Rapid monitoring of proline accumulation in paprika leaf sap relative to leaf position and water stress

Bhandari, Shiva Ram; Lee, Min Ji; Rhee, Han Cheol; Choi, Gyeong Lee; Oh, Sang Seok; Lee, Je Taek; Lee, Jun Gu

doi:10.1007/s13580-018-0063-6

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…Freeze-dried powdered leaf tissue (0.1 g) was extracted in distilled water by shaking for 30 minutes. Next, the extract was centrifuged, filtered through a 0.2-µm syringe filter, injected (10 µL) into a 1260 Infinity high-performance liquid Detection of Temperature Stress Using Chlorophyll Fluorescence Parameters and Stress-related Chlorophyll and Proline Content in … chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA, USA), and analyzed according to Bhandari et al (2018). Briefly, pre-column automatic derivatization of proline was performed using borate buffer, 9-fluorenylmethyl chloroformate (FMOC), and o-phthalaldehyde (OPA) at first.…”

Section: Measurement Of Proline Concentrationmentioning

confidence: 99%

Detection of Temperature Stress Using Chlorophyll Fluorescence Parameters and Stress-related Chlorophyll and Proline Content in Paprika (Capsicum annuum L.) Seedlings

Bhandari¹,

Kim²,

Lee³

2018

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Section: Measurement Of Proline Concentrationmentioning

confidence: 99%

Detection of Temperature Stress Using Chlorophyll Fluorescence Parameters and Stress-related Chlorophyll and Proline Content in Paprika (Capsicum annuum L.) Seedlings

Bhandari¹,

Kim²,

Lee³

2018

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“…The contents of soluble sugar, sucrose, AsA, H 2 O 2 and hydroxyl radical scavenging rate were measured utilizing the plant soluble sugar assay kit, Micro Ascorbic Acid content assay Kit, Hydrogen peroxide assay kit, Hydroxyl free radical removal capacity determination kit from Suzhou Comin Biotechnology Co., Ltd. (Suzhou, China), according to the manufacturer's instructions. The content of Pro was measured using an HPLC system [53]. The activity of glycogen synthase (GlgA) was measured according to the instructions for the glycogen synthase kit from Suzhou Comin Biotechnology Co. Ltd. (Suzhou, China).…”

Section: Detection Of Physiological Parametersmentioning

confidence: 99%

Proteome profiling reveals changes in energy metabolism, transport and antioxidation during drought stress in Nostoc flagelliforme

Ding

Wang

et al. 2022

BMC Plant Biol

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Background Drought is an important abiotic stress that constrains the growth of many species. Despite extensive study in model organisms, the underlying mechanisms of drought tolerance in Nostoc flagelliforme remain elusive. Results We characterized the drought adaptation of N. flagelliforme by a combination of proteomics and qRT-PCR. A total of 351 differentially expressed proteins involved in drought stress adaptation were identified. It was found that the expression of several nutrient influx transporters was increased, including molybdate ABC transporter substrate binding protein (modA), sulfate ABC transporter substrate-binding protein (sbp) and nitrate ABC transporter (ntrB), while that of efflux transporters for toxic substances was also increased, including arsenic transporting ATPase (ArsA), potassium transporter (TrkA) and iron ABC transporter substrate-binding protein (VacB). Additionally, photosynthetic components were reduced while sugars built up during drought stress. Non-enzymatic antioxidants, orange carotenoid protein (OCP) homologs, cytochrome P450 (CYP450), proline (Pro) and ascorbic acid (AsA) were all altered during drought stress and may play important roles in scavenging reactive oxygen species (ROS). Conclusion In this study, N. flagelliforme may regulates its adaptation to drought stress through the changes of protein expression in photosynthesis, energy metabolism, transport, protein synthesis and degradation and antioxidation. Highlights • A total of 351 DEPs involved in adaptation to drought stress were identified. • Changes in the expression of six OCP homologs were found in response to drought stress. • Differential expression of transporters played an important role in drought stress adaptation. • Most PSII proteins were downregulated, while PSI proteins were unchanged in response to drought stress. • Sugar metabolism was upregulated in response to drought stress.

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“…작물의 수분 스트레스 정도를 파악하여 정밀 관수를 하기 위한 노력이 계속되어 왔으며, 특히, 기공 개폐에 의하여 영향 을 받는 작물의 엽온을 측정하여 관수 계획을 수립하는 연구가 활발히 진행되었다. 그리고 배지의 수분 함량을 근거로 작물 생 장 최적화 관수 계획을 위한 연구 (Choi and Shin, 2019)와 목부액의 물리•화학적 특성을 분석하여 관수 계획을 수립하는 연구 (Bhandari et al, 2018;Park et al, 2019)도 진행되었다.…”

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Development of Crop Water Stress Index for Kimchi Cabbage Precision Irrigation Control

Lee¹,

Kim²,

Lee³

et al. 2019

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This study was carried out to investigate the effects of soil moisture tension on the growth and leaf temperature of the Kimchi cabbage and develop the crop water stress index (CWSI) for precision irrigation control. Kimchi cabbages were cultivated under different levels (-20, -100, and -500 kPa) of soil moisture tension (SMT). At 60 days after transplanting, the dry matter weight was significantly lower at -500 kPa of SMT than -20 and -100 kPa, however, there were non-significant changes in the maximum leaf length, leaf width, and leaf number among SMT levels. The head weight of the Kimchi cabbage was 60% greater (2,331 g/plant) at -500 kPa than at -20 kPa (1,442 g/plant). The mean leaf temperature of cultivars at -20, -100, and -500 kPa during trial periods was 25.9, 26.5, and 30.6°C, respectively. The CWSI of cultivars at -20, -100, and -500 kPa was 0.0, 0.1, and 1.0, respectively. Results indicated that the growth, leaf temperature, and CWSI calculated from the leaf temperature of the Kimchi cabbage can differ depending on the SMT, and it is possible to develop feasible methods for precision irrigation using the CWSI.

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Rapid monitoring of proline accumulation in paprika leaf sap relative to leaf position and water stress

Cited by 4 publications

References 38 publications

Detection of Temperature Stress Using Chlorophyll Fluorescence Parameters and Stress-related Chlorophyll and Proline Content in Paprika (Capsicum annuum L.) Seedlings

Detection of Temperature Stress Using Chlorophyll Fluorescence Parameters and Stress-related Chlorophyll and Proline Content in Paprika (Capsicum annuum L.) Seedlings

Proteome profiling reveals changes in energy metabolism, transport and antioxidation during drought stress in Nostoc flagelliforme

Development of Crop Water Stress Index for Kimchi Cabbage Precision Irrigation Control

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