Rapid Magnetic Microsphere Enzyme Immunoassay for Potato Virus X and Potato Leafroll Virus

Banttari, E. E.; Clapper, David L.; Hu, S.-P.; Daws, K. M.; Khurana, S. M. P.

doi:10.1094/phyto-81-1039

Cited by 11 publications

(3 citation statements)

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“…In addition to the preservation of different proteins such as enzymes and antibodies, several groups have successfully demonstrated the stabilization and preservation of antibody-based complete immunoassays, i.e., plate-anchored ELISA. − However, to our knowledge, only one group attempted the preservation of a complete, homogeneous, bead-based immunoassay . This attempt, utilizing a commercial stabilization buffer with an unknown composition, was only partially successful, enabling the preservation of the assay components separately but not as a homogeneous assay.…”

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confidence: 99%

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Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents from Complex Matrices

et al. 2016

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This study shows the development of dry, highly stable immunoassays for the detection of bio warfare agents in complex matrices. Thermal stability was achieved by the lyophilization of the complete, homogeneous, bead-based immunoassay in a special stabilizing buffer, resulting in a ready-to-use, simple assay, which exhibited long shelf and high-temperature endurance (up to 1 week at 100 °C). The developed methodology was successfully implemented for the preservation of time-resolved fluorescence, Alexa-fluorophores, and horse radish peroxidase-based bead assays, enabling multiplexed detection. The multiplexed assay was successfully implemented for the detection of Bacillus anthracis, botulinum B, and tularemia in complex matrices.

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“…12−14 However, to our knowledge, only one group attempted the preservation of a complete, homogeneous, bead-based immunoassay. 15 This attempt, utilizing a commercial stabilization buffer with an unknown composition, was only partially successful, enabling the preservation of the assay components separately but not as a homogeneous assay.…”

mentioning

confidence: 99%

Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents from Complex Matrices

et al. 2016

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“…Sensitivity in these assays can be improved, as with antigen-coated wells, by using reporter macromolecules such as biotinylated secondary antibody or biotinylated protein, a conjugate (Thornton, Dewey, & Gilligan 1993). Immunoassays incorporating magnetised beads have been used for some time in the field of medicine and plant virology (Banttari, Calpper, Hu, Daws, & Khurana, 1991) but their use in the diagnosis of fungal plant pathogens is yet to be fully exploited.…”

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confidence: 99%

Application of molecular and immuno-diagnostic tools for detection, surveillance and quarantine regulation of Karnal bunt (Tilletia indica) of wheat

Kumar

Singh

Kumar

et al. 2008

Food and Agricultural Immunology

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Disease diagnostics and pathogen detection, a primary component of any crop management programme, is also helpful in monitoring sanitary and phyto-sanitary measures to seed quality. Molecular diagnostics based on immunological and DNA techniques can provide an efficient way for disease surveillance and disease forecasting. Karnal bunt (KB) of wheat has become a major disease of economic importance and an important issue in the international wheat trade. Due to its seed borne, soil borne and air borne nature, KB can be diagnosed in three pathogenic forms of pathogens viz. teliospores, sporidial and mycelial forms which occur in infectious or proliferative entities at different stages of the disease cycle. Suitable sampling and extraction procedures can improve the detection limits of modern molecular diagnostic methods used to test KB. For the extraction of teliospores from both infected and infested seeds, highly efficacious detergent washing techniques have been developed and extracted pathogenic entities were diagnosed by involving several species of specific primers and immuno-chemical reagents. Nucleic acid and antibody based techniques have been developed for the detection of KB pathogen in different forms. These techniques can also be successfully employed for differential diagnosis, disease surveillance of seed borne pathogens of quarantine importance and determination of teliospore load in wheat seeds. These tests can also be designed to assist in the trade of wheat, development of disease forecasting models and molecular and immuno-pathotyping of Tilletia indica isolates collected from different agro-climatic regions. Moreover, these formats could also be employed for molecular characterization of disease determinants and in comprehending their role in molecular basis of host-pathogen interaction. The present paper highlights the use of the principle of molecular diagnostics in exerting disease surveillance and quality assurances of seeds.

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Potato Viruses and their Management

Khurana

Diseases of Fruits and Vegetables: Volume II

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Rapid Magnetic Microsphere Enzyme Immunoassay for Potato Virus X and Potato Leafroll Virus

Cited by 11 publications

References 0 publications

Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents from Complex Matrices

Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents from Complex Matrices

Application of molecular and immuno-diagnostic tools for detection, surveillance and quarantine regulation of Karnal bunt (Tilletia indica) of wheat

Potato Viruses and their Management

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