Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy

Agoston, Roland; Izake, Emad L.; Sivanesan, Arumugam; Lott, William B.; Sillence, Martin N.; Steel, Rohan

doi:10.1016/j.nano.2015.11.003

Cited by 35 publications

(16 citation statements)

References 53 publications

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“…All these peaks of different intensities shown in the subtracted spectrum can be assigned to the various biochemical substances and molecular structures according to the reported literatures and previous studies [9, 13–18] (Table 2). Compared with the normal groups, the major increased peaks in the OSCC and MEC groups were assigned to the molecular structures of the nucleic acids and proteins.…”

Section: Resultsmentioning

confidence: 88%

Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma

et al. 2017

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BackgroundOral squamous cell carcinoma (OSCC) is becoming more common across the globe. The prognosis of OSCC is largely dependent on the early detection. But the routine oral cavity examination may delay the diagnosis because the early oral malignant lesions may be clinically indistinguishable from benign or inflammatory diseases. In this study, the new diagnostic method is developed by using the surface enhanced Raman spectroscopy (SERS) to detect the serum samples from the cancer patients.MethodThe blood serum samples were collected from the OSCC patients, MEC patients and the volunteers without OSCC or MEC. Gold nanoparticles(NPs) were then mixed in the serum samples to obtain the high quality SERS spectra. There were totally 135 spectra of OSCC, 90 spectra of mucoepidermoid carcinoma (MEC) and 145 spectra of normal control group, which were captured by SERS successfully. Compared with the normal control group, the Raman spectral differences exhibited in the spectra of OSCC and MEC groups, which were assigned to the nucleic acids, proteins and lipids. Based on these spectral differences and features, the algorithms of principal component analysis(PCA) and linear discriminant analysis (LDA) were employed to analyze and classify the Raman spectra of different groups.ResultsCompared with the normal groups, the major increased peaks in the OSCC and MEC groups were assigned to the molecular structures of the nucleic acids and proteins. And these different major peaks between the OSCC and MEC groups were assigned to the special molecular structures of the carotenoids and lipids. The PCA-LDA results demonstrated that OSCC could be discriminated successfully from the normal control groups with a sensitivity of 80.7% and a specificity of 84.1%. The process of the cross validation proved the results analyzed by PCA-LDA were reliable.ConclusionThe gold NPs were appropriate substances to capture the high-quality SERS spectra of the OSCC, MEC and normal serum samples. The results of this study confirm that SERS combined PCA-LDA had a giant capability to detect and diagnosis OSCC through the serum sample successfully.

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Section: Resultsmentioning

confidence: 88%

Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma

et al. 2017

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“…Meanwhile, the poly(NIPAAm)‐SH aqueous solution changed from cloudy to transparent as we decreased the temperature from 37 to 0 °C (Figure S2d,e, Supporting Information). These phenomena can be ascribed to the significant changes in polarity of poly(NIPAAm) at temperatures below and above its lower critical solution temperature (LCST) of 32 °C …”

Section: Resultsmentioning

confidence: 99%

“…Compared to the direct mixing of HRP with bare gold nanorods and adsorption on nonimprinted gold nanorods, MIP@AuNR incubated with HRP featured a dramatically stronger Raman intensity with prominent peaks of HRP. The characteristic shifts were assigned to the vibrations of S‐S (501 cm −1 ), C‐S (647 cm −1 ), C‐N (1047 cm −1 ), COO − (1393 cm −1 ), CH 2 bending (1443 cm −1 ), amide III (1231 cm −1 ), and aromatic residues of Trp (756, 1030, 1485 cm −1 ), Ala (901, 930 cm −1 ), Phe (1000,1588 cm −1 ), and Tyr (1180 cm −1 ) of HRP (Figure b) . The superiority of MIP@AuNR can be attributed to two reasons: i) the protein‐imprinted SAM on the surface of gold nanorods has a better selectivity and specificity to its template HRP due to the presence of imprinting cavities, and ii) HRP is in close proximity to gold nanorods in the imprinting system and, as a result, benefits more from the local electric field generated by the gold nanorods.…”

