Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification

Butte, W.

doi:10.1016/s0021-9673(01)87931-0

Cited by 236 publications

(138 citation statements)

References 9 publications

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“…The released FAs were transformed into trimethylsilyl ether derivatives using N,O-bis-trimethylsilyl-trifluoroacetamide. In a second method, lipids were transesterified by treatment with trimethylsulfoniumhydroxide in methanol modified from Butte (1983). Quantification of saponified whole-cell core lipid concentrations were measured by gas chromatography-flame ionization detector (Supplementary methods S1.1, Table 1, Supplementary Figure 2).…”

Section: Lipid Analysismentioning

confidence: 99%

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

et al. 2015

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Marine microbial communities experience daily fluctuations in light and temperature that can have important ramifications for carbon and nutrient cycling. Elucidation of such short time scale community-wide dynamics is hindered by system complexity. Hypersaline aquatic environments have lower species richness than marine environments and can be well-defined spatially, hence they provide a model system for diel cycle analysis. We conducted a 3-day time series experiment in a well-defined pool in hypersaline Lake Tyrrell, Australia. Microbial communities were tracked by combining cultivation-independent lipidomic, metagenomic and microscopy methods. The ratio of total bacterial to archaeal core lipids in the planktonic community increased by up to 58% during daylight hours and decreased by up to 32% overnight. However, total organism abundances remained relatively consistent over 3 days. Metagenomic analysis of the planktonic community composition, resolved at the genome level, showed dominance by Haloquadratum species and six uncultured members of the Halobacteriaceae. The post 0.8 μm filtrate contained six different nanohaloarchaeal types, three of which have not been identified previously, and cryo-transmission electron microscopy imaging confirmed the presence of small cells. Notably, these nano-sized archaea showed a strong diel cycle, with a pronounced increase in relative abundance over the night periods. We detected no eukaryotic algae or other photosynthetic primary producers, suggesting that carbon resources may derive from patchily distributed microbial mats at the sediment-water interface or from surrounding land. Results show the operation of a strong community-level diel cycle, probably driven by interconnected temperature, light abundance, dissolved oxygen concentration and nutrient flux effects.

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Section: Lipid Analysismentioning

confidence: 99%

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

et al. 2015

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“…The organic phase was collected and evaporated to dryness. The dried extract was suspended with chloroform and fatty acid methyl esters (FAME) were obtained by transesterification with trimethylsulfoniumhydroxide (Butte, 1983). FAME were separated using a 100 m CP-Sil-88 capillary column (Varian-Chrompack, Darmstadt, Germany), installed in a HP 5890 series II gas chromatograph with a flameionisation detector (Hewlett Packard, Munich, Germany).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Allergic sensitisation and allergic rhinitis are associated with n-3 polyunsaturated fatty acids in the diet and in red blood cell membranes

et al. 2005

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Objective: Due to inconsistent results based on dietary intake data, unsaturated fatty acids in red blood cell (RBC) membranes and diet were used to investigate their association with allergic sensitisation and allergic rhinitis. Design: Cross-sectional, population-based study. Setting: Bavarian Nutrition Survey II (2002-03), Germany. Subjects: A total of 568 adult participants, 325 women and 243 men. Methods: By means of logistic regression models, the relation of fatty acids to (i) allergic sensitisation as defined by means of specific serum immunoglobulin E analysis (CAPSX1 class Z2), and (ii) self-reported allergic rhinitis was examined. Results: A high cell membrane level of eicosapentaenoic acid (EPA, 20:5 n-3) was inversely associated with allergic sensitisation, the adjusted odds ratio (OR) and 95% confidence interval (95% CI) were 0.52 (0.30-0.90) for the highest (vs lowest) quartile. A similar effect was observed for allergic rhinitis with an OR (95% CI) of 0.50 (0.24-1.03; P ¼ 0.027 for trend). A higher dietary intake of alpha-linolenic acid (ALA, 18:3 n-3) was associated with a decreased risk of allergic sensitisation and allergic rhinitis with ORs (95% CIs) of 0.51 (0.28-0.93) and 0.43 (0.20-0.93), respectively, in the highest quartiles. No other dietary or cell membrane unsaturated fatty acid was significantly associated with the outcome variables, nor was the n-6/n-3 ratio. The strongest effects were observed among subjects under the age of 40 y. Conclusions: In this cross-sectional study among adults, a high content of n-3 fatty acids in RBC membranes (EPA) or in the diet (ALA) is associated with a decreased risk of allergic sensitisation and allergic rhinitis. Sponsorship: The study was supported by funds of the Kurt-Eberhard-Bode-Stiftung and the Bavarian Ministry of Environment, Health and Consumer Protection.

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“…The application of chemical ionisation as opposed to electron impact ionisation allowed the analysis of a large number of chemically defined metabolites from the complex biological matrix [14]. Owing to the hypothesis-generating nature of the screening experiment and in order to avoid the introduction of contaminants, no antioxidant or chelate forming additives were used and a single step method of extraction/methylation [9][10][11] was used, that minimized the number of sample handling steps. Larger samples sizes and improved technologies will lead to a more complete understanding of the resulting molecular pattern in the future.…”

Section: Discussionmentioning

confidence: 99%

“…A one step extraction/methylation method using trimethylsulphonium hydroxide (TMSH) [9][10][11] as agent for methylation and protection from further metabolic activity in the sample was used as follows: Ten milligrams of frozen tissue were ground with 5 mm stainless steel balls using liquid nitrogen cooling (MM301 Mixer Mill,RetschH, Haan, Germany) for 30 seconds to a fine powder. For blank samples, all steps were performed identically in parallel to the patient samples, but without tissue.…”

Section: Sample Preparation and Derivatisation For Metabolite Analysismentioning

confidence: 99%

“…The ground powder was suspended by adding 200 ml methanol (Merck, Darmstadt, Germany) at 220uC and transferred to holding tubes where it remained for up to 15 minutes using gentle agitation on a block cooled to 220uC with dry ice. The fully suspended vial content was then transferred into a tapered 1.1 ml GC vial with screw cap (1.1-STVG, 8-ST15, Thermo Fischer Inc., Waltham, MA, USA) and the solution was immediately supplemented with 40 ml 25% TMSH in methanol (Jenachem TM , Jena, Germany) as a methylating agent followed by 15 minutes of incubation at 100uC for derivatisation [9]. To reduce the pressure load on the glass vials and to reduce evaporation, each vial was heated within a 50 ml plastic tube (Falcon TM , BD Biosciences, Heidelberg, Germany) containing 100 ml of methanol as an outer vial.…”

Section: Sample Preparation and Derivatisation For Metabolite Analysismentioning

confidence: 99%

See 1 more Smart Citation

Metabolic signature of electrosurgical liver dissection

Schönfels¹,

Kampen²,

Patsenker³

et al. 2013

Z Gastroenterol

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Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification

Cited by 236 publications

References 9 publications

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

Allergic sensitisation and allergic rhinitis are associated with n-3 polyunsaturated fatty acids in the diet and in red blood cell membranes

Metabolic signature of electrosurgical liver dissection

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