Rapid methods in the assessment of microbiological quality; experiences and needs

Jarvis, Basil; Easter, M.C.

doi:10.1111/j.1365-2672.1987.tb03617.x

Cited by 16 publications

(6 citation statements)

References 13 publications

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“…This is particularly important for some of the newer rapid methods which are sometimes based on non-microbiological concepts that may be difficult to comprehend. Accordingly, the need for training in the methodology and the realization of its limitations are paramount (Jarvis & Easter, 1987).…”

Section: Discussionmentioning

confidence: 99%

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Easter

Kroll²,

Farr³

et al. 1987

Int J of Dairy Tech

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In the light of experiences gained and observations made in the dairy industry, the critical control points and factors that affect the operation of the DEFT (Direct Epifluorescent Filter Technique) in a routine laboratory were determined. Recommendations are made for the optimization of both the test protocol and the instrumentation, and the need for adequate operator training is strongly emphasized.

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Section: Discussionmentioning

confidence: 99%

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Easter

Kroll²,

Farr³

et al. 1987

Int J of Dairy Tech

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“…However, a method that may be deemed rapid in application with a high bioburden may require an extended enrichment period in situations of lower or injured bioburden. Useful discussion and comparison of the principal methods can be found in the following works: general aspects Jarvis and Easter, 1987;Balows et al, 1989;Stannard et al, 1989;Blackburn, 1993;Watling and Leech, 1996;Stewart, 1997;Geis, 2006 , adenosine triphosphate ATP bioluminescence Jago et al, 1989;Stanley et al, 1989;Stewart et al, 1989;Stewart, 1990;Stewart, 1997;Dexter et al, 2003 , fluorescent …”

Section: Currently Available Rapid Methods and Their Brief Explanationmentioning

confidence: 99%

Methods of Rapid Microbiological Assay and Their Application to Pharmaceutical and Medical Device Fabrication

SHINTANI

2016

Biocontrol Sci.

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There are several rapid microbiological methods becoming available that have useful applications in pharmaceutical and medical devices. They are ATP bioluminescence, fluorescent labeling, electrical resistance, and nucleic acid probes. In choosing to employ rapid methods, the microbiologist should examine their prospective performances against the specific requirements for that sector. Some methods may require expensive equipment and offer full automation, and others represent only a small investment. The regulatory view of these methods is changing and they still officially have not been approved in medical and pharmaceutical area, but it will still be up to the microbiologist to demonstrate that the method chosen is fit for the purpose intended.

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“…There has been much research devoted to Salmonella methods in order to improve its sensitivity, and selectivity and to reduce the time taken to obtain a result. Considerable advances have been made using new technology such as immunoassays, electrical measurement and DNA probes (DAoust 1981;Jarvis & Easter 1987). A combined immuno-electrical technique was demonstrated by Bird et al (1989) but was not optimized.…”

mentioning

confidence: 99%

The detection of Salmonella enteritidis and S. typhimurium using immunomagnetic separation and conductance microbiology

Parmar

Easter²,

Forsythe

1992

Lett Appl Microbiol

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A rapid method for detection of Salmonella in milk powder is described. The technique involves immunomagnetic separation of Salmonella from pre-enrichment broths using new commercially-available materials, and detection using conductance measurements. Salmonella detection was enhanced by reducing the number and types of competing bacteria present and concentrating the number of Salmonella in the final assay. After a 6 h pre-incubation period Salmonella enteritidis, from an initial inoculum size of 20 cells/ml, were detected in 7.5 h by conductance.

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Rapid methods in the assessment of microbiological quality; experiences and needs

Cited by 16 publications

References 13 publications

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Methods of Rapid Microbiological Assay and Their Application to Pharmaceutical and Medical Device Fabrication

The detection of Salmonella enteritidis and S. typhimurium using immunomagnetic separation and conductance microbiology

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