Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Dudani, Jaideep S.; Gossett, Daniel R.; Tse, Henry T. K.; Lamm, Robert J.; Kulkarni, Rajan P.; Carlo, Dino Di

doi:10.1063/1.4907807

Cited by 99 publications

(76 citation statements)

References 27 publications

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“…developed a microfluidic platform based on rapid inertial solution exchange (RInSE) that facilitated continuous-flow, high throughput, and 100% transfer efficiency of exosome capture beads from biofluids into a wash buffer. 77 In the iMER fluidic chip, Shao et al . performed on-chip incubation of the sample with immunomagnetic microbeads that captured a specific subpopulation of exosomes with high yield, facilitating subsequent bead separation using a magnet.…”

Section: Microfluidic-based Exosome Isolationmentioning

confidence: 99%

“…(Figure 5A). 77 Functionalized beads were used for isolation of exosomes, after which they were injected into a microchannel designed to induce inertial focusing and buffer exchange. This approach enabled the beads to be precisely positioned for continuous fluorescence analysis via flow cytometry.…”

Section: Microfluidic-based Exosome Detection and Analysismentioning

confidence: 99%

“…77,80 Dudani et al . used RInSE to isolate capture microbeads from pre-processed blood, with off-chip incubation steps lasting up to 4.5 h (Table 3).…”

Section: Integrated Microfluidic Platforms For Exosome Analysismentioning

confidence: 99%

See 2 more Smart Citations

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

2017

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Exosomes, the smallest sized extracellular vesicles (30 ~ 150 nm) packaged with lipids, proteins, functional messenger RNAs and microRNAs, and double-stranded DNA from their cells of origin, have emerged as key players in intercellular communication. Their presence in bodily fluids, where they protect their cargo from degradation, makes them attractive candidates for clinical application as innovative diagnostic and therapeutic tools. But routine isolation and analysis of high purity exosomes in clinical settings is challenging, with conventional methods facing a number of drawbacks including low yield and/or purity, long processing times, high cost, and difficulties in standardization. Here we review a promising solution, microfluidic-based technologies that have incorporated a host of separation and sensing capabilities for exosome isolation, detection, and analysis, with emphasis on point of care and clinical applications. These new capabilities promise to advance fundamental research while paving the way toward routine exosome-based liquid biopsy for personalized medicine.

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Section: Microfluidic-based Exosome Isolationmentioning

confidence: 99%

Section: Microfluidic-based Exosome Detection and Analysismentioning

confidence: 99%

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Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

2017

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“…The DiCarlo group demonstrated a device that uses the inertial lift force to isolate exosomes from other biological particles. 68 The DiCarlo group fabricated a high aspect ratio micro-channel, which creates an inertial lift force on traveling particles that forces them to the channel centerline. In this device, exosomes were captured onto 20 μm streptavidin microbeads using immunocapture.…”

Section: Introductionmentioning

confidence: 99%

Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices

Carpenter

Issadore

2016

Analyst

187

164

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In the last several years, nanoscale vesicles that originate from tumor cells and which can be found circulating in the blood (i.e. exosomes and microvesicles) have been discovered to contain a wealth of proteomic and genetic information to monitor cancer progression, metastasis, and drug efficacy. However, the use of exosomes and microvesicles as biomarkers to improve patient care has been limited by their small size (30 nm–1 μm) and the extensive sample preparation required for their isolation and measurement. In this Critical Review, we explore the emerging use of micro and nano-technology to isolate and detect exosomes and microvesicles in clinical samples and the application of this technology to the monitoring and diagnosis of cancer.

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“…Therefore, from the recorded electrical signal amplitude, it was possible to determine the relative quantity of presented surface proteins for EVs from different cell lines. Alternatively, Dudani et al [59] used immunological isolation on functionalized beads and size-dependent behavior of particles in microfluidic channels to detect isolated EVs with on-chip flow cytometry in their RInSE (rapid inertial solution exchange) device. Captured 20 µm beads with isolated EVs were focused in a central microfluidic flow while smaller contaminants that were not captured by the functionalized beads did not have sufficient inertial lift to become focused in the centerline of the channel.…”

Section: Immuno-based Microfluidic Devicesmentioning

confidence: 99%

Extracellular Vesicles: Isolation Methods

2017

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Extracellular vesicles (EVs) have recently been at the center of attention of cellular biologists and physicians as their role in intercellular communications has become progressively revealed. EVs display a huge diversity concerning their biogenesis and functions, leading to a still evolving classification comprising exosomes, microvesicles and apoptotic bodies. One of the main technical challenges to studying EVs is to isolate them without interfering with their structure, in order to be able to reveal their functions and to use them as biomarkers. Moreover, the new area of therapeutically using EVs needs clinical grade methods of isolation. In this review, different methods disposable to researchers and clinicians to isolate EVs are described, focusing on the physical principles that allow understanding the advantages and limitations of each technique. The new growing field of microfluidic systems which offers the opportunity to associate isolation and characterization on a single chip will be presented highlighting its potential in the field of EV studies.

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Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Cited by 99 publications

References 27 publications

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices

Extracellular Vesicles: Isolation Methods

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