Rapid Loss of CD4 T Cells by Pyroptosis During Acute SIV Infection in Rhesus Macaques

Abstract

The mechanisms underlying depletion of CD4 T cells during acute HIV-1 infection are not well understood. Here we show that caspase-1-induced pyroptosis, a highly inflammatory programmed cell death pathway, is the dominant mechanism responsible for the rapid depletion of CD4 T cells in gut-associated lymphatic tissue (GALT), spleen, and lymph nodes during acute simian immunodeficiency virus (SIV) infection in rhesus macaques. Upregulation of interferon-gamma inducible factor 16 (IFI16), a host DNA sensor that triggers pyroptosis, was also observed in tissue-resident CD4 T cells and correlated with viral loads and CD4 T cell loss. In contrast, caspase-3-mediated apoptosis and viral cytotoxicity only accounted for a small fraction of CD4 T cell death. Other programmed cell death mechanisms, including mitochondria-induced caspase-independent cell death, necroptosis, and autophagy, did not significantly contribute to CD4 T cell depletion. These data support a model in which caspase-1-mediated pyroptosis is the principal mechanism that results in CD4 T cell loss in the GALT and lymphoid organs and release of proinflammatory cytokines. These findings contribute to our understanding of the pathogenesis of acute SIV infection and have important implications for the development of therapeutic strategies.

Cardiovascular Disease and Thrombosis in HIV Infection

“…117,121,122 This depletion occurs soon after infection in PLWH and pathogenic SIV (simian immunodeficiency virus) models. 123–125 Depletion of CD4 + T cells persists in patients and nonhuman primate models if the infection is left untreated. Reconstitution of these cells in the gastrointestinal tract depends heavily on immune status and duration of infection before the initiation of cART.…”