Rapid Inactivation of Ovalbumin Messenger Ribonucleic Acid after Acute Withdrawal of Estrogen

Palmiter, Richard D.; Carey, N. H.

doi:10.1073/pnas.71.6.2357

Cited by 147 publications

(52 citation statements)

References 20 publications

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“…In most steroid-regulated genes, gene expression is mainly influenced by changing the transcription rate (45), but significant differences in the stability of mRNA such as that of ovalbumin mRNA in the presence of estrogen (46), have been reported. In the present study, the RT-PCR assay demonstrated using the primers specific for ATla-R or ATlb-R gene, that the ATla-R mRNA level is increased by 2.1-fold in the presence of dexamethasone, whereas the AT 1 b-R mRNA level is unchanged by dexamethasone.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Matsubara¹,

Kanasaki²,

Murasawa³

et al. 1994

J. Clin. Invest.

142

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Although both rat cardiac nonmyocytes (mostly fibroblasts) and cardiomyocytes have a functional angiotensin II (AngII) receptor, the regulation mechanism of its subtype expression in the rat heart remains unknown. In this study, by using a binding assay and a competitive reverse-transcriptase polymerase chain reaction, we examined the regulation of AngII types la and lb (ATla-R and AT1b-R) and type 2 receptor (AT2-R) expression in embryonal day 19 (E19) and neonatal (1-d) rat cardiac fibroblasts and cardiomyocytes. The number of AT2-R in E19 fibroblasts was dramatically decreased (from 305 to 41 fmol/mg protein) in 1-d fibroblasts, whereas that of AT1-R and the mRNA levels remained unchanged. The ratio of ATla-R to AT1b-R mRNA in both E19 and 1.4 fibroblasts was 9:1. The number of AT2-R in E19 cardiomyocytes was also significantly decreased (from 178 to 87 fmol/mg protein) in 1-d cardiomyocytes, whereas the magnitude was less prominent compared with that in fibroblasts. AT1-R expression remained unaltered in E19 and 1-d cardiomyocytes. In E19 and l-d cardiomyocytes, the ATlb-R mRNA level was 1.5-fold higher than that of AT1a-R mRNA. Dexamethasone induced significant increases in AT1a-R mRNA (2.1-fold) and numbers (1.8-fold) without changing the affinity, whereas neither AT1b-R mRNA nor the number of AT2-R was affected by dexamethasone. The ATla-R gene transcription rate, determined by means of a nuclear run-off assay, was increased (2-fold) by dexamethasone. The half-life of ATla-R mRNA (18 h) was unchanged by dexamethasone. These data indicate that AngI1 receptor subtype expression in the rat heart is regulated in a cell-and subtype-specific manner. (J. Clin. Invest. 1994.

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Section: Discussionmentioning

confidence: 99%

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Matsubara¹,

Kanasaki²,

Murasawa³

et al. 1994

J. Clin. Invest.

142

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“…We recognize that there may be stability features in endogenous mRNAs not present in exogenous reporter mRNAs, which are never as stable as long-lived endogenous mRNAs such as globin (Russell and Liebhaber 1996), ovalbumin (Palmiter and Carey 1974), and casein (Guyette et al 1979). Furthermore, exogenous mRNAs do not go through classical nuclear processing events, although it has been demonstrated that nucleoporation delivers the majority of nucleic acids into the nucleus (Martinet et al 2003;Kurosawa et al 2012).…”

Section: Decay Of a Polyadenylated Reporter Mrna Is Modulated By 59 Amentioning

confidence: 99%

mRNAs containing the histone 3′ stem–loop are degraded primarily by decapping mediated by oligouridylation of the 3′ end

Slepenkov

Slevin

et al. 2012

RNA

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Metazoan replication-dependent histone mRNAs are only present in S-phase, due partly to changes in their stability. These mRNAs end in a unique stem-loop (SL) that is required for both translation and cell-cycle regulation. Previous studies showed that histone mRNA degradation occurs through both 59/39 and 39/59 processes, but the relative contributions are not known. The 39 end of histone mRNA is oligouridylated during its degradation, although it is not known whether this is an essential step. We introduced firefly luciferase reporter mRNAs containing the histone 39 UTR SL (Luc-SL) and either a normal or hDcp2-resistant cap into S-phase HeLa cells. Both mRNAs were translated, and translation initially protected the mRNAs from degradation, but there was a lag of~40 min with the uncleavable cap compared to~8 min for the normal cap before rapid decay. Knockdown of hDcp2 resulted in a similar longer lag for Luc-SL containing a normal cap, indicating that 59/39 decay is important in this system. Inhibition of DNA replication with hydroxyurea accelerated the degradation of Luc-SL. Knockdown of terminal uridyltransferase (TUTase) 4 but not TUTase 3 slowed the decay process, but TUTase 4 knockdown had no effect on destabilization of the mRNA by hydroxyurea. Both Luc-SL and its 59 decay intermediates were oligouridylated. Preventing oligouridylation by 39-deoxyadenosine (cordycepin) addition to the mRNA slowed degradation, in the presence or absence of hydroxyurea, suggesting oligouridylation initiates degradation. The spectrum of oligouridylated fragments suggests the 39/59 degradation machinery stalls during initial degradation, whereupon reuridylation occurs.

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“…This observation is not unique, in that changes in specific mRNA half-life with induction, hormonal changes, or cellular differentiation have been demonstrated in several system (35)(36)(37). In our rat model, uremia seems to influence albumin synthesis by an increased degradation of albumin mRNA by an apparent stimulation of ribonuclease activity.…”

Section: Discussionmentioning

confidence: 72%

Effects of chronic renal failure on protein synthesis and albumin messenger ribonucleic acid in rat liver.

Zern¹,

Yap²,

Strair³

et al. 1984

J. Clin. Invest.

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Abstract. Previously we reported that chronic renal failure in rats leads to preferential disaggregation of liver membrane-bound polysomes associated with a decrease in albumin synthesis. To determine whether reduced albumin synthesis results from reduced cellular levels of albumin messenger RNA (mRNA) or some other molecular mechanism, we have employed mRNA-DNA hybridization in conjunction with cell-free protein synthesis to determine albumin mRNA sequence content and biological activity in subcellular fractions from control and uremic rat liver. Using high specific activity albumin [3H]-complementary DNA prepared from purified-albumin mRNA, we found that total liver polysomes and albumin mRNA sequence content are increased in uremic animals. The extra polysomes are located within the membrane-bound subcellular fraction. These polysomes, however, have reduced ability to synthesize albumin in the cell-free system, and mRNA isolated from membranebound polysomes ofuremic liver showed reduced albumin synthesis. Evaluation of albumin mRNA size by hybridization analysis revealed a reduced content of intact albumin mRNA molecules per microgram of RNA in the A preliminary report of this work has been previously published in abstract form in 1981, Hepatology,

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Rapid Inactivation of Ovalbumin Messenger Ribonucleic Acid after Acute Withdrawal of Estrogen

Cited by 147 publications

References 20 publications

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

mRNAs containing the histone 3′ stem–loop are degraded primarily by decapping mediated by oligouridylation of the 3′ end

Effects of chronic renal failure on protein synthesis and albumin messenger ribonucleic acid in rat liver.

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