Rapid mechanochemical encapsulation of biocatalysts into robust metal–organic frameworks

Wei, Tz Han; Wu, Shi Hong; Huang, Yanli; Lo, Wei Shang; Williams, Benjamin P.; Chen, Sheng Yu; Yang, Hsun Chih; Hsu, Yu Shen; Lin, Zih Yin; Chen, Xin Hua; Kuo, Pei En; Chou, Lien‐Yang; Tsung, Chia Kuang; Shieh, Fa Kuen

doi:10.1038/s41467-019-12966-0

Cited by 162 publications

(102 citation statements)

References 61 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…[73,170] Advantages of mechanochemical reaction are solvent-free condition, [170] reduced reaction time, [171] and potential scale-up to industrial level. [172,173] One of the mechanochemical method, i.e., ball milling was successfully applied in MOF synthesis, [174][175][176] where metal oxides were usually employed as an initial reactant instead of metal ion salts, e.g., zinc oxide was utilized to produce zinc nitrate. [177,178] An early research performed a liquid-assisted grinding via the ball milling method to synthesize homo-and hetero-rare-earth MOFs from trivalent metal carbonates and benzene-1,3,5-tricarboxylic acid in small amount of DMF or water within only 20 min.…”

Section: Mechanochemical Methodsmentioning

confidence: 99%

Metal-organic Framework-based Materials: Synthesis, Stability and Applications in Food Safety and Preservation

Nong¹,

Liu²,

Wang³

et al. 2020

ES Food Agroforest

View full text Add to dashboard Cite

Investigation on food safety and preservation is essential to provide high quality and safe food for human beings, including accurate determination and efficient removal of inorganic heavy metal ions and organic contaminants, as well as regulation of food postharvest ripening and intelligent sterilization. In the last two decades, metal-organic frameworks (MOFs), as functionalized porous materials, have aroused interests of researchers because of their advantages of high porosity, large specific surface area, flexible structure properties, and abundant binding sites for guest molecules. MOFs have shown great potentials in practical applications in food and related fields. In this overview, we summarized the MOF crystallization mechanisms, synthesis routes and methods, stability, as well as the applications in food contamination including removal of heavy metal ions, dyes, and antibiotics, and food preservation including regulation of fruit and vegetable ripening, removal of moisture and oxygen, and high-efficiency sterilization. Finally, two perspectives focusing on the development of MOFs for innovative food research, i.e., food-grade MOFs and MOF composites are suggested based on our understanding.

show abstract

Section: Mechanochemical Methodsmentioning

confidence: 99%

Metal-organic Framework-based Materials: Synthesis, Stability and Applications in Food Safety and Preservation

Nong¹,

Liu²,

Wang³

et al. 2020

ES Food Agroforest

View full text Add to dashboard Cite

show abstract

“…The introduction of enzyme does not inhibit the formation of the MOF crystal and sterically trapped enzyme in the frameworks ( Figure 2 d). It also overcomes the limitation of undersized apertures, 33 especially for those MOFs requiring harsh synthetic conditions (organic solvent/acidic solution) and that only have a trace demand for solvent. More importantly, the rapid synthetic process will minimize enzyme exposure time to those enzyme denaturation factors.…”

Section: Historical Development Of Enzyme Immobilization Strategies Amentioning

confidence: 99%

Metal–Organic Frameworks for Enzyme Immobilization: Beyond Host Matrix Materials

2020

View full text Add to dashboard Cite

Enzyme immobilization in metal–organic frameworks (MOFs) as a promising strategy is attracting the interest of scientists from different disciplines with the expansion of MOFs’ development. Different from other traditional host materials, their unique strengths of high surface areas, large yet adjustable pore sizes, functionalizable pore walls, and diverse architectures make MOFs an ideal platform to investigate hosted enzymes, which is critical to the industrial and commercial process. In addition to the protective function of MOFs, the extensive roles of MOFs in the enzyme immobilization are being well-explored by making full use of their remarkable properties like well-defined structure, high porosity, and tunable functionality. Such development shifts the focus from the exploration of immobilization strategies toward functionalization. Meanwhile, this would undoubtedly contribute to a better understanding of enzymes in regards to the structural transformation after being hosted in a confinement environment, particularly to the orientation and conformation change as well as the interplay between enzyme and matrix MOFs. In this Outlook, we target a comprehensive review of the role diversities of the host matrix MOF based on the current enzyme immobilization research, along with proposing an outlook toward the future development of this field, including the representatives of potential techniques and methodologies being capable of studying the hosted enzymes.

show abstract

“…The cell membrane is a natural barrier separating the extracellular environment from the intracellular components (4). Therefore, improving membrane function is a potential strategy to enhance the growth performance of industrial strains under harsh industrial conditions (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Sphingolipid Synthesis Improves Osmotic Tolerance of Saccharomyces cerevisiae

Zhu

Yin

Luo

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

To enhance the growth performance of Saccharomyces cerevisiae under osmotic stress, mutant XCG001, which tolerates up to 1.5 M NaCl, was isolated through adaptive laboratory evolution (ALE). Comparisons of the transcriptome data of mutant XCG001 and the wild-type strain identified ELO2 as being associated with osmotic tolerance. In the ELO2 overexpression strain (XCG010), the contents of inositol phosphorylceramide (IPC; t18:0/26:0), mannosylinositol phosphorylceramide [MIPC; t18:0/22:0(2OH)], MIPC (d18:0/22:0), MIPC (d20:0/24:0), mannosyldiinositol phosphorylceramide [M(IP)2C; d20:0/26:0], M(IP)2C [t18:0/26:0(2OH)], and M(IP)2C [d20:0/26:0(2OH)] increased by 88.3 times, 167 times, 63.3 times, 23.9 times, 27.9 times, 114 times, and 208 times at 1.0 M NaCl, respectively, compared with the corresponding values of the control strain XCG002. As a result, the membrane integrity, cell growth, and cell survival rate of strain XCG010 increased by 24.4% ± 1.0%, 21.9% ± 1.5%, and 22.1% ± 1.1% at 1.0 M NaCl, respectively, compared with the corresponding values of the control strain XCG002 (wild-type strain with a control plasmid). These findings provided a novel strategy for engineering complex sphingolipids to enhance osmotic tolerance. IMPORTANCE This study demonstrated a novel strategy for the manipulation of membrane complex sphingolipids to enhance S. cerevisiae tolerance to osmotic stress. Elo2, a sphingolipid acyl chain elongase, was related to osmotic tolerance through transcriptome analysis of the wild-type strain and an osmosis-tolerant strain generated from ALE. Overexpression of ELO2 increased the content of complex sphingolipid with longer acyl chain; thus, membrane integrity and osmotic tolerance improved.

show abstract

Rapid mechanochemical encapsulation of biocatalysts into robust metal–organic frameworks

Cited by 162 publications

References 61 publications

Metal-organic Framework-based Materials: Synthesis, Stability and Applications in Food Safety and Preservation

Metal-organic Framework-based Materials: Synthesis, Stability and Applications in Food Safety and Preservation

Metal–Organic Frameworks for Enzyme Immobilization: Beyond Host Matrix Materials

Enhancement of Sphingolipid Synthesis Improves Osmotic Tolerance of Saccharomyces cerevisiae

Contact Info

Product

Resources

About