Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba

Mok, Pei-Kieng; Ho, Wei-Seng

doi:10.3923/ajps.2019.153.163

Cited by 15 publications

(14 citation statements)

References 33 publications

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“…The addition of compost into the loamy soil does not contribute to better growth or more successful acclimatization of the plantlets at the acclimatization stage. This study agrees with Mok and Ho (2019), where Jiffy-7 provides a high Manuscript to be reviewed percentage of successful acclimatization. Jiffy-7 pellet is only made of compressed peat moss and coco fibers in the fine net that provides adequate aeration water.…”

Section: In Vitro Propagation and Acclimatizationsupporting

confidence: 90%

“…Among others, it is used to induce flowering, fruiting and prevent fungal infections (Wang et al, 2019). PBZ has many uses in in vitro culture for different plants, such as shoot multiplication medium MS + 5 mgL -1 in Phoenix dactylifera (Awadh et al, 2019), rooting medium 1/2 B5 + 0.1 mgL -1 in N. cadamba (Mok & Ho, 2019) and rooting medium MS + 1.5 mgL -1 in Zygopetalum crinitum (Gimenes et al, 2018).…”

Section: In Vitro Propagation and Acclimatizationmentioning

confidence: 99%

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Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

Liston¹

2021

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Section: In Vitro Propagation and Acclimatizationsupporting

confidence: 90%

Section: In Vitro Propagation and Acclimatizationmentioning

confidence: 99%

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

Liston¹

2021

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“…In vitro technique was used in this study to examine the effects of N. cadamba polyploid plants at different stages of micropropagation following the established tissue culture protocol of N. cadamba ( Mok & Ho, 2019 ). With these advantages, mass phenotyping and characterizing of new plants are now a possible task.…”

Section: Discussionmentioning

confidence: 99%

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Eng

Ling³

2021

PeerJ

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Polyploidization has played a crucial role in plant breeding and crop improvement. However, studies on the polyploidization of tropical tree species are still very scarce in this region. This paper described the in vitro induction and identification of polyploid plants of Neolamarckia cadamba by colchicine treatment. N. cadamba belongs to the Rubiaceae family is a natural tetraploid plant with 44 chromosomes (2n = 4x = 44). Nodal segments were treated with colchicine (0.1%, 0.3% and 0.5%) for 24 h and 48 h before transferring to shoot regeneration medium. Flow cytometry (FCM) and chromosome count were employed to determine the ploidy level and chromosome number of the regenerants, respectively. Of 180 colchicine-treated nodal segments, 39, 14 and 22 were tetraploids, mixoploids and octoploids, respectively. The highest percentage of polyploidization (20% octoploids; 6.7% mixoploids) was observed after treated with 0.3% colchicine for 48 h. The DNA content of tetraploid (4C) and octoploid (8C) was 2.59 ± 0.09 pg and 5.35 ± 0.24 pg, respectively. Mixoploid plants are made up of mixed tetraploid and octoploid cells. Chromosome count confirmed that tetraploid cell has 44 chromosomes and colchicine-induced octoploid cell has 88 chromosomes. Both octoploids and mixoploids grew slower than tetraploids under in vitro conditions. Morphological characterizations showed that mixoploid and octoploid leaves had thicker leaf blades, thicker midrib, bigger stomata size, lower stomata density, higher SPAD value and smaller pith layer than tetraploids. This indicates that polyploidization has changed and resulted in traits that are predicted to increase photosynthetic capacity of N. cadamba. These novel polyploid plants could be valuable resources for advanced N. cadamba breeding programs to produce improved clones for planted forest development.

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“…The root growth was also slower in the rooting medium (Table 2 and Figures 2E-2G). According to Mok and Ho (2019), the rooting medium 1/2 B5 + 0.1 mgL -1 PBZ is an optimum medium in inducing a sufficient number of roots in N. cadamba. The PBZ concentration does not affect growth during the rooting, Manuscript to be reviewed and there is no callus formation on the shoot.…”

Section: In Vitro Propagation and Acclimatizationmentioning

confidence: 99%

Peer Review #1 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.2)"

2021

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Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba

Cited by 15 publications

References 33 publications

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Peer Review #1 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.2)"

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