1950
DOI: 10.1139/cjr50b-013
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Rapid Method for Estimation of Glycerol in Fermentation Solutions

Abstract: The glycerol is quantitatively oxidized to formaldehyde by periodic acid, using a five minute oxidation period to minimize oxidation of glucose. The iodate and periodate are reduced to iodide by a large excess of sodium arsenite and the formaldehyde then determined directly in the oxidation mixture, by the color reaction with chromotropic acid. The precision of the method is about ± 2% in the range 2 to 8 p.p.m. of glycerol. The error due to glucose, in quantity equal to the glycerol, is only 2.5 to 5.0% and c… Show more

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Cited by 463 publications
(112 citation statements)
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“…Glycerol concentrations in the fermentation media were determined by periodate -chromotropic acid analysis method (10,20). Supernatant of centrifuged samples were used for glycerol analysis spectrophotometrically at 570 nm.…”
Section: Glycerol Analysismentioning
confidence: 99%
“…Glycerol concentrations in the fermentation media were determined by periodate -chromotropic acid analysis method (10,20). Supernatant of centrifuged samples were used for glycerol analysis spectrophotometrically at 570 nm.…”
Section: Glycerol Analysismentioning
confidence: 99%
“…Phospholipids were determined in either the Folch extract or the plasma by the method of Zilversmit and Davis (1950). A direct chemical method for estimating triglycerides was employed using the extraction procedure of Oarlson (1963) combined with the hydrolysis procedure of van Handel and Zilversmit (1957), the resultant glycerol content being determined according to the method of Lambert and Neish (1950).…”
Section: (B) Analytical Proceduresmentioning
confidence: 99%
“…Estimation of liver triglyceride was done by a combination of the procedures previously described. 17 ) …”
Section: Oxidation Of L_[35 S]methionine To Its Sulfoxide L-mentioning
confidence: 99%