2017
DOI: 10.1186/s13068-016-0691-y
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Rapid in vivo lipid/carbohydrate quantification of single microalgal cell by Raman spectral imaging to reveal salinity-induced starch-to-lipid shift

Abstract: BackgroundLipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only destructive and tedious, but also energy consuming and environment unfriendly. In this study, we first demonstrate that Raman spectroscopy is a clean, fast, and accurate method to simultaneously quantify the lipid/carbohydrate content and ratio in living microalgal cells.ResultsThe quantification r… Show more

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Cited by 45 publications
(37 citation statements)
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“…A strong case can be made that substrate characterization in biorefinery has emerged as a research field in its own right. Characterization of biorefinery resources has been examined by several state-of-the-art analytical techniques including Fourier transform infrared spectroscopy (FTIR) 2 , fluorescence spectroscopy 3 , HPLC 4 , gas chromatography-mass spectrometry (GC-MS) 5 , NMR spectroscopy 6 , gel permeation chromatography (GPC) 7 , scanning electron microscopy 8 , tunneling electron microscopy 9 , atomic force microscopy 10 , Raman spectrometry 11 , time-of-flight secondary ion mass spectrometry (ToF-SIMS) 12 , and small-angle neutron scattering 13 along with a host of wet-chemistry and biological assays.…”
Section: Introductionmentioning
confidence: 99%
“…A strong case can be made that substrate characterization in biorefinery has emerged as a research field in its own right. Characterization of biorefinery resources has been examined by several state-of-the-art analytical techniques including Fourier transform infrared spectroscopy (FTIR) 2 , fluorescence spectroscopy 3 , HPLC 4 , gas chromatography-mass spectrometry (GC-MS) 5 , NMR spectroscopy 6 , gel permeation chromatography (GPC) 7 , scanning electron microscopy 8 , tunneling electron microscopy 9 , atomic force microscopy 10 , Raman spectrometry 11 , time-of-flight secondary ion mass spectrometry (ToF-SIMS) 12 , and small-angle neutron scattering 13 along with a host of wet-chemistry and biological assays.…”
Section: Introductionmentioning
confidence: 99%
“…They are considered as a prospective source of biofuel production, and much research has been devoted to engineering novel or enhanced synthesis pathways of energy‐rich metabolites, lipids and carbohydrates in algae . Recently, we have reported the application of Raman spectroscopy to algae characterization for a label‐free and non‐destructive evaluation of biofuel production . In our previous study, high fluorescence background from the photosynthesis system as well as the inhomogeneous and dynamic intracellular structures were major limiting factors which degrade the speed and accuracy of the quantification.…”
Section: Resultsmentioning
confidence: 92%
“…GC/MS or LC/MS have excellent sensitivity, molecular specificity, and precision, and represent the current 'gold standard' for the quantification of microalgal cellular composition [101]. However, sample pretreatment makes these methods destructive, time-consuming, environmentally unfriendly, and does not allow for real-time lipid content monitoring.…”
Section: Gas and Liquid Chromatography Coupled To Mass Spectrometrymentioning
confidence: 99%
“…Raman micro-spectroscopy has been applied also to quantify the degree of unsaturation by means of the ratiometric method in six species of Mortierella [144]; in situ analysis of lipids accumulated in Botryococcus sudeticus, Chlamydomonas sp., and Trachydiscus minutus [145]; in vivo lipid and carbohydrate quantification of single Chlamydomonas sp. [101]; TAG accumulation in single Nannochloropsis oceanica cells [146]; and quantitative analysis of lipid unsaturation in immobilized Fistulifera solaris [147]. Urban et al…”
Section: Raman Spectroscopymentioning
confidence: 99%