Rapid immuno‐monitoring of inoculant plant growth‐promoting microorganisms

Kecskés, Mihály; Rose, Michael T.; Michel, E.; Lauby, B.; Rakotondrainibe, Malala; Casteriano, Andrea; Palágyi, Attila; Moutouvirin, Anaëlle; Elter, Sébastien; Guillas, Romain; Krishnen, Ganisan; Kennedy, Ivan R.

doi:10.1002/elsc.200800101

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Previously, colony immunoblotting technique was evaluated for the specific detection and enumeration of Citrobacter freundii in sterilized or unsterilized carrier media in commercial products (Kecskés et al, 2009). Today, the sensitivity of microbial identification and enumeration in a sample is improved by next generation sequencing Technique (Abbasian et al, 2018).…”

Section: Techniques' For Strain Identificationmentioning

confidence: 99%

From Isolation of Phosphate Solubilizing Microbes to Their Formulation and Use as Biofertilizers: Status and Needs

Soumaré

Boubekri

Lyamlouli

et al. 2020

Front. Bioeng. Biotechnol.

131

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The production of biofertilizers at industrial level is a bottleneck because bacterial strains are generally developed and managed by research laboratories and not by production units. A seamless transition from laboratory to field application is, therefore necessary. This review provides an overview of the constraints that limiting the application or the implementation of Actinobacteria based biofertilizers especially in agricultural field and suggests solutions to overcome some of these limits. General processes of making and controlling the quality of the inoculum are briefly described. In addition, the paper underlines the opportunity of biofertilizers alone or in combination with chemical fertilizers. This review also, highlights the latest studies (until June 2019) and focuses on P-solubilization microorganisms mainly Actinobacteria. The biotechnology of these bacteria is a glimmer of hope for rock phosphate (RP) bioformulation. Since direct application of RP fertilizer is not always agronomically effective due to its sparse solubility.

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Section: Techniques' For Strain Identificationmentioning

confidence: 99%

From Isolation of Phosphate Solubilizing Microbes to Their Formulation and Use as Biofertilizers: Status and Needs

Soumaré

Boubekri

Lyamlouli

et al. 2020

Front. Bioeng. Biotechnol.

131

View full text Add to dashboard Cite

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Field monitoring of plant-growth-promoting rhizobacteria by colony immunoblotting

et al. 2011

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Inoculant plant-growth-promoting bacteria are emerging as an important component of sustainable agriculture. There is a need to develop inexpensive methods for enumerating these organisms after their application in the field, to better understand their survival and impacts on yields. Immunoblotting is one potential method to measure viable cells, but the high cost of the conventionally used nylon membranes makes this method prohibitive. In this study, less expensive alternative materials such as filter papers, glossy photo papers, and transparencies for the purpose of colony immunoblotting were evaluated and the best substance was chosen for further studies. Whatman filter paper No. 541 combined with a 0.01 mol·L(-1) H(2)SO(4) rinsing step gave similar results to nylon membranes but <20% of the overall cost of the original colony immunoblotting assay. The application of the modified immunoblot method was tested on nonsterile clay soil samples that were spiked with high numbers (>10(7) CFU·g(-1)) of the plant-growth-promoting bacteria Pseudomonas fluorescens , Azospirillum brasilense , or Rhizobium leguminosarum . The modified protocol allowed the identification and recovery of over 50% of the inoculated cells of all three strains, amidst a background of the native soil microflora. Subsequently, the survival of P. fluorescens was successfully monitored for several months after application to field-grown rice at Jerilderie, New South Wales, Australia, thus validating the procedure.

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Rapid immuno‐monitoring of inoculant plant growth‐promoting microorganisms

Cited by 2 publications

References 16 publications

From Isolation of Phosphate Solubilizing Microbes to Their Formulation and Use as Biofertilizers: Status and Needs

From Isolation of Phosphate Solubilizing Microbes to Their Formulation and Use as Biofertilizers: Status and Needs

Field monitoring of plant-growth-promoting rhizobacteria by colony immunoblotting

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