“…Every hrHPV strain was amplified during RPA. Cross-reactivity, specificity, and selectivity to potential contaminating pathogens were determined in silico using BLASTn by comparing the primers and probe to common pathogens and human DNA, similar in approach to prior research. , No significant homology to common STIs or other pathogens commonly present in the vaginal canal for both primers was found (Table S2). The analytical LoD was determined by a serial dilution that was performed to identify the lowest concentration that was reproducibly detected.…”