2022
DOI: 10.1038/s41467-022-33546-9
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Rapid joule heating improves vitrification based cryopreservation

Abstract: Cryopreservation by vitrification has far-reaching implications. However, rewarming techniques that are rapid and scalable (both in throughput and biosystem size) for low concentrations of cryoprotective agent (CPA) for reduced toxicity are lacking, limiting the potential for translation. Here, we introduce a joule heating–based platform technology, whereby biosystems are rapidly rewarmed by contact with an electrical conductor that is fed a voltage pulse. We demonstrate successful cryopreservation of three mo… Show more

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Cited by 22 publications
(30 citation statements)
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“…The concentric ring hollow structure was made by using 0.1 mm thickness aluminum film . According to the magnetic field parameters and the way the aluminum film wraps the blood vessel, three solution system rewarming methods are proposed, where P1, P2, and P3 represent the aluminum film at different positions (Figure ).…”
Section: Methodsmentioning
confidence: 99%
“…The concentric ring hollow structure was made by using 0.1 mm thickness aluminum film . According to the magnetic field parameters and the way the aluminum film wraps the blood vessel, three solution system rewarming methods are proposed, where P1, P2, and P3 represent the aluminum film at different positions (Figure ).…”
Section: Methodsmentioning
confidence: 99%
“…(f) Schematic diagram of joule thermal warming. Reproduced with permission from ref . Copyright 2022 Springer Nature.…”
Section: Droplet Warmingmentioning
confidence: 99%
“…Moreover, the external field warming method is also widely used in cryopreservation (Table 4), such as magnetic induction heating (Figure 7e), infrared lamp irradiation, and joule heating technology (Figure 7f). Zhan et al 105 described a platform technique based on Joule heating, in which a biological system was rapidly warmed by contacting an electrical conductor that had been pulsed with an input voltage. The platform technique of Joule heating was predicted to achieve a warming rate of 5 × 10 4 to 6 × 10 8 °C/min.…”
Section: Droplet Warmingmentioning
confidence: 99%
“…In addition, it is impossible to achieve rapid rewarming of large biological samples, and it is challenging to regulate end-point control. Recently, a novel joule heating-based rewarming modality was proposed by Zhan et al 30 This rewarming modality employs tunable voltage pulse widths ranging from 10 μs to 100 ms, which enables the achievement of high warming rates of up to 5 × 10 4 −6 × 10 8 °C/min, as predicted by numerical simulations. The increased rewarming rate offers the advantage of reducing the reliance on the CPA concentration, as a higher critical warming rate is typically required for lower CPA concentrations.…”
Section: Introductionmentioning
confidence: 99%
“…The increased rewarming rate offers the advantage of reducing the reliance on the CPA concentration, as a higher critical warming rate is typically required for lower CPA concentrations. By employing joule heating for rewarming, the demand for CPA concentration in biosystems with varying thicknesses (ranging from 4 μm to 1.2 mm) can be effectively reduced to 2−4 M. 30 This reduction is significant, as it contributes to minimizing CPA toxicity, simplifying the loading and elution procedures, and enhancing the quality of biological samples after cryopreservation.…”
Section: Introductionmentioning
confidence: 99%