Rapid in-plate screening of biotransformation products in single zebrafish embryos

Ribbenstedt, Anton; Benskin, Jonathan P.

doi:10.1039/d1ra01111a

Cited by 1 publication

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BioTPs were identified using a previously developed data analysis workflow. 23 Briefly, features that were matched to bioTP exact masses predicted by CD (see Figure 1 ) were selected for further investigation. Thereafter, a modified version of the previously described filter from step (i) of the metabolomic data processing workflow was applied, which removed features with a signal in the negative control samples ( n = 12).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Toxicometabolomics and Biotransformation Product Elucidation in Single Zebrafish Embryos Exposed to Carbamazepine from Environmentally-Relevant to Morphologically Altering Doses

Ribbenstedt

Posselt

Benskin

2022

Chem. Res. Toxicol.

Self Cite

View full text Add to dashboard Cite

Toxicometabolomics and biotransformation product (bioTP) elucidation were carried out in single zebrafish (ZF) embryos exposed to carbamazepine (CBZ). Exposures were conducted in 96-well plates containing six CBZ concentrations ranging from 0.5 μg/L to 50 mg/L ( n = 12 embryos per dose). In the 50 mg/L dose group, 33% of embryos developed edema during the exposure (120 hpf), while hatching was significantly delayed in three of the lower-dose groups (0.46, 3.85, and 445 μg/L) compared to the control at 48 hpf. Toxicometabolomic analysis together with random forest modeling revealed a total of 80 significantly affected metabolites (22 identified via targeted lipidomics and 58 via nontarget analysis). The wide range of doses enabled the observation of both monotonic and nonmonotonic dose responses in the metabolome, which ultimately produced a unique and comprehensive biochemical picture that aligns with existing knowledge on the mode of action of CBZ. The combination of high dose exposures and apical endpoint assessment in single embryos also enabled hypothesis generation regarding the target organ for the morphologically altering insult. In addition, two CBZ bioTPs were identified without additional exposure experiments. Overall, this work showcases the potential of toxicometabolomics and bioTP determination in single ZF embryos for rapid and comprehensive chemical hazard assessment.

show abstract

Section: Methodsmentioning

confidence: 99%

“…BioTPs were identified using a previously developed data analysis workflow . Briefly, features that were matched to bioTP exact masses predicted by CD (see Figure ) were selected for further investigation.…”

Section: Methodsmentioning

confidence: 99%

Toxicometabolomics and Biotransformation Product Elucidation in Single Zebrafish Embryos Exposed to Carbamazepine from Environmentally-Relevant to Morphologically Altering Doses

Ribbenstedt

Posselt

Benskin

2022

Chem. Res. Toxicol.

Self Cite

View full text Add to dashboard Cite

show abstract

Rapid in-plate screening of biotransformation products in single zebrafish embryos

Abstract: A procedure was developed for rapid screening of xenobiotic biotransformation products (bioTPs) in single zebrafish (ZF; Danio rerio) embryos.

Cited by 1 publication

References 42 publications

Toxicometabolomics and Biotransformation Product Elucidation in Single Zebrafish Embryos Exposed to Carbamazepine from Environmentally-Relevant to Morphologically Altering Doses

Toxicometabolomics and Biotransformation Product Elucidation in Single Zebrafish Embryos Exposed to Carbamazepine from Environmentally-Relevant to Morphologically Altering Doses

Contact Info

Product

Resources

About