Rapid mixed mode solid phase extraction method for the determination of acrylamide in roasted coffee by HPLC–MS/MS

Acrylamide in Coffee and Coffee Substitutes

“…Coffee is actually is one of the most complex matrices, due to the presence of interfering compounds [51,58]. More recently, a rapid and reliable purification method has been developed [61], based on an in-house made single mixed SPE column. Also, a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method based on water extraction and partition with acetonitrile by salting out with MgSO 4 and NaCl has been developed [59].…”

Analysis of Acrylamide in Foods with Special Emphasis on Sample Preparation and Gas Chromatography–Mass Spectrometry Detection

“…Most cleanup procedures consist of the combination of several SPEs to reduce ion suppression or the so-called matrix effects, and to achieve low limit of detection. Bortolomeazzi et al [69] used a single mixed SPE cartridge packed with C18, SCX, and SAX sorbents for the determination of acrylamide in roasted coffee. Ahn et al [3] concluded that the passage of the food sample extract through a mixed-mode SPE cartridge containing apolar, strong cation exchanger and strong anion exchanger functional groups removed any entrained fats and largely eliminated the matrix effects.…”

Analysis of Acrylamide in Coffee

“…The cleanup step could be achieved by using single or multiple SPE with different sorbents (silica, polymer, or carbon-based), or combinations of these (mixed mode). 22 ) functional groups), Bond Elut C18 (C 18 hydrophobic silica-based encapped sorbent), Bond Elut Jr-PSA (anion exchange), Bond Elut Accucat, Bond Elut SCX (silica-based benzene sulphonic acid, nonendcapped), Strata-X-C (Polymeric resin functionalised with polar and strong cation exchange groups), Strata C18E (C 18 hydrophobic silica-based sorbent), Chromabond HR-XC (Polymeric resin functionalised with polar and strong cation exchange groups), Carbograph 4 (graphitised carbon), or Bakerbond SiOH (silica gel based sorbent), among others. Hydroxylated polystyrenedivinylbenzene copolymer phase (ENV+) gives the strongest retention.…”