Rapid method for identifying highly productive varieties of cotton by a selective process

Abstract

Screening of genetically enhanced cotton varieties with a high content of protein markers, proteinase inhibitors, was carried out for the first time. It was found that only 5 of 68 studied cotton selection lines were resistant to wilt, black root rot, and gummosis.

Comparison of Proteinase Inhibitors from the New Gossypol-Free Cotton Variety Bukhara-9

“…Its inhibiting activity was determined by the literature method [3]. The obtained protein solutions were dialyzed in tap water for 24 h in order to determine quantitatively the bound amino-acid content.…”

“…The highly inhibitory lines identified by us were valuable for selection because of a number of morphological changes (early ripening, harvest, fiber yield and length, boll size, mass of 1000 seeds, oil content) [3]. In continuation of research on cotton-seed marker proteins, we isolated and characterized proteinase inhibitors from the new regionalized variety Bukhara-9 and varieties UzFA-705 and Mirishkor, in which the gossypol level was <1% [4].…”

“…The total inhibiting fraction (TIF) of serine (A, C) and thiol (B) proteinase inhibitors was isolated from the Me 2 CO powder of the three new cotton varieties by the published method [3]. The inhibitory activity of the lyophilized proteins was determined using a method modified by us [5].…”