitory activity was determined using a previously reported method with slight modifications [ 39 ]. The initial concentration of the enzyme solution was 0.015 mg/mL in 0.1 M phosphate buffer (pH 6.9) and the initial concentration of the substrate solution was 1 mM in the same phosphate buffer.…”

Section: Methodsmentioning

confidence: 99%

Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

Quispe

Hwang

Wang

et al. 2017

IJMS

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This study investigates in vitro targets related to diabetes in 30 herbal extracts from Peru, for the first time, using α-glucosidase, aldose reductase (AR) inhibitory assays and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging assays. Among the 30 herbal extracts, Hypericum laricifolium Juss. (HL) was the herb which showed more than 50% inhibition in all assays, presenting 97.2 ± 2.0%, 56.9 ± 5.6%, 81.9 ± 2.5%, and 58.8 ± 4.6% inhibition for the α-glucosidase, AR, DPPH, and ABTS assays, respectively. Finally, six bioactive compounds, namely, protocatechuic acid, chlorogenic acid, caffeic acid, kaempferol 3-O-glucuronide, quercetin, and kaempferol were identified in HL by offline high-performance liquid chromatography (HPLC). Quercetin exhibited the strongest inhibition in all enzyme assays and the strongest antioxidant activity. The results suggest that HL shows great potential for the complementary treatment of diabetes and its complications.

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Rapid identification of the α-glucosidase inhibitory compounds from Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.)

Cited by 15 publications

References 23 publications

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Xanthium strumarium as an Inhibitor of α-Glucosidase, Protein Tyrosine Phosphatase 1β, Protein Glycation and ABTS+ for Diabetic and Its Complication

Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

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