Rapid methods for deoxynivalenol and other trichothecenes

Schneider, Elisabeth; Curtui, Valeriu; Seidler, C.; Dietrich, Richard; Usleber, Ewald; Märtlbauer, Erwin

doi:10.1016/j.toxlet.2004.04.038

Cited by 79 publications

(50 citation statements)

References 39 publications

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“…This indicated that 3-Ac-DON could be a strong interference for the accurate measurement of DON. Such cross-reactivities against 3-Ac-DON are common drawback of other antibody based detection methods for DON (Maragos and McCormick, 2000;Schneider et al, 2004;Kolosova et al, 2007). The low sensitivity of the assay for 15-Ac-DON relative to 3-Ac-DON indicates that modification of DON at the C-15 position inhibited binding, while modification at the C-3 position did not.…”

Section: Resultsmentioning

confidence: 99%

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Romanazzo

Ricci

Volpe

et al. 2010

Biosensors and Bioelectronics

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a b s t r a c tA reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC 50 of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with the exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analyzing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products.

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Section: Resultsmentioning

confidence: 99%

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Romanazzo

Ricci

Volpe

et al. 2010

Biosensors and Bioelectronics

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“…Existing bioassays for the detection of type A trichothecenes take advantage of cross-reactive antibodies that recognise both T-2 and HT-2 (Molinelli et al, 2008;Park and Chu, 1996;Schneider et al, 2004). With the present report, we propose an alternative screening strategy based on the ability of type A trichothecenes to induce distinctive changes of transcription in easy-to-culture human epithelial cells.…”

Section: Discussionmentioning

confidence: 96%

“…Thus, to reduce human exposure to these hazardous contaminants, it is necessary to screen a wide range of raw and manufactured foods that are consumed on a daily basis. To date, reliable screening systems for type A trichothecenes are dependent on the availability of immunoassays using specific antibodies against T-2 and HT-2 (Molinelli et al, 2008;Park and Chu, 1996;Schneider et al, 2004). Here, we propose an alternative screening strategy based on the ability of these compounds to re-programme the genome of routinely cultured human epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic-based bioassays for the detection of type A trichothecenes

Lancová

Bowens

Stroka

et al. 2009

WMJ

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The type A trichothecenes T-2 toxin (T-2) and HT-2 toxin (HT-2) are hazardous Fusarium products that contaminate many field crops growing in cold to temperate regions across the world. Toxicity studies in laboratory and farm animals have been used to derive a temporary tolerable daily intake (t-TDI) for the sum of T-2 and HT-2 of no more than 60 ng/kg body weight. To protect the consumers, it is now necessary to screen a large number of food samples for the presence of these poisonous fungal metabolites. Towards that goal, we discovered that the transcriptional apparatus of a human carcinoma cell line (MCF7) provides a sensitive biological sensor of type A trichothecenes. In fact, exposure of this easy-to-culture cell line to T-2 or HT-2 results in the regulation of >2,000 different transcripts with expression changes ranging from >5,000-fold gene inductions to >40-fold gene repressions. These transcriptional responses have been exploited to develop practical microchip and reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of type A trichothecenes at parts per billion levels.

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“…cell adhesion [64], innate immunity [3], CO 2 assimilation in metabolic pathways [30], extracellular matrix degradation [2] and membrane construction [17,44,85]) as well as the binding properties of retrocyclins [14], apotosis regulators [42], antimicrobial peptides [23], glycosphingolipids [97], barley -amylase/subtilisin inhibitor [66], amelogenin [67], lysenin [45], lectins [65], endoxylases [40], connexins [21], selectins [25], phosphoinositides [71], carbohydrates [20,70,72], HIV proteins [48,91], tumor necrosis factor receptor superfamily [95] and the insulin-like growth factor system [58]. Other reviews highlight the integration of optical biosensors with complementary technologies in areas of applied research, including drug development and chemical genetics [1,50,73,83,88], plant proteomics [33], gene and protein expression [29,35,86], antibody optimization [5,46,62], food industry [4,47,54,61,79], bioprocessing and manufacturing [12,…”

Section: Reviews Theory and Methodsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

115

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The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

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Rapid methods for deoxynivalenol and other trichothecenes

Cited by 79 publications

References 39 publications

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Transcriptomic-based bioassays for the detection of type A trichothecenes

Survey of the year 2004 commercial optical biosensor literature

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