Rapid Method for the Determination of Fat in Feces

Kamer, J. H. Van De; Huinink, H. ten Bokkel; Weyers, H. A.

doi:10.1016/s0021-9258(18)57092-7

Cited by 1,684 publications

(78 citation statements)

References 12 publications

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“…Other studies have reported pancreatic steatorrhea at a rate of approximately 10%, without the presence of diarrhea [14], suggesting the presence of ambiguity in their diagnosis. The Van de Kamer method [15], which is considered the gold standard for the evaluation of exocrine function, can evaluate steatorrhea quantitatively but is troublesome because all stool needs to be collected for 3 days after ingesting 100 grams of fat-restricted diet for 5 days. Consequently, we did not use this diagnostic method in the present study.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Outcomes of Pancreatic Function Following Pancreatic Trauma

et al. 2016

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The objective of this study is to retrospectively assess long-term outcomes and late complications of pancreatic trauma. We studied 14 patients with pancreatic trauma who were treated at the Advanced Emergency Medical Service Center, Kurume University Hospital, between 1981 and 2012 and discharged alive. Relevant data were extracted from patient records and a retrospective patient questionnaire and blood test were completed to evaluate pancreatic function. The median patient age at the time of the survey was 49 years; the median post-injury period was 23 years and 5 months. The comorbidity rates for pancreatic endocrine and exocrine dysfunctions were 35.7% and 33.3%, respectively. No new-onset diabetes mellitus (DM) was seen within 3 years of trauma, except in 1 patient who underwent pancreaticoduodenectomy. DM developed >15 years after trauma in 2 patients each in the pancreatectomy and non-pancreatectomy groups. Diarrhea exacerbated by fat intake was seen in 3 and 1 patient in the pancreatectomy and non-pancreatectomy groups, respectively. Both complications were more common in the pancreatectomy group, but without statistical significance. Although post-surgical pancreatic dysfunction may be absent at discharge, treatment for pancreatic trauma should take into account the possibility that pancreatectomy may accelerate DM onset.

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Section: Discussionmentioning

confidence: 99%

Long-Term Outcomes of Pancreatic Function Following Pancreatic Trauma

et al. 2016

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“…Fecal free fatty acid was analysed by the method of van de Kamer 23 modified by Jeejeebhoy. 24 In brief, fecal lipids were extracted three times with heptane:diethyl ether:ethanol (1:1:1, v/v) (10 mL × 2) and the upper phase consisted of heptane:diethyl ether:ethanol:deionized water (1:1:1:1, v/v) (10 mL × 1) from 1 g of powdered feces acidified with two drops of concentrated HCl.…”

Section: Methodsmentioning

confidence: 99%

Dietary kakrol (Momordica dioica Roxb.) flesh inhibits triacylglycerol absorption and lowers the risk for development of fatty liver in rats

Sato

Ueda

Nagata

et al. 2011

Exp Biol Med (Maywood)

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Kakrol (Momordica dioica Roxb.) is a cucurbitaceous vegetable native to India and Bangladesh. Bitter gourd (Momordica charantia Linn.), a species related to kakrol, has been shown to have pharmacological properties including antidiabetic and antisteatotic effects. In this study, we investigated the effect of dietary kakrol on lipid metabolism in rats. Sprague-Dawley rats were fed AIN-76 formula diets containing 3% freeze-dried powders of whole kakrol or bitter gourd for two weeks. Results showed significantly lowered liver cholesterol and triacylglycerol levels in rats fed on both diets. Fecal lipid excretion increased in rats fed the kakrol diet, and lymphatic transport of triacylglycerol and phospholipids decreased in rats fed the kakrol diet after permanent lymph cannulation. Furthermore, n-butanol extract from kakrol caused a significant concentration-dependent decrease in the pancreatic lipase activity in vitro. These results indicate that the mechanisms of action on lipid metabolism in kakrol and bitter gourd are different and that dietary kakrol reduces liver lipids by inhibiting lipid absorption.

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“…After thawing, the 2-day stool samples were pooled and analyses were performed on homogenized samples. Nitrogen, lipid, and total energy content were determined by nitrogen elemental analysis (18) (Elemental Analyser CHN EA1112; Thermo Scientific), the method of van de Kamer (33), and bomb calorimetry (PARR 1351 Bomb Calorimeter; Parr Instrument), respectively. The energy derived from carbohydrates was calculated by subtracting the energy associated with the nitrogen and lipid components from the total energy.…”

Section: Stool Analysesmentioning

confidence: 99%

Malabsorption and intestinal adaptation after one anastomosis gastric bypass compared with Roux-en-Y gastric bypass in rats

Cavin

Voitellier

Cluzeaud

et al. 2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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The technically easier one-anastomosis (mini) gastric bypass (MGB) is associated with similar metabolic improvements and weight loss as the Roux-en-Y gastric bypass (RYGB). However, MGB is controversial and suspected to result in greater malabsorption than RYGB. In this study, we compared macronutrient absorption and intestinal adaptation after MGB or RYGB in rats. Body weight and food intake were monitored and glucose tolerance tests were performed in rats subjected to MGB, RYGB, or sham surgery. Carbohydrate, protein, and lipid absorption was determined by fecal analyses. Intestinal remodeling was evaluated by histology and immunohistochemistry. Peptide and amino acid transporter mRNA levels were measured in the remodeled intestinal mucosa and those of anorexigenic and orexigenic peptides in the hypothalamus. The MGB and RYGB surgeries both resulted in a reduction of body weight and an improvement of glucose tolerance relative to sham rats. Hypothalamic orexigenic neuropeptide gene expression was higher in MGB rats than in RYGB or sham rats. Fecal losses of calories and proteins were greater after MGB than RYGB or sham surgery. Intestinal hyperplasia occurred after MGB and RYGB with increased jejunum diameter, higher villi, and deeper crypts than in sham rats. Peptidase and peptide or amino acid transporter genes were overexpressed in jejunal mucosa from MGB rats but not RYGB rats. In rats, MGB led to greater protein malabsorption and energy loss than RYGB. This malabsorption was not compensated by intestinal overgrowth and increased expression of peptide transporters in the jejunum.

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Rapid Method for the Determination of Fat in Feces

Cited by 1,684 publications

References 12 publications

Long-Term Outcomes of Pancreatic Function Following Pancreatic Trauma

Long-Term Outcomes of Pancreatic Function Following Pancreatic Trauma

Dietary kakrol (Momordica dioica Roxb.) flesh inhibits triacylglycerol absorption and lowers the risk for development of fatty liver in rats

Malabsorption and intestinal adaptation after one anastomosis gastric bypass compared with Roux-en-Y gastric bypass in rats

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