Rapid microfabrication of solvent-resistant biocompatible microfluidic devices

Hung, Lung-Hsin; Lin, Robert; Lee, Abraham P.

doi:10.1039/b717710k

Cited by 108 publications

(97 citation statements)

References 27 publications

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“…The junctions between the two main channels and CC will be abbreviated as MC1-CC and CC-MC2 respectively. The micro-channel was fabricated using NOA 81 (Norland Products Inc.) ultraviolet-curable adhesive based on the method developed by Hung et al 16 Briefly, a poly(dimethylsiloxane) (PDMS) mold of the micro-channel was casted from a SU-8 (MicroChem Corp.) negative mold fabricated by photolithography. 17 NOA 81 micro-channel was casted from the PDMS mold and semi-cured by ultraviolet light.…”

Section: -mentioning

confidence: 99%

Chaos analysis of viscoelastic chaotic flows of polymeric fluids in a micro-channel

2015

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Many fluids, including biological fluids such as mucus and blood, are viscoelastic. Through the introduction of chaotic flows in a micro-channel and the construction of maps of characteristic chaos parameters, differences in viscoelastic properties of these fluids can be measured. This is demonstrated by creating viscoelastic chaotic flows induced in an H-shaped micro-channel through the steady infusion of a polymeric fluid of polyethylene oxide (PEO) and another immiscible fluid (silicone oil). A protocol for chaos analysis was established and demonstrated for the analysis of the chaotic flows generated by two polymeric fluids of different molecular weight but with similar relaxation times. The flows were shown to be chaotic through the computation of their correlation dimension (D2) and the largest Lyapunov exponent (λ1), with D2 being fractional and λ1 being positive. Contour maps of D2 and λ1 of the respective fluids in the operating space, which is defined by the combination of polymeric fluids and silicone oil flow rates, were constructed to represent the characteristic of the chaotic flows generated. It was observed that, albeit being similar, the fluids have generally distinct characteristic maps with some similar trends. The differences in the D2 and λ1 maps are indicative of the difference in the molecular weight of the polymers in the fluids because the driving force of the viscoelastic chaotic flows is of molecular origin. This approach in constructing the characteristic maps of chaos parameters can be employed as a diagnostic tool for biological fluids and, more generally, chaotic signals.

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Section: -mentioning

confidence: 99%

Chaos analysis of viscoelastic chaotic flows of polymeric fluids in a micro-channel

2015

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“…[19,20] Thus, the fabrication of solvent-resistant microfluidic devices is a prerequisite for the formation of organic droplets if we are to realize droplet-based chemical reactions. [7,9] We present here a simple and easy method for the stable surface modification of PDMS using a solvent-resistant organic/inorganic hybrid material (HR4) to provide solvent compatibility, while the inherent bulk properties of PDMS are retained for the rapid prototyping of microfluidic devices. [21] First, the PDMS surface was treated with oxygen plasma to produce a silicate layer and then it was reacted with HR4 using covalent siloxane coupling.…”

mentioning

confidence: 99%

Solvent‐Resistant PDMS Microfluidic Devices with Hybrid Inorganic/Organic Polymer Coatings

Kim

Hong

Chung

et al. 2009

Adv Funct Materials

102

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This study presents a method for the fabrication of solvent‐resistant poly(dimethylsiloxane) (PDMS) microfluidic devices by coating the microfluidic channel with a hybrid inorganic/organic polymer (HR4). This modification dramatically increases the resistance of PDMS microfluidic channels to various solvents, because it leads to a significant reduction in the rate of solvent absorption and consequent swelling. The compatibility of modified PDMS with a wide range of solvents is investigated by evaluating the swelling ratio measured through weight changes in a standard block. The HR4‐modified PDMS microfluidic device can be applied to the formation of water‐in‐oil (W/O) and oil‐in‐water (O/W) emulsions. The generation of organic solvent droplets with high monodispersity in the microfluidic device without swelling problems is demonstrated. The advantage of this proposed method is that it can be used to rapidly fabricate microfluidic devices using the bulk properties of PDMS, while also increasing their resistance to various organic solvents. This high compatibility with a variety of solvents of HR4‐modified PDMS can expand the application of microfluidic systems to many research fields.

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“…14 Their method used direct photolithography to pattern the thiol-ene; however, soft-lithography of thiol-enes has since been demonstrated in numerous applications including droplet generation, 15 soft-lithography stamps, 16 microchip capillary electrophoresis, 17 cell culture, 18 and optofluidics. [19][20][21] OSTE polymers can be directly bonded using the reactive groups present on the surface.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of rigid microstructures with thiol-ene-based soft lithography for continuous-flow cell lysis

et al. 2014

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In this work, we introduce a method for the soft-lithography-based fabrication of rigid microstructures and a new, simple bonding technique for use as a continuousflow cell lysis device. While on-chip cell lysis techniques have been reported previously, these techniques generally require a long on-chip residence time, and thus cannot be performed in a rapid, continuous-flow manner. Microstructured microfluidic devices can perform mechanical lysis of cells, enabling continuous-flow lysis; however, rigid silicon-based devices require complex and expensive fabrication of each device, while polydimethylsiloxane (PMDS), the most common material used for soft lithography fabrication, is not rigid and expands under the pressures required, resulting in poor lysis performance. Here, we demonstrate the fabrication of microfluidic microstructures from off-stoichiometry thiol-ene (OSTE) polymer using soft-lithography replica molding combined with a post-assembly cure for easy bonding. With finite element simulations, we show that the rigid microstructures generate an energy dissipation rate of nearly 10 7 , which is sufficient for continuous-flow cell lysis. Correspondingly, with the OSTE device we achieve lysis of highly deformable MDA-MB-231 breast cancer cells at a rate of 85%, while a comparable PDMS device leads to a lysis rate of only 40%. V C 2014 AIP Publishing LLC.

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Rapid microfabrication of solvent-resistant biocompatible microfluidic devices

Cited by 108 publications

References 27 publications

Chaos analysis of viscoelastic chaotic flows of polymeric fluids in a micro-channel

Chaos analysis of viscoelastic chaotic flows of polymeric fluids in a micro-channel

Solvent‐Resistant PDMS Microfluidic Devices with Hybrid Inorganic/Organic Polymer Coatings

Fabrication of rigid microstructures with thiol-ene-based soft lithography for continuous-flow cell lysis

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