Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

Abstract

Cabbage is an economically important vegetable worldwide. Cabbage Fusarium Wilt (CFW) is a destructive disease that results in considerable yield and quality losses in cole crops. The use of CFW-resistant varieties is the most effective strategy to mitigate the effects of CFW. 01-20 is an elite cabbage line with desirable traits and a high combining ability, but it is highly susceptible to CFW. To rapidly transfer a CFW resistance gene into 01-20 plants, we used microspore cultures to develop 230 doubled haploid (DH) lines from a cross between 01-20 (highly susceptible) and 96-100 (highly resistant). One of the generated DH lines (i.e., D134) was highly resistant to CFW and exhibited a phenotypic performance that was similar to that of line 01-20. Therefore, D134 was applied as the resistance donor parent. We generated 24 insertion–deletion markers using whole genome resequencing data for lines 01-20 and 96-100 to analyze the genomic backgrounds of backcross (BC) progenies. Based on the CFW resistance gene FOC1, a simple sequence repeat (SSR) marker (i.e., Frg13) was developed for foreground selections. We screened 240 BC1 individuals and 280 BC2 individuals with these markers and assessed their phenotypic performance. The proportion of recurrent parent genome (PRPG) of the best individuals in BC1 and BC2 were 95.8 and 99.1%. Finally, a best individual designated as YR01-20 was identified from 80 BC2F1 individuals, with homozygous FOC1 allele and genomic background and phenotype almost the same as those of 01-20. Our results may provide a rapid and efficient way of improving elite lines through the combined application of microspore culture, whole-genome background analysis, and disease resistance-specific marker selection. Additionally, the cabbage lines developed in this study represent elite materials useful for the breeding of new CFW-resistant cabbage varieties.

iTRAQ-Based Proteomic Analysis of Ogura-CMS Cabbage and Its Maintainer Line

“…Our group developed several elite Ogura-CMS cabbage lines with excellent agronomic performance [ 5 ], and they have been successfully used for the hybrid seed production of many elite varieties. F01-20 is an elite cabbage line originally introduced from Canada [ 28 ], and its Ogura-CMS line CMS01-20 was bred through crosses with a different Ogura-CMS line using F01-20 as the male parent and subsequent recurrent backcrossing with F01-20 for more than 20 generations. The very similar genetic backgrounds of this CMS line and its maintainer line make them ideal materials for cabbage breeding as well as for studying the molecular mechanisms of Ogura-CMS.…”

An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector

“…Cabbage is an important vegetable crop due to its wide adaptability, strong resistance, healthcarerelated value, and favorable taste [44]. Recently, increasing studies of important traits in cabbage have been reported, including petal color, yellow-green leaf, glossy green, hybrid lethality, Fusarium wilt and self-incompatibility [45][46][47][48]. These studies not only enrich the germplasm resources of cabbage but also promote the exploration of the molecular mechanism of cabbage.…”

Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage

“…Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. In crop breeding, introgression populations were used to improve some traits of a crop variety, especially when a variety was found to be sensitive to a specific disease, drought stress or lodging (Dutra et al., ; Liu, Han, et al., ; Liu, Li, et al., ). Introgression populations were also used for pyramiding multiple quantitative trait locus/loci (QTL) in the same background (Liu et al., ; Reinke, Kim, & Kim, ), generating varieties with multiple beneficial alleles.…”