Rapid mask prototyping for microfluidics

Maisonneuve, B. G. C.; Honegger, T.; Cordeiro, J.; Lecarme, O.; Thiry, T.; Fuard, D.; Berton, K.; Picard, E.; Zelsmann, M.; Peyrade, D.

doi:10.1063/1.4943124

Cited by 5 publications

(9 citation statements)

References 18 publications

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“…Respective masks for the designed layers were fabricated using a homemade mask-less UV photolithography system 13 , or purchased on transparent films (Selba SA, CH). SU-8 mold fabrication.…”

Section: Methodsmentioning

confidence: 99%

Neurite growth kinetics regulation through hydrostatic pressure in a novel triangle-shaped neurofluidic system

Bgc

Batut²,

Varela³

et al. 2021

Preprint

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Microfluidic neuro-engineering design rules have been widely explored to create in vitro neural networks with the objective to replicate physiologically relevant structures of the brain. Several neurofluidic strategies have been reported to study the connectivity of neurons, either within a population or between two separated populations, through the control of the directionality of their neuronal projections. Yet, the in vitro regulation of the growth kinetics of those projections remains challenging. Here, we describe a new neurofluidic chip with a triangular design that allows the accurate monitoring of neurite growth kinetics in a neuronal culture. This device permits to measure the maximum achievable length of projecting neurites over time and to report variations in neurite length under several conditions. Our results show that, by applying positive or negative hydrostatic pressure to primary rat hippocampal neurons, neurite growth kinetics can be tuned. This work presents a pioneering approach for the precise characterization of neurite length dynamics within an in vitro minimalistic environment.

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Section: Methodsmentioning

confidence: 99%

Neurite growth kinetics regulation through hydrostatic pressure in a novel triangle-shaped neurofluidic system

Bgc

Batut²,

Varela³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Respective masks for the designed layers were fabricated using a homemade mask-less UV photolithography system 13 , or purchased on transparent films (Selba SA, CH).…”

Section: Device Design and Masks Fabrication The Fabricated Microflumentioning

confidence: 99%

“…This procedure was adapted from one of our previously published protocols 13 . The mold was fabricated using a silicon wafer as substrate.…”

Section: Su-8 Mold Fabricationmentioning

confidence: 99%

“…PDMS microfluidic chip fabrication. This procedure was adapted from one of our previously published protocols 13 . The wafer was first silanized using vapours of a silanizing agent (trichloro(1H,1H,2H,2Hperfluorooctyl) silane) in a desiccator for 30 min.…”

Section: Su-8 Mold Fabricationmentioning

confidence: 99%

“…The channels were then rinsed three times with Hank's Balanced Salt Solution ( Neuron preparation and seeding. According to previously published procedures 13 , hippocampi were harvested from E18 OFA rats (Charles River Laboratories) and kept in ice-cold HBSS buffered with 10mM HEPES (pH 7.3). The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.…”

Section: Su-8 Mold Fabricationmentioning

confidence: 99%

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Microchannel patterning strategies forin vitrostructural connectivity modulation of neural networks

Maisonneuve

Vieira²,

Larramendy

et al. 2021

Preprint

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Compartmentalized microfluidic chips have demonstrated tremendous potential to create in vitro minimalistic environments for the reproduction of the neural circuitry of the brain. Although the protocol for seeding neural soma in these devices is well known and has been widely used in myriad studies, the accurate control of the number of neurites passing through the microchannels remains challenging. However, the regulation of axonal density among different groups of neurons is still a requirement to assess the inherent structural connectivity between neuronal populations. In this work, we report the effect of microchannel patterning strategies on the modulation of neuronal connectivity by applying dimensional modifications on microchannel-connected microfluidic chambers. Our results show that those strategies can modulate the direction and the number of neuronal projections of passage, therefore regulating the strength of the structural connections between two populations of neurons. With this approach, we provide innovative microfluidic design rules for the engineering of in vitro physiologically relevant neural networks.

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