Rapid incorporation of glucosinolates as a strategy used by a herbivore to prevent activation by myrosinases

Abdalsamee, Mohamed K.; Giampà, Marco; Niehaus, Karsten; Müller, Caroline

doi:10.1016/j.ibmb.2014.07.002

Cited by 46 publications

(55 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…; Abdalsamee et al. ). The astonishing level of convergence in duplications and specific substitutions observed in insects exposed to cardiac glycosides may be explained by the highly specific interaction between the toxins and a conserved target site (the binding pocket of the Na,K‐ATPase).…”

Section: Discussionmentioning

confidence: 97%

Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants

Dalla¹,

Dobler²

2016

Evolution

View full text Add to dashboard Cite

Herbivorous insects and their adaptations against plant toxins provide striking opportunities to investigate the genetic basis of traits involved in coevolutionary interactions. Target site insensitivity to cardenolides has evolved convergently across six orders of insects, involving identical substitutions in the Na,K-ATPase gene and repeated convergent gene duplications. The large milkweed bug, Oncopeltus fasciatus, has three copies of the Na,K-ATPase α-subunit gene that bear differing numbers of amino acid substitutions in the binding pocket for cardenolides. To analyze the effect of these substitutions on cardenolide resistance and to infer possible trade-offs in gene function, we expressed the cardenolide-sensitive Na,K-ATPase of Drosophila melanogaster in vitro and introduced four distinct combinations of substitutions observed in the three gene copies of O. fasciatus. With an increasing number of substitutions, the sensitivity of the Na,K-ATPase to a standard cardenolide decreased in a stepwise manner. At the same time, the enzyme's overall activity decreased significantly with increasing cardenolide resistance and only the least substituted mimic of the Na,K-ATPase α1C copy maintained activity similar to the wild-type enzyme. Our results suggest that the Na,K-ATPase copies in O. fasciatus have diverged in function, enabling specific adaptations to dietary cardenolides while maintaining the functionality of this critical ion carrier.

show abstract

Section: Discussionmentioning

confidence: 97%

Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants

Dalla¹,

Dobler²

2016

Evolution

View full text Add to dashboard Cite

show abstract

“…larvae revealed the rapid sequestration and concentration of sinalbin in the haemolymph, rather than gut, as a strategy to detoxify ingested leaf material [13], a conclusion likely to be obtained only through MS localization and not through conventional analysis of tissue extracts.…”

Section: Secondary Metabolitesmentioning

confidence: 99%

“…A number of years ago, the Caprioli group pioneered the development of mass spectrometry imaging for localizing molecules of interest directly on the sections of mammalian tissues [2,3]. This methodology, termed matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI, also referred to as imaging mass spectrometry (IMS)), has since been expanded to visualize molecules directly in plant tissues and surfaces for the localization of lipids [4,5,6,7], proteins [8], secondary metabolites [9,10,11,12,13], and various small molecules [14,15,16,17] at unprecedented spatial and chemical resolution.…”

Section: Introductionmentioning

confidence: 99%

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Sturtevant

Lee

Chapman

2016

Current Opinion in Biotechnology

137

View full text Add to dashboard Cite

Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. It is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation Keywords Food biotechnology, Plant biotechnology Disciplines Biotechnology | Chemistry | Plant BiologyComments NOTICE: This is the author's version of a work that was accepted for publication in Current Opinion in Biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Current Opinion in Biotechnology, 37, February 2016, doi: 10.1016/j.copbio.2015 Highlights:Plant chemical organization is visualized directly using MALDI-MSI.MALDI-MS imaging of plant tissues reveals unforeseen analyte compartmentalization.Analyte heterogeneity has been validated by other MS and biochemical analyses.

show abstract

“…The deviation of this pattern in A. rosae may be explained by other advantages of flower feeding, such as an improved defence against predators (see earlier) or unknown factors (Müller & Arand, 2007). The larvae may circumvent the activation of myrosinases by incorporating the GSs very quickly into the haemolymph in the front gut part before myrosinases can become active in successive gut parts (Abdalsamee et al, 2014). The larvae may circumvent the activation of myrosinases by incorporating the GSs very quickly into the haemolymph in the front gut part before myrosinases can become active in successive gut parts (Abdalsamee et al, 2014).…”

Section: Fitness Consequences Of Florivory On Herbivore and On Plantsmentioning

confidence: 97%

Uncovering different parameters influencing florivory in a specialist herbivore

Abdalsamee

Müller

2015

Ecological Entomology

Self Cite

View full text Add to dashboard Cite

1. It has become increasingly recognised that several herbivores switch from folivory (leaf-feeding) to florivory (flower-feeding) during larval development. Yet, it remains poorly understood which cues influence this behaviour, whether a switch to florivory is consistently shown on different hosts, and to what extent florivory could be hindered by plant traits.2. Using the sawfly Athalia rosae and two Brassicaceae differing in architecture and surface structure, the cues that influence larval movement to the flowers were investigated. A broad set of behavioural assays was employed and physical and chemical plant traits potentially affecting the larvae were analysed. Furthermore, the consequences of folivory versus florivory on insect performance were studied.3. The larvae preferred flowers over leaves. Consumption of particular flower parts correlated partly with measured plant traits such as glucosinolate distribution. Visual cues were of higher importance than volatile cues. The initial position of newly hatched larvae on plants influenced the probability of the larvae reaching the flowers during development. Trichomes and surface waxes hindered the larvae from moving upwards to the flowers. Larvae developed slower and gained less body mass when feeding on inflorescences of Brassica nigra than when feeding on leaves, in contrast to the patterns observed before on Sinapis alba, where florivory led to an improved performance.4. This study demonstrates that florivory depends on various host plant traits. It reveals new insights into different parameters influencing this multifaceted phenomenon and into the expected impact on the ecology and fitness of both the attacking herbivores and the plants.

show abstract

Rapid incorporation of glucosinolates as a strategy used by a herbivore to prevent activation by myrosinases

Cited by 46 publications

References 41 publications

Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants

Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Uncovering different parameters influencing florivory in a specialist herbivore

Contact Info

Product

Resources

About