Rapid Immunofluorescence Diagnosis of Acute Hemorrhagic Conjunctivitis Caused by Enterovirus 70

Pal, S. R.; Szücs, G; Melnick, Joseph L.

doi:10.1159/000149369

Cited by 12 publications

(4 citation statements)

References 2 publications

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“…Commonly used diagnosed tests for enterovirus infection are based on virus isolation in cell culture, followed by identification of serotypes with neutralizing antisera, or on serological tests. Although no EV70 strain has been isolated by cell culture method since 1988 [24], a reverse-transcription polymerase chain reaction (RT-PCR) [10], which is more sensitive than immunofluorescence [22], enzyme-linked immunosorbent assay [1], or electron-microscopic methods [32], has been applied to detect epidemic EV70 that could not be isolated by cell culture. We have previously reported that 12 samples out of 27 culture-negative specimens from conjunctival swabs of patients among the population of an AHC epidemic in Okinawa, Japan, in 1994 were positive by RT-PCR [27].…”

Section: Introductionmentioning

confidence: 99%

An epidemic of acute haemorrhagic conjunctivitis caused by enterovirus 70 in Okinawa, Japan, in 1994

Uchio

Yamazaki

Ishikawa³

et al. 1999

Graefe's Arch Clin Exp Ophthalmol

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These findings demonstrate that the clinical features of AHC observed in this study were milder than those reported previously, in contrast to the high transmission rate during an epidemic. Changes in clinical features of AHC, such as a low incidence of subconjunctival haemorrhage and disappearance of neurological complications, might be due to biological transformation of EV70. It should be noted that EV70 is still an important aetiological agent of explosive epidemics of AHC.

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Section: Introductionmentioning

confidence: 99%

An epidemic of acute haemorrhagic conjunctivitis caused by enterovirus 70 in Okinawa, Japan, in 1994

Uchio

Yamazaki

Ishikawa³

et al. 1999

Graefe's Arch Clin Exp Ophthalmol

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“…Given the few specimens available for testing and the high background fluorescence observed, further study is needed to evaluate these MAbs for detecting EV-70 in clinical specimens. Other investigators have reported good success in identifying EV-70 antigens by IFA in eye swab specimens from patients with AHC, using polyclonal antiserum (16,17). The degree of reactivity between the two neutralizing MAbs and a given strain of EV-70 differed with the test system.…”

Section: Resultsmentioning

confidence: 99%

Detection of enterovirus 70 with monoclonal antibodies

Anderson¹,

Hatch²,

Flemister³

et al. 1984

J Clin Microbiol

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To improve the ability to identify enterovirus-70 (EV-70) from patients with acute hemorrhagic conjunctivitis, we developed four monoclonal antibodies (MAbs) to EV-70. We reacted the four MAbs against nine previously characterized strains of EV-70 and heterologous viruses by virus neutralization, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). Two of the MAbs neutralized all nine strains of EV-70 and none of the other enterovirus types tested. Two of the MAbs gave a positive reaction with all nine strains by indirect immunofluorescence, and three reacted with all nine strains by ELISA. None of the MAbs gave a positive reaction with heterologous viruses, including those associated with eye disease, by indirect immunofluorescence or ELISA. The two neutralizing MAbs failed to give a positive reaction with some of the strains of EV-70 by indirect immunofluorescence and ELISA, yet they neutralized these viruses. By ELISA with a polyclonal serum as capture antibody and a mixture of MAbs as detector antibody, we were able to detect from 10(2.2) to 10(5.8) 50% tissue culture infective doses of virus and to type lyophilized isolates of EV-70 sent from Taiwan from which we could not recover infectious virus. By choosing the appropriate MAb, or mixture of MAbs, we could construct a test which had the type specificity and strain sensitivity needed to type isolates of EV-70.

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“…Commonly used serological tests must contend with facility constraints in regions prone to AHC epidemics and the availability of paired sera samples. Specificity restrictions (12,13) and sensitivity limitations (10) have also been demonstrated by researchers with their respective diagnostic assays. The objective of this study was to generate a panel of anti-EV-70 MAbs to analyze serotype divergence and identify specific MAbs recognizing conserved antigenic determinants.…”

Section: Introductionmentioning

confidence: 91%

Neutralizing Monoclonal Antibody Against Enterovirus-70 Reacts with Viral Proteins 1C and ID

Wiley¹,

Brodeur²,

Dimock³

et al. 1990

Viral Immunology

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A library of murine monoclonal antibodies against the prototype Enterovirus-70 (EV-70) strain, J670/71, was made for the purpose of studying the immunologically reactive determinants of the virus. Each of the monoclonal antibodies reacted with several other strains of Enterovirus-70 when tested by immunofluorescence. However, none of these monoclonal antibodies reacted with any other picornavirus tested. It was found that all of the monoclonal antibodies precipitated EV-70 viral proteins 1C and 1D in radio-immunoprecipitation assays. However, only one of these monoclonal antibodies, an IgG3 kappa, was capable of neutralizing the virus.

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Rapid Immunofluorescence Diagnosis of Acute Hemorrhagic Conjunctivitis Caused by Enterovirus 70

Cited by 12 publications

References 2 publications

An epidemic of acute haemorrhagic conjunctivitis caused by enterovirus 70 in Okinawa, Japan, in 1994

An epidemic of acute haemorrhagic conjunctivitis caused by enterovirus 70 in Okinawa, Japan, in 1994

Detection of enterovirus 70 with monoclonal antibodies

Neutralizing Monoclonal Antibody Against Enterovirus-70 Reacts with Viral Proteins 1C and ID

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