Rapid measurement of total antioxidant capacity in plants

Gillespie, Kelly M.; Chae, June M; Ainsworth, Elizabeth A.

doi:10.1038/nprot.2007.100

Cited by 204 publications

(142 citation statements)

References 27 publications

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“…Immediately after each A/C i curve was completed, tissue was removed from the leaf using a cork borer and flash frozen in liquid N. One leaf disc (approximately 1.4 cm 2 ) was incubated in 96% (v/v) ethanol for 3 d at 4°C in order to determine chlorophyll content using the equations of Lichtenthaler and Wellburn (1983). Another leaf disc was used to determine the ORAC content following the procedures described by Gillespie et al (2007). A third leaf disc was used to determine Suc content according to the methods of Jones et al (1977).…”

Section: Gold Standard Methods For Measuring Leaf Physiology and Biocmentioning

confidence: 99%

High-Throughput Phenotyping of Maize Leaf Physiological and Biochemical Traits Using Hyperspectral Reflectance

et al. 2016

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High-throughput, noninvasive field phenotyping has revealed genetic variation in crop morphological, developmental, and agronomic traits, but rapid measurements of the underlying physiological and biochemical traits are needed to fully understand genetic variation in plant-environment interactions. This study tested the application of leaf hyperspectral reflectance (l = 500-2,400 nm) as a high-throughput phenotyping approach for rapid and accurate assessment of leaf photosynthetic and biochemical traits in maize (Zea mays). Leaf traits were measured with standard wet-laboratory and gas-exchange approaches alongside measurements of leaf reflectance. Partial least-squares regression was used to develop a measure of leaf chlorophyll content, nitrogen content, sucrose content, specific leaf area, maximum rate of phosphoenolpyruvate carboxylation, [CO 2 ]-saturated rate of photosynthesis, and leaf oxygen radical absorbance capacity from leaf reflectance spectra. Partial least-squares regression models accurately predicted five out of seven traits and were more accurate than previously used simple spectral indices for leaf chlorophyll, nitrogen content, and specific leaf area. Correlations among leaf traits and statistical inferences about differences among genotypes and treatments were similar for measured and modeled data. The hyperspectral reflectance approach to phenotyping was dramatically faster than traditional measurements, enabling over 1,000 rows to be phenotyped during midday hours over just 2 to 4 d, and offers a nondestructive method to accurately assess physiological and biochemical trait responses to environmental stress.

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Section: Gold Standard Methods For Measuring Leaf Physiology and Biocmentioning

confidence: 99%

High-Throughput Phenotyping of Maize Leaf Physiological and Biochemical Traits Using Hyperspectral Reflectance

et al. 2016

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“…Antioxidant capacity of cold tea extract and hot tea extracts was also assessed conducting the ORAC (oxygen radical absorbance capacity) assay, according to the method proposed by Gillespie et al, (14). The initial samples with a concentration of 10000 ppm were diluted in PBS (phosphate buffered saline) 75 mM for cold tea extracts, the dilutions were made as follows: 1:50 for Jaibel The diluted samples were added to 96-well-microplates; 187 μL of fluorescein (80 nM) diluted in PBS (75 mM) were also added to each well.…”

Section: Orac Assaymentioning

confidence: 99%

Comparative Study of the Antioxidant Capacity in Green Tea by Extraction at Different Temperatures of Four Brands Sold in Colombia

Aristizábal

Ortiz

Restrepo-Aristizabal

et al. 2017

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Background: Tea (Camellia sinensis) is the most highly consumed beverage in the world in addition to water. The most common way of preparation is by immersing the tea bag in hot or cold water. In Colombia, it is a recent trend and the market is growing continuously. Objectives: The objective of this study is to compare the antioxidant characteristics of four brands of green tea sold in Colombia at room and hot-temperature in relation to the preparation conditions. Methods: Four commercial brands of green tea (Oriental

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“…ORAC assay for AA-2G, AA-2G, and AA was carried out as described by Gillespie et al, 21) with a slight modification. Briefly, fluorescein (60 nM), antioxidant (12.5 mM), and AAPH (18.75 mM) were incubated in 200 ml of KH 2 PO 4 -K 2 HPO 4 buffer (75 mM, pH 7.0) at 37 C in a 96-well plate (Costar no.…”

Section: Abtsmentioning

confidence: 99%