Rapid In Vitro Proliferation of Tinospora Cordifolia Callus Biomass from Stem and Leaf with Phytochemical and Antimicrobial Assessment

Gedam, Vidyadhar V.; Punyapwar, Swapnil; Raut, Pranay A.; Chahande, Anup D.

doi:10.1007/978-981-13-2784-1_39

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…A broth microdilution method was employed to determine the minimum inhibitory concentration [27] . The microorganism used in this test was Candida albicans ATCC 10231.…”

Section: Resultsmentioning

confidence: 99%

Enamino Barbiturates as Antiproliferative and Antimicrobial Agents: Studies on Synthesis, Biological Activities, and Molecular Modeling

Shtaiwi,

Abu Sarhan,

Aljaar

et al. 2024

ChemistrySelect

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In the present study, the aimed enamines 3 a–j were synthesized with yield ranging between 65 and 81 % by the reaction of 5‐((dimethylamino)methylene)‐1,3‐dimethylpyrimidine‐2,4,6(1H,3H,5H) ‐trione (2) with different amines at room temperature (25 °C). The structures of all synthesized compounds were characterized using different spectroscopic techniques. Molecular docking studies of the new compounds 3 a–j were carried out with human estrogen receptor (hER) enzyme to evaluate their activities as an effective drug molecules. It was investigated that the compounds 3 b, 3 e, 3 f and 3 j revealed the best binding affinities. All new compounds were evaluated towards antifungal activities, which conceded that the compound 3 e displayed a remarkable antifungal activity compared to the Fluconazole and Nystatin, and its Minimum Inhibitory Concentration (MIC) found to be 0.011 mM, while for Fluconazole the MIC value exhibited at 0.046 mM. Additionally, the toxicity for the new compounds found to be better than tamoxifen on MCF‐7 cells and among these molecules, the compound 3 e displayed the lowest IC50 value.

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“…A broth microdilution method was employed to determine the minimum inhibitory concentration [27] . The microorganism used in this test was Candida albicans ATCC 10231.…”

Section: Resultsmentioning

confidence: 99%

Enamino Barbiturates as Antiproliferative and Antimicrobial Agents: Studies on Synthesis, Biological Activities, and Molecular Modeling

Shtaiwi,

Abu Sarhan,

Aljaar

et al. 2024

ChemistrySelect

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show abstract

“…A broth microdilution method was employed to determine the minimum inhibitory concentration (MIC) [ 32 ]. The microorganisms used in this test were five bacteria ( Escherichia coli , Pseudomonas aeruginosa , Proteus mirabilis , Bacillus spizizenii , and Staphylococcus aureus ).…”

Section: Methodsmentioning

confidence: 99%

Design and Synthesis of Thionated Levofloxacin: Insights into a New Generation of Quinolones with Potential Therapeutic and Analytical Applications

Ibrahim

Abul‐Futouh

Bourghli

et al. 2022

CIMB

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Levofloxacin is a widely used fluoroquinolone in several infectious diseases. The structure–activity relationship of levofloxacin has been studied. However, the effect of changing the carbonyl into thiocarbonyl of levofloxacin has not been investigated up to the date of this report. In this work, levofloxacin structure was slightly modified by making a thionated form (compound 3), which was investigated for its antibacterial activity, biocompatibility, and cytotoxicity, as well as spectroscopic properties. The antibacterial susceptibility testing against five different bacteria showed promising minimum inhibitory concentrations (MICs), particularly against B. spizizenii and E. coli, with an MIC value of 1.9 µM against both bacteria, and 7.8 µM against P. mirabilis. The molecular docking experiment showed similar binding interactions of both levofloxacin and compound 3 with the active site residues of topoisomerase IV. The biocompatibility and cytotoxicity results revealed that compound 3 was more biocompatible with normal cells and more cytotoxic against cancer cells, compared to levofloxacin. Interestingly, compound 3 also showed an excitation profile with a distinctive absorption peak at λmax 404 nm. Overall, our results suggest that the thionation of quinolones may provide a successful approach toward a new generation with enhanced pharmacokinetic and safety profiles and overall activity as potential antibacterial agents.

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Rapid In Vitro Proliferation of Tinospora Cordifolia Callus Biomass from Stem and Leaf with Phytochemical and Antimicrobial Assessment

Cited by 2 publications

References 7 publications

Enamino Barbiturates as Antiproliferative and Antimicrobial Agents: Studies on Synthesis, Biological Activities, and Molecular Modeling

Enamino Barbiturates as Antiproliferative and Antimicrobial Agents: Studies on Synthesis, Biological Activities, and Molecular Modeling

Design and Synthesis of Thionated Levofloxacin: Insights into a New Generation of Quinolones with Potential Therapeutic and Analytical Applications

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