Rapid miniprep of DNA from filamentous fungi.

Leach, J. E.; Finkelstein, David; Rambosek, J A

doi:10.4148/1941-4765.1585

Cited by 63 publications

(40 citation statements)

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“…Total genomic DNA was extracted from the leaves of collected Capparis L. DNA extraction was conducted following a modified protocol of Leach et al (1986). Total DNA concentration was detected by the Spectrophotometer (Gene quant, Amersham Biosciences, USA) and the concentration was adjusted to 20 ng/µL.…”

Section: Molecular Analyses Dna Extractionmentioning

confidence: 99%

Genetic Diversity of Some Capparis L. Species Growing in Syria

Safadi

Faouri

Elias

2014

Braz. arch. biol. technol.

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Section: Molecular Analyses Dna Extractionmentioning

confidence: 99%

Genetic Diversity of Some Capparis L. Species Growing in Syria

Safadi

Faouri

Elias

2014

Braz. arch. biol. technol.

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“…Forty-four strains representative of the uniseriate black aspergilli isolated from grapes were analysed by sequence analysis together with 27 representative strains of section Nigri. Total nucleic acids were isolated as described previously (Leach et al, 1986). Fragments containing the region encoding the internal transcribed spacer 1 (ITS-1), 5.8S rDNA and internal transcribed spacer 2 (ITS-2) were amplified using primers ITS1 and ITS4 as described previously (White et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Aspergillus uvarum sp. nov., an uniseriate black Aspergillus species isolated from grapes in Europe

Perrone

Varga

Susca

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel species, Aspergillus uvarum sp. nov., is described within Aspergillus section Nigri. This species can be distinguished from other black aspergilli based on internal transcribed spacers (ITS), b-tubulin and calmodulin gene sequences, by AFLP analysis and by extrolite profiles. Aspergillus uvarum sp. nov. isolates produced secalonic acid, common to other Aspergillus japonicus-related taxa, and geodin, erdin and dihydrogeodin, which are not produced by any other black aspergilli. None of the isolates were found to produce ochratoxin A. The novel species is most closely related to two atypical strains of Aspergillus aculeatus, CBS 114.80 and CBS 620.78, and was isolated from grape berries in Portugal, Italy, France, Israel, Greece and Spain.

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“…Total nucleic acids were isolated according to the literature (Leach et al, 1986). Fragments containing the ITS region were amplified using primers ITS1 and ITS4 (White et al, 1990).…”

Section: Methodsmentioning

confidence: 99%