Section: Resultsmentioning

confidence: 99%

Targeted Live Cell Raman Imaging and Visualization of Cancer Biomarkers with Thermal‐Stimuli Responsive Imprinted Nanoprobes

Zhang

Qin

Tan

et al. 2018

Part & Part Syst Charact

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Precise visualization of cancer biomarkers within live cells is significantly vital in oncology that requires techniques with high specificity and selectivity. Herein, a new imprinted plasmonic nanoprobe with thermosensitivity is fabricated, which features multifunctional roles as an “artificial antibody” for the active targeting of cancer biomarkers triggered by temperature and can also achieve their in situ Raman detection and imaging within living cells. A thiol‐containing poly(N‐isopropylacrylamide) linear polymer is first synthesized using reversible addition–fragmentation chain transfer polymerization followed by further reduction with NaBH4 to produce free thiol groups at one end. Then, plasmonic nanoprobes are constructed by creating protein‐imprinted poly(N‐isopropylacrylamide) self‐assembled monolayers on the surface of gold nanorods. High specificity and selectivity are demonstrated for the target protein, with an extracellular Raman detection limit of 10−14 mol L−1. Thermally responsive behavior is achieved by modulating the external temperature. The plasmonic properties enable in situ live cell intracellular Raman detection and imaging of endogenous cancer biomarker epidermal growth factor receptor. This technique offers significant promise for visualizing cancer biomarkers and monitoring molecular mechanisms both in vitro and in vivo, which are significant in clinical diagnosis and medical theranostics.

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“…Surface Plasmon Resonance [35,36,37] was thus advanced as an alternative spectroscopic technique to develop an optical biosensor aimed at getting quantitative data about the bacteria recognition, primarily the limit of detection (LOD) of their concentration. As planned, our sensing chips have been fabricated by using aluminum as plasmon-supporting metal instead of the most commonly used gold [38,39] because of the higher affinity of 15 to this substrate. Considerations about the chip preparation conditions are reported in ESI.…”

Section: Resultsmentioning

confidence: 99%

Supramolecular Recognition of Escherichia coli Bacteria by Fluorescent Oligo(Phenyleneethynylene)s with Mannopyranoside Termini Groups

Arias¹,

Méndez²,

Arias³

et al. 2017

Sensors

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Escherichia coli is one the most common bacteria responsible of uropathogenic diseases, which motives the search for rapid and easy methods of detection. By taking advantage of the specific interactions between mannose and type 1 fimbriae, in this work two fluorescent phenyleneethynylene (PE) trimers bearing one or two 4-aminophenyl-α-D-mannopyranoside termini groups were synthesized for the detection of E. coli. Three bacterial strains: ORN 178 (fimbriae I expression), ORN 208 (mutant serotype with no fimbriae expression) and one obtained from a local hospital (SS3) were used. Laser Scanning Confocal Microscopy (LSCM) and Surface Plasmon Resonance (SPR) were applied for the interaction studies following two different approaches: (1) mixing the oligomer solutions with the bacterial suspension, which permitted the observation of stained bacteria and by (2) biosensing as thin films, where bacteria adhered on the surface-functionalized substrate. LSCM allows one to easily visualize that two mannose groups are necessary to have a specific interaction with the fimbriae 1. The sensitivity of SPR assays to E. coli was 104 colony forming unit (CFU)/mL at 50 µL/min flow rate. The combination of PE units with two mannose groups results in a novel molecule that can be used as a specific fluorescent marker as well as a transducer for the detection of E. coli.

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Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy

Cited by 35 publications

References 53 publications

Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma

Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma

Targeted Live Cell Raman Imaging and Visualization of Cancer Biomarkers with Thermal‐Stimuli Responsive Imprinted Nanoprobes

Supramolecular Recognition of Escherichia coli Bacteria by Fluorescent Oligo(Phenyleneethynylene)s with Mannopyranoside Termini Groups

